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The Possible Participation of the Mesophyll on Stomatal Opening

Lee, Joon-Sang   (Department of Life Science, Sangji UniversityUU0000668  );
  • 초록

    Many researchers have been studied with guard cell protoplasts and detached epidermis as they think that properly stabilized protoplasts and detached epidermis retain many of the properties of intact guard cells. However, some studies have shown that stomata in detached epidermis behave differently, both quantitatively and qualitatively, from those in the intact leaf. Stomata in the intact leaf are very sensitive to environmental factors such as light, $CO_2$ and osmotic stress, but stomata in detached epidermis are less sensitive to these factors than those in the intact leaf. The clearest evidence to suggest the different response between detached epidermis and intact leaf obtained from the experiments with heavy metal, cadmium. 3-weeks old Commelina. communis was transferred to and grown in Hoagland solution in the presence or absence of 5 mM $Cd^{2+}$ for 4 days. The application of $Cd^{2+}$ showed about 70% inhibition of stomatal conductance when measured at various light intensity (100-1,000 $\mu$ mole $m^{-2}s^{-1}). However, stomata in detached epidermis floated on an incubation medium containing 100 $\mu$ M $Cd^{2+}$ opened to a degree of about 8.38 fm, but the stomata treated with no cadmium opened to 3.74 ${\mu}{\textrm}{m}$ . These results were unexpected as the intact leaf grown in a Hoagland solution containing cadmium showed very negative physiological responses. These results showed that stomata in detached epidermis and in the intact leaf could respond reversely. Therefore, it is possible that we now misunderstand how stomata open in real natural condition.


  • 주제어

    Detached epidermis .   Isolated epidermis .   Stomata.  

  • 참고문헌 (33)

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 저자의 다른 논문

  • Lee, Joon-Sang (21)

    1. 1997 "오존에 대한 식물 기공 반응고찰" 한국생태학회지 = The Korean journal of ecology 20 (2): 83~94    
    2. 1997 "닭의장풀의 분리표피에서 $H_2O_2$에 의한 기공 닫힘기작" 한국환경과학회지 = Journal of the environmental sciences 6 (2): 125~131    
    3. 1998 "The Mechanism of Stomatal Closing by Salicylic Acid in Commelina communis L." Journal of plant biology = 식물학회지 41 (2): 97~102    
    4. 1999 "Salicylic acid가 닭의장풀의 광합성에 미치는 영향" 환경생물 = Korean journal of environmental biology 17 (3): 359~364    
    5. 2000 "The Effects of Two Abscisic Acid Analogues, WL19224 and WL19377, on Stomatal Closure" Journal of plant biology = 식물학회지 43 (1): 56~59    
    6. 2000 "닭의장풀 내 $Cd^2+$의 분포와 생리적 독성" 환경생물 = Korean journal of environmental biology 18 (1): 63~67    
    7. 2000 "$Cd^{2+}$이 닭의장풀의 기공개폐에 미치는 영향" 환경생물 = Korean journal of environmental biology 18 (2): 263~268    
    8. 2000 "고등학교 공통 과학 강의에서 교사에 따른 역동적 구조의 정량적 비교 분석" 한국과학교육학회지 = Journal of the Korean association for science education 20 (4): 505~509    
    9. 2001 "닭의장풀에 $Cd^{2+}$처리 시 $Cd^{2+}$ 흡수와 nonprotein-SH 합성에 미치는 살리실릭산의 영향" 환경생물 = Korean journal of environmental biology 19 (3): 218~222    
    10. 2002 "닭의장풀에 $Cd^{2+}$ 처리시 $Cd^{2+}$ 흡수와 생장에 미치는 $Ca^{2+}$의 영향" 환경생물 = Korean journal of environmental biology 20 (1): 40~45    

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