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생물학적 환경정화를 위한 고농도 페놀에서 생육할 수 있는 세균의 분리 및 특성
Isolation and Characterization of Bacteria Able to Grow with Phenol at High Concentrations for Bioremediation

박연규   (밀양대학교 환경공학과UU0000567  ); 손홍주   (밀양대학교 생물공학과UU0000567  );
  • 초록

    For the biological treatment of industrial wastewater containing high concentration of phenol, isolation and characterization of phenol - degrading bacterium were carried out. A bacterial strain P2 capable of degrading phenol was isolated from contaminated soils by enrichment culture technique and identified as the genus Rhodococcus by morphological, cultural, biochemical characteristics, and Biolog system. The optimal medium composition and cultural conditions for the growth and degradation of phenol by Rhodococcus sp. P2 were 0.1% of (NH $_4$ ) $_2$ SO $_4$ , 0.2% of KH $_2$ PO $_4$ , 0.25% of Na $_2$ HPO $_4$ ㆍ12 $H_2O$ , 0.2% of MgSO $_4$ ㆍ7 $H_2O$ , and 0.008% of CaC1 $_2$ ㆍ2 $H_2O$ along with initial pH 8.5 at 3 $0^{\circ}C$ . Rhodococcus sp. P2 could grow with phenol as the sole carbon source up to 1,800 ppm in batch cultures, but did not grow in medium containing above 2,000 ppm of phenol. When 800 ppm phenol was given in the optimal media, Rhodococcus sp. P2 completely degraded it within 24 h. Meanwhile, 1,800 ppm of phenol was degraded within 9 days. Rhodococcus sp. P2 could utilize toluene, n-hexane, xylene and benzene as sole carbon source .


  • 주제어

    phenol .   biodegradation .   wastewater .   biological treatment.  

  • 참고문헌 (18)

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 저자의 다른 논문

  • 박연규 (5)

    1. 1999 "저서성 대형 무척추동물에 의한 밀양강의 생물학적 수질평가" 한국환경과학회지 = Journal of the environmental sciences 8 (1): 7~18    
    2. 2000 "밀양댐 수계의 수서곤충 군집에 대하여" 한국습지학회지 = Journal of wetlands research 2 (2): 133~146    
    3. 2004 "인공기질 부착조류에 의한 대천의 수질평가" 환경생물 = Korean journal of environmental biology 22 (1): 242~245    
    4. 2004 "케라틴 단백질 폐기물의 생물공학적 적용을 위한 우모 분해세균의 분리 및 동정" 생명과학회지 = Journal of life science 14 (2): 229~234    
    5. 2005 "폐광산 주변 토양의 Zn, Cu, Pb의 함량 및 식물체내 축적" 생명과학회지 = Journal of life science 15 (5): 826~833    

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