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Salicylate가 담배 미분화세포 isoperoxidase $A_3$의 활성에 미치는 영향
Effects of Salicylate on the Activity of Isoperoxidase $A_3$ from Tobacco Callus

이미영   (순천향대학교 생명과학부UU0000848  );
  • 초록

    식물의 저항성과 방어관련물질 유도에 관련된 salicylate가 담배 미분화세포로부터 분리된 isoperoxidase $A_3$ 의 활성에 미치는 영향을 살펴보고, $Fe^{2+}$ 의 첨가에 의하여 salicylate에 의한 효소활성 억제가 보호되는 양상을 조사하였다. 다양한 농도의 salicylate가 isoperoxidase $A_3$ 의 활성에 미치는 영향을 조사한 결과 0.48mM salicylate에 의하여 isoperoxidase $A_3$ 의 활성이 약 20% 감소되었고, 0.6mM salicylate에 의하여 효소활성이 85% 이상 감소되었다. 이러한 결과는 salicylate에 의하여 isoperoxiase $A_3$ 가 억제됨을 보여준다. Salicylate에 의한 isoperoxidase $A_3$ 의 억제반응은 pH 의존적으로 일어났을 뿐만 아니라, noncompetitive inhibition 양상을 나타냈다. 뿐만 아니라 salicylate에 의한 isoperoxidase $A_3$ 의 활성억제는 $Fe^{2+}$ 첨가에 의하여 거의 완벽하게 보호되었다. Isoeroxidase A3로부터 heme부분을 제거하여 apoperoxidase를 제조한 후 여러가지 금속이온이 apoperoxidase의 활성회복에 미치는 효과를 조사하였다. 그 결과 hemin과 $Fe^{2+}$ 첨가에 의하여 apoperoxidase의 활성이 80% 이상 회복되었으나 $Cu^{2+},\;Zn^{2+},\;Co^{2+},\;Mn^{2+}$ 등은 apoperoxidase의 활성을 재구성시키지 못하였다.


    Salicylate is involved in the induction of pathogen-related proteins and plant defense response. The effects of salicylate on the activity isoperoxidase $A_3$ from tobacco callus (Nicotiana tabacum L.) and the protection against the enzyme inactivation by salicylate in the presence of $Fe^{2+}$ were examined. About 20% and 85% activity losses of peroxidase occurred at 0.48 mM and 0.6 mM salicylate, respectively, showing that isoperoxidase $A_3$ was inactivated by salicylate. The inactivation occurred depending on pH and showed noncompetitive inhibition mode. Moreover, inactivation of the enzyme by salicylate was completely protected in the presence of $Fe^{2+}$ . Apoperoxidase without heme moiety was constructed and the effects of various metal ions on the recovery of enzyme activities were investigated. More than 80% of the activity was reconstituted by the addition of $Fe^{2+}$ or hemin. However, the enzyme activity was not recovered by $Cu^{2+},\;Zn^{2+},\;Co^{2+},\;or\;Mn^{2+}$ .


  • 주제어

    Salicylate .   tobacco callus .   isoperoxidase $A_3$.  

  • 참고문헌 (25)

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    1. 1997 "염분스트레스에 의한 소나무잎 Peroxidase의 활성 및 Isozyme Pattern의 변화" 한국생태학회지 = The Korean journal of ecology 20 (5): 315~321    
    2. 1997 "Effects of Various Ions on the Cellular and Secretory Isoperoxidases in Rice Suspension Culture" Journal of biochemistry and molecular biology = 한국생화학회지 30 (6): 379~384    
    3. 1998 "지렁이 Peroxidase의 특성 및 페녹시계 제초제의 분해" 한국생태학회지 = The Korean journal of ecology 21 (1): 73~80    
    4. 1998 "Characteristics of a Low Molecular Weight Minor Anionic Isoperoxidase $A_{3n}$ from Radish" Journal of biochemistry and molecular biology = 한국생화학회지 31 (6): 548~553    
    5. 2000 "한국산 무 (Raphanus sativus L.) anionic peroxidase를 이용한 당 정량법 연구" 한국농화학회지 = Journal of the Korean society of Agricultural Chemistry and Biotechnology 43 (2): 100~105    
    6. 2000 "제라늄(Pelargonium graveolense) 캘러스의 재분화 및 peroxidase isozyme 발현패턴 변화" 한국농화학회지 = Journal of the Korean society of Agricultural Chemistry and Biotechnology 43 (3): 184~189    
    7. 2000 "여러 가지 환경스트레스가 벼 유묘 퍼옥시다제 활성에 미치는 영향" 환경생물 = Korean journal of environmental biology 18 (3): 331~336    
    8. 2000 "Peroxidase Activity Boosting by Various Nitrogenous Compounds" Journal of biochemistry and molecular biology = 한국생화학회지 33 (4): 312~316    
    9. 2002 "N-nitrosoethylurea가 쥐 간세포의 항산화효소의 활성에 미치는 영향" 환경생물 = Korean journal of environmental biology 20 (2): 173~179    
    10. 2002 "Enzymatic Properties of Fast-migrating Cationic Peroxidase Isozyme from Rice Callus" Journal of plant biotechnology 4 (1): 39~44    

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