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Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs

De Graaf, Dirk-C.    (Veterinary and Agrochemical Research Centre   ); Coninck, Hans-De   (Veterinary and Agrochemical Research Centre  ); Petry, Franz   (Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University MainzUU0015010  ); Eeckhout, Ilka-B.    (Veterinary and Agrochemical Research Centre   ); Peeters, Johan-E.    (Veterinary and Agrochemical Research Centre  );
  • 초록

    A Cryptosporidium parvum sporozoite and oocyst λgt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicty of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cruptosporidium immune calves and not by sera from parasite naive animals.


  • 주제어

    Cryptosporidium .   antigen .   gene cloning .   zinc-finger .   bovine.  

  • 참고문헌 (17)

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