본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

토양시료로부터 $\alpha$-glucosidase 저해제 생성 방선균의 분리
Isolation of $\alpha$-glucosiadase Inhibitor Producing Actinomycetes from Soil Sample

하남주   (삼육대학교 약학과UU0000649  ); 최성숙   (삼육의명대학 식품과학과UU0000655  ); 정남용   (삼육의명대학 식품과학과UU0000655  ); 김경제   (삼육대학교 약학과UU0000649  );
  • 초록

    탄수화물분해 효소인 $\alpha$ -glucosidase의 활성을 저해하는 물질을 생성하는 미생물을 검색하기 위하여 토양시료로 부터 수종의 미생물을 검색하였다. 토양 시료에서 강력한 $\alpha$ -glucosidase 저해제를 생성하는 방선균PM718을 분리하였고 그 저해제의 활성을 기지물질인 acarbose와 비교 측정한 결과 우수한 활성을 나타냄을 확인하였다. 또한 그 활성은 pH 변화에도 안정된 활성을 유지하는 것을 확인하였다.


    To find $\alpha$ -glucosidase inhibitors produced by Actinomycetes, bacteria belonging to Actinomycetes were isolated from soil sample using Bennett's medium. The inhibitory activity induced by these bacteria on $\alpha$ -glucosidase, which is the key enzymes far carbohydrates digestion and the prevention of diabetic complications, was investigated. A strain of these bacteria, PM718 potently inhibited $\alpha$ -glucosidase activity in vitro.


  • 주제어

    Actinomycetes .   $\alpha$-glucosidase inhibitor .   carbohydrate digestion.  

  • 참고문헌 (26)

    1. New proteinous inhibitor(Haim) from Streptomyces corichorisii , Murao, S.;N. Oouchi;A. Goto;M. Arai , Agric. Biol. Chem. / v.47,pp.453-454,
    2. α-amylase inhibitor from fungus Cladosporium herbaruum F 828 , Narimaza, S. , J. Biol. Chem. / v.257,pp.3120-4125,
    3. Inhibition of the rate of carbohydrate and lipid absorption by the intestine , Puls, W.;H.P. Krause;L. Muller;H. Schutt;R. Sitt;R.G. Thomas , 4th, Int. Congr. Obesity / v.,pp.,
    4. Isolation of α-amylase inhibitor-producing microorganism , Murao, S.;S. Ogura , Agric. Biol. Chem. / v.41,pp.919-924,
    5. Does a common mechanism induce the diverse complication of diabetes? , Winegrad, A.I. , Diabetes / v.36,pp.396-406,
    6. The effect of short term dietary supplementation with glucose on gastric emptying of glucose and fructose and oral glucose tolerance in normal subject , Horowitz, M.;K.M. Cunningham;J.M. Wishart;K.L. Jones;N.W. Read , Diabetologia. / v.39,pp.481-486,
    7. Enzyme inhibitors , Brodbech, U. , Verlag Chemie / v.,pp.109-112,
    8. Amylostatin, other amylase inhibitors produced by Streptomyces diastaticus , Fukuhara, K.;H. Murai;S. Murao , Agric. Biol. Chem. / v.46,pp.2021-2030,
    9. Assay of intestinal dissacharidase , Dahlqvist, A. , Enzymol. Biol. Clin. / v.11,pp.52-56,
    10. Diabetes and its complications: blood glucose control vs. genetic susceptibility , Rosenstock, J.;P. Raskin , Diab Metab. Rev. / v.4,pp.417-435,
    11. Chemical of an amylase inhibitor, S-AI , Murao, S.;K. Ohyama , Agric. Biol. Chem. / v.43,pp.679-681,
    12. Effects of graded α-glucosidase inhibition on sugar absorption in vivo , Madariaga, H.;P.C. Lee;L.A. Heitlinger;E. Lebenthai , Dig. Dis. Sci. / v.33,pp.1020-1024,
    13. New concepts in the pathogenesis and treatment of noninsulin-dependent diabets mellitus , Denfronzo, R.A.;E. Ferrannini;V. Koivisto , Am. J. Med. / v.17,pp.52-81,
    14. Chemistry and Biochemistry of micobial α-glucosidase inhibitors , Truscheit, F.;W. Fromer;B. Jung;L. Muller;D.D. Schmidt;W. Wingender , Angew. Chem. Int. Ed. Engl. / v.20,pp.744-761,
    15. Hyperglycemia modulates gallbladder motility and small intestinal transit time in man , De Boer, S.Y.;A.A.M. Masclee;W.F. Lam;J. Schipper;J.B.M.J. Jansen;C.B.H.W. Lamers , Dig. Dis. Sci. / v.38,pp.2228-2235,
    16. A new actinomycete species , Yasin, A.F.;E.A. Galinski;A. Wohlfarth;K.D. Jahnke;K.P. Schallz;H.G. Trupper , Int. J. syst. Bacteriol. / v.43,pp.266-271,
    17. Increased disaccharidase activity in human diabetics , Tandon, R.K.;L.M. Srivastav;S.C. Pandey , Am J. Clin Natr / v.28,pp.621-625,
    18. Selective inhibition of histidine-modified pancreatic α-amylase by proteinaceous inhibitor from Phaseolus vulgaris , Nakatani, H. , Arch. Bioche. Biophys. / v.263,pp.364-368,
    19. Puls, W.;Keup, U.;A. Howard(ed.) , Recent advances in obesity resesrch Ⅰ / v.,pp.391,
    20. Carbohydrates digestion and absorption , Gray, G.M. , New Eng. J. Med. / v.292,pp.1225-1230,
    21. Post-prandial glycemic control, hormonal effects and carbohydrate malabsorption during long term adminastration of the α-glucosidase inhibitor miglitol , Lembcke, B.;M. Diedrerich;U.R. Flsch;W. Creutzfeldt , Digestion / v.47,pp.47-55,
    22. Atlas, R.M. , Handbook of microbiological media, Parks, L.C.(1st ed.) / v.,pp.,
    23. Sucrose malabsorption in man after ingestion of α-glucosidehydrolase inhibitor , Caspary, W.F. , Lancet. / v.1,pp.1231-1233,
    24. New proteinous inhibitor(Haim) of animal α-amylase from Streptomyces friceosporeus YM-25 , Murao, S.;A. Goto;Y. Matsuo;K. Ohyama , Agric. Biol. Chem. / v.44,pp.1679-1681,
    25. The secondary metabolism of microorganism: an inexhaustible source for new products , Zahner, H. , Pestic. Sci. / v.16,pp.424-425,
    26. α-glucosidase inhibitors new complex oligosaccharides of microbial orgin , Schmidt, D.D.;B. W-Frommer;L. Junge;W. Muller;E. Truscheit Wingender;D. Schafer , Naturwissenschaften / v.64,pp.535-536,

 저자의 다른 논문

  • Ha, Nam-Joo (59)

    1. 1984 "한국산 Rhizobium 속의 분리 및 동정" Korean journal of microbiology = 미생물학회지 22 (2): 73~76    
    2. 1996 "Characterization of Azomonas agilis PY101, a Cadmium-Resistant Strain Isolated from Anyang Stream" The journal of microbiology 34 (3): 289~293    
    3. 1999 "임상 분리 균주의 항생제 감수성 유형" 약학회지 = Yakhak hoeji 43 (1): 128~130    
    4. 2001 "원유시료에서 분리된 반코마이신 저항성 Coagulase-Negative Staphylococcus 균주의 특성" 약학회지 = Yakhak hoeji 45 (5): 491~493    
    5. 2002 "Isolation, Identification and Characterization of Ynncomycin-Resistant Enterococci from Raw Milk" The journal of microbiology 40 (2): 170~172    
    6. 2002 "The Effect of Linarin on LPS-Induced Cytokine Production and Nitric Oxide Inhibition in Murine Macrophages Cell Line RAW264.7" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 25 (2): 170~177    
    7. 2002 "Activation of Murine Macrophage Cell Line RAW 264.7 by Korean Propolis" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 25 (6): 895~902    
    8. 2003 "Anti-diabetic Effects of CCCA, CMESS, and Cordycepin from Cordyceps militaris and the Immune Responses in Streptozotocin-induced Diabetic Mice" Natural product sciences 9 (4): 291~298    
    9. 2003 "Association Study between Vitamin D Receptor Gene Polymorphism and Adult Periodontitis in Korea" Korean journal of biological sciences 7 (2): 145~149    
    10. 2003 "Two Polymorphisms of Interleukin-4 Gene in Korean Adult Periodontitis" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 26 (6): 482~486    
  • Kim, Kyung-Jae (39)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 한국미생물학회 : 저널
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기