본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

글루탐산 용액 처리에 따른 발아현미 중의 감마-아미노낙산 및 일부 아미노산 함량변화
Changes in the Levels of $\gamma$-Aminobutyric Acid and Some Amino Acids by Application of a Glutamic Acid Solution for the Germination of Brown Rices

오석흥   (우석대학교 생물공학과UU0001001  ); 김수화   (우석대학교 생물공학과UU0001001  ); 문연정   (우석대학교 생물공학과UU0001001  ); 최원규   (우석대학교 생물공학과UU0001001  );
  • 초록

    GABA가 고함유된 발아현미를 생산할 수 있는 전략을 마련하고자 현미발아시 통상적인 물발아구 외에 젖산발아구, glutamic acid발아구로 나누어 발아 이전의 현미와 GABA 및 일부 유리아미노산 함량을 비교분석하였다. 5 mM glutamic acid 용액을 발아에 사용한 경우 가장 높은 GABA 함량 증진을 보여 시료 g당 및 시료 추출물 중의 단백질 mg당 증가정도가 발아하지 않은 현미에 비해 각각 8배와 12배로 나타났다. 또한 glutamic acid 발아구는 물발아나 젖산발아시 현저히 감소되던 serine의 함량을 오히려 증진시켰다. 모든 발아구에서 GABA 및 alanine 함량이 증진된 것과 는 반대로 glutamic acid와 aspartic acid 함량은 현저히 감소 하였다. 이는 발아 과정에 의해 glutamic acid는 GABA로 aspartic acid는 alanine으로 전환된 것에 기인된 것이라 여겨진다. 이상의 결과를 종합하면 현미발아시 glutamic acid액을 사용하면 기능성 물질인 GABA 함량을 현저히 증진시키며, serine의 감소를 막을 수 있어 기능성이 보강된 발아현미를 얻을 수 있을 것으로 기대된다.


    The changes in the levels of $\gamma$ -aminobutyric acid (GABA) and some free amino acids were investigated in germinating brown rices. Ungerminated brown rices were germinated for 72 hrs by application of the following solutions: 1) distilled water, 2) 50 ppm lactic acid, 3) 5 mM glutamic acid. The GABA levels were enhanced in all germinated states of brown rices compared with ungerminated ones, highest in the germinated brown rices by 5 mM glutamic acid solution. Alanine levels were also enhanced significantly in the germinated brown rices. The levels of aspartic acid and glutamic acid were decreased significantly in all the germinated states. The levels of serine decreased during germination in the solutions of water and lactic acid were increased by the germination in the glutamic acid solution. The data show that germination of brown rices by the application of the glutamic acid solution can significantly increase the levels of GABA and can restore the serine level.


  • 주제어

    $\gamma$-aminobutyric acid (GABA) .   amino acids .   brown rices .   germination.  

  • 참고문헌 (20)

    1. The synthesis of γ-aminobutyric acid in response to treatments reducing cytosolic pH , Crawford, L. A.;A. W. Bown;K. E. Breitkreuz;F. C. Guinel , Plant Physiol. / v.104,pp.865-871,
    2. Anaerobic accumulatin of γ-aminobutyric acid and alanine in radish leaves (Raphanus saitivus L.) , Streeter, J. G.;Thompson, J. F. , Plant Physiol. / v.49,pp.572-578,
    3. Effect of anaerobic treatment on carbohydrate-hydrolytic enzyme activities and free amino acid contents in barly malt , Yun, S. J.;K. G. Choi;J. K. Kim , Korean Soci. Crop Sci. / v.43,pp.19-22,
         
    4. Involvement of calcium and calmodulin in protein and amino acids metabolism in rice roots under anoxia , Aurisano, N.;A. Bertani;R. Reggiani , Plant Cell Physiol. / v.36,pp.1525-1529,
    5. Lehninger, A. L.;L. N. David;M. C. Michael , Principles of Biochemistry(2nd ed.) / v.,pp.746,
    6. Bringing the cleft at GABA synapses in the brain , Mody, I.;Y. Dekoninck;T. S. Otis;I. Soltesz , Trends Neurosci. / v.17,pp.517-525,
    7. The metabolism and functions of γ-aminobutyric acid , Bown, A. W.;B. J. Shelp , Plant Physiol. / v.115,pp.1-5,
    8. Metabolism enzymology and possible roles of 4-aminobutyrate in higher plants , Satya Narayan, V.;Nair, P. M. , Phytochemistry / v.29,pp.367-375,
    9. Investigation of γ-aminobutyric acid in Chinese cabbages and effects of the cabbage diets on lipid metabolism and liver function of rats administered with ethanol , Cha, Y. S.;S. H. Oh , J. Korean Soc. Food Sci. Nutr. / v.29,pp.500-505,
         
    10. Calmodulin, calmodulin-related proteins and plant responses to the environment , Snedden, W. A.;H. Fromm , Trends Plant Sci. / v.3,pp.299-304,
    11. Changes in γ-aminobutyric acid(GABA) and the main constituents by treatment conditions and of anaerobically treated green tea leaves , Chang, J. S.;B. S. Lee;Y. G. Kim , Korean J. Food. Sci. Technol. / v.24,pp.315-319,
    12. Accumulation of γ-aminobutyric acid(GABA) in the rice germ , Nakagawa, K.;A. Onota , Food Processing / v.31,pp.43-46,
    13. Cloning and characterization of a tobacco cDNA encoding calcium/calmodulin-dependent glutamate decarboxylase , Yun, S. J.;S. H. Oh , Mol. Cells / v.8,pp.125-129,
    14. Production of the quality germinated brown rices containing high γ-aminobutyric acid by chitosan application , Oh, S. H.;Y. G. Choi , Kor. J. Biotechnol. Bioeng. / v.15,pp.615-620,
         
    15. Application effects of chitosan fertilizer on the growth of cabbage and GABA contents in the cabbage , Oh, S. H.;K. W. Seo;D. S. Choi;K. S. Han , J. Korean Soc. Agric. Chem. Biotechnol. / v.43,pp.34-38,
    16. Analysis of a soluble calmodulin binding protein from fava bean roots: Identification of glutamate decarboxylase as a calmodulin-activated enzyme , Ling, V.;W. A. Snedden;B. J. Shelp;S. M. Assmann , Plant Cell / v.6,pp.1135-1143,
    17. Calcium/calmodulin activation of soybean glutamate decarboxylase , Snedden, W. A.;T. Arazi;H. Fromm;B. J. Shelp , Plant Physiol. / v.108,pp.543-549,
    18. GABA receptors , Krogsgaard-Larsen, P.;M. Williams(ed.);R. A. Glennon(ed.);P.M.W.M. Timmermans(ed.) , Receptor Pharmacology and Function / v.,pp.349-383,
    19. Changes in primary metabolism in connection with alkaloid biosynthesis in solanaceous cell suspension , Marty, D.;F. Mesnard;F. Gillet-Manceau;M. A. Fliniaux;J. P. Monti , Plant Sci. / v.122,pp.11-22,
    20. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle dye binding , Bradford, M. M. , Anal. Biochem. / v.72,pp.248-254,
  • 이 논문을 인용한 문헌 (11)

    1. 2002. "Changes in the levels of Water Soluble protein and flee Amino Acids in Brown Rice Germinated in a Chitosan/Glutamic Acid Solution" 한국생물공학회지 = Korean journal of biotechnology and bioengineering, 17(6): 515~519     
    2. Oh Suk-Heung ; Park Ki-Bum 2004. "Analysis of Plant GABA" Korean journal of crop science = 韓國作物學會誌, 49(suppl1): 73~76     
    3. Jeon, Geon-Uk ; Lee, Mi-Young ; Yoon, Jae-Min ; Jang, Sung-Ho ; Jung, Mi-Ri ; Jeong, Heon-Sang ; Lee, Jun-Soo 2010. "Effects of Heat Treatment and Selected Medicinal Plant Extracts on GABA Content after Germination" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(1): 154~158     
    4. An, Mi-Kyoung ; Ahn, Jun-Bae ; Lee, Sang-Hwa ; Lee, Kwang-Geun 2010. "Anaylsis of ${\gamma}$-aminobutyric Acid (GABA) Content in Germinated Pigmented Rice" 한국식품과학회지 = Korean journal of food science and technology, 42(5): 632~636     
    5. Ko, Jung-A ; Kim, Kyoung-Ok ; Park, Hyun-Jin 2010. "Effects of Molecular Weight and Chitosan Concentration on GABA (${\gamma}$-Aminobutyric Acid) Contents of Germinated Brown Rice" 한국식품과학회지 = Korean journal of food science and technology, 42(6): 688~692     
    6. Moon, Seung-Hee ; Lee, Keun-Bo ; Han, Myung-Kyu 2010. "Comparison of GABA and Vitamin Contents of Germinated Brown Rice Soaked in Different Soaking Solution" 한국식품영양학회지 = The Korean journal of food and nutrition, 23(4): 511~515     
    7. Jo, Seong-Jin ; Hong, Chung-Oui ; Yang, Sung-Yong ; Choi, Kyong-Kun ; Kim, Hyeong-Kook ; Yang, Hyok ; Lee, Kwang-Won 2011. "Changes in Contents of γ-Aminobutyric Acid (GABA) and Isoflavones in Traditional Korean Doenjang by Ripening Periods" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(4): 557~564     
    8. 2011. "" Food science and biotechnology, 20(4): 949~954     
    9. Kim, Ji Myoung ; Yu, Mengying ; Shin, Malshick 2012. "Effect of Mixing Ratio of White and Germinated Brown Rice on the Physicochemical Properties of Extruded Rice Flours" 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 28(6): 813~820     
    10. Oh, Sea-Kwan ; Lee, Jeong-Heui ; Yoon, Mi-Ra ; Kim, Dae-Jung ; Lee, Dong-Hyen ; Choi, Im-Soo ; Lee, Jun-Soo ; Kim, In-Hwan ; Lee, Jeom-Sig 2012. "Physiochemical Properties of Germinated Brown Rice" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(7): 963~969     
    11. Park, Jeong-Seob ; Lee, Kyong-Jin ; Oh, Eun-Bi ; Kim, Hee-Yeon ; Lee, Seung-Yeob ; Choi, Dong-Seong 2014. "Chemical Compositions and Antioxidative Activities of Sweet Potato Foliages Harvested by the Cultivation Period and Tips Location" 한국식품영양학회지 = The Korean journal of food and nutrition, 27(5): 897~905     

 저자의 다른 논문

  • Oh, Suk-Heung (50)

    1. 1995 "Isolation and Characterization of Exogenously Expressed Calmodulin from Endogenous Tobacco Calmodulin by Anion-exchange Fast Protein Liquid Chromatography" Journal of biochemistry and molecular biology = 한국생화학회지 28 (4): 306~310    
    2. 1995 "글리시르히진이 3T3-L1세포의 분화에 미치는 영향" 약학회지 = Yakhak hoeji 39 (5): 535~540    
    3. 1996 "Purification and Characterization of Phytoferritin" Journal of biochemistry and molecular biology = 한국생화학회지 29 (6): 540~544    
    4. 1998 "Carnitine and Calmodulin N-Methylation in Rat Testis; Calmodulin May beInvolved in Carnitine Biosynthesis" Journal of food science and nutrition 3 (3): 251~255    
    5. 1999 "당귀의 첨가 식이가 흰쥐의 지방대사와 알콜대사 및 간기능에 미치는 영향" 한국농화학회지 = Journal of the Korean society of Agricultural Chemistry and Biotechnology 42 (1): 29~33    
    6. 1999 "Transgenic Tobacco Plants Expressing a Mutant VU-4 Calmodulin Have Altered Nicotinamide Co-Enzyme Levels and Hydrogen Peroxide Levels" Journal of biochemistry and molecular biology = 한국생화학회지 32 (1): 1~5    
    7. 1999 "운동 및 고지방식이가 흰쥐의 Carnitine 농도와 carnitine Palmitoyltransferase-I mRNA 수준에 미치는 영향" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 28 (3): 670~676    
    8. 1999 "Effects of Various Calmodulins on the Activation of Glutamate Decarboxylase and Nicotinamide Adenine Dinucleotide Kinase Isolated from Tobacco Plants" Agricultural chemistry & biotechnology 42 (1): 19~24    
    9. 2000 "황련(Coptis chinensis Franch) 추출물의 항충치효과" 한국식품과학회지 = Korean journal of food science and technology 32 (6): 1396~1402    
    10. 2000 "배추의 생장 및 배추 중의 ${\gamma}-aminobutyric$ acid 함량에 미치는 키토산비료의 시비효과" 한국농화학회지 = Journal of the Korean society of Agricultural Chemistry and Biotechnology 43 (1): 34~38    
  • 최원규 (2)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기