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Nutraceuticals and food v.7 no.2, 2002년, pp.128 - 132  

Apoptosis of MCF7 Cells Treated with PKC Inhibitors and Daunorubicin

Park, Won-Chul   (Dept. of Biology, College of Natural Sciences, Pusan National UniversityUU0000613  ); Son, Joo-Young   (Dept. of Biology, College of Natural Sciences, Pusan National UniversityUU0000613  ); Chung, Sook-Hyun   (Dept. of Food Engineering, Dongseo UniversityUU0000470  ); An, Woon-Gun   (Dept. of Biology, College of Natural Sciences, Kyungpook National UniversityUU0000096  );
  • 초록

    The present study was performed to observe the role of protein kinase C (PKC) inhibitors (H-7, staurosporine) and daunorubicin in the cell death process of MCF7 cells; and examined whether or not the type of induced cell death was apoptosis. The usefulness of the combined therapy of PKC inhibitors and daunorubicin to improve the adverse effect of daunorubicin was also investigated. Cell death was induced by treatment with PKC inhibitors or daunorubicin. Characteristic morphologic features of cell shrinkage, chromatic condensation, and cytoplasmic vacuolization were observed. These treatments also stimulated the cleavage of poly-(ADP-ribose) polymerase (PARP), an early event in apoptosis. With slight differences in the percentage of apoptosis-induced cells, staurosporine, H-7 and daunorubicin effectively induced apoptosis in MCF7 cells. Furthermore, combined treatment of PKC inhibitors and daunorubicin significantly drove the cells into an apoptotic state. Hence, our results revealed the possible therapeutic value of combined therapy for the prevention of drug resistance and adverse side effects.


  • 주제어

    apoptosis .   protein kinase C .   MCF7 cells .   daunorubicin.  

  • 참고문헌 (14)

    1. Ronca F, Chan SL, Yu VC. 1997. 1-(5-isoquinolinesulfonyl)-2-methylpiperazine induces apoptosis in human neuroblastoma cells, SH-SY5Y, through a p53-dependent pathway. J Biol Chem 272: 4252-4260 
    2. Shimizu T, Cao CX, Shao RG, Pommier Y. 1998. Lamin B phosphorylation by protein kinase C $\alpha$ and proteolysis during apoptosis in human leukemia HL60 cells. J Biol Chem 273: 8669-8674 
    3. Gooch JL, Yee D. 1999. Strain-specific differences information of apoptotic DNA Ladders in MCF-7 breast cancer cells. Cancer Lett 144: 31-35 
    4. Janicke RU, Sprengart ML, Wati MR, Porter AG. 1998. Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J Biol Chem 273: 9357-9360 
    5. Lopez E, Ferrer I. 2000. Staurosporine- and H-7-induced cell death in SH-SY5Y neuroblastoma cells is associated with caspase-2 and caspase-3 activation, but not with activation of the FAS/FAS-L-caspase-8 signaling pathway. Mol Brain Res 85: 61-66 
    6. Thompson CB. 1995. Apoptosis in the pathogenesis and treatment of disease. Science 267: 456-459 
    7. Harkin ST, Cohen GM, Gescher A. 1998. Modulation of apoptosis in rat thymocytes by analogs of staurosporine- lack of direct association with inhibition of protein kinase C. Mol Pharmacol 54: 663-670 
    8. Ferraris C, Cooklis M, Polakowska RR, Haake AR. 1997. Induction of apoptosis through the PKC pathway in cultured dermal papilla fibroblast Exp Cell Res 234: 37-46 
    9. Bettaieb A, Plo I, Mansat DMV, Quillet MA, Levade T, Laurent G, Jaffrezou JP. 1999. Daunorubicin andmitoxantrone triggered phosphatidylcholine hydrolysis:implication in drug-induced ceramide generation and apoptosis. Mol Pharmacol 55: 118-125 
    10. Garzotto M, Whitejones M, Jiang YW, Ehleiter D, Liaom WC, Haimovitzfriedman A, Fuks Z, Kolesnick R. 1998. 12-O-tetradecanoylphorbol-13-acetate-induced apoptosis in LNCap cells is mediated through ceramide synthase. Cancer Res 58: 2260-2264 
    11. Mansat V, Laurent G, Levade T, Bettaieb A, Zaffrezou JP. 1997. The protein kinase C activators phorbol esters and phosphatidylserine inhibit neural sphingomyelinase activation, ceramide generation, and apoptosis triggered by daunorubicin. Cancer Res 57: 5300-5304 
    12. Otzuka K, Yoshimizu, I. 1998. Visual biochemistry and molecular biology for life science. p 96-99 
    13. Witty JP, Bridgham JY, Johnson AL. 1996. Induction of apoptosis is through the PKC pathway in cultured dermalpapillafibroblast. Endocrinol 137: 5269-5277 
    14. de Silva CP, de Oliveira CR, de Conceicao M, de Lima P. 1996. Apoptosis as a mechnism of cell death induced by different chemotherapeutic drugs in human T-lymphocytes. Biochem Pharmacol 51: 1331-1340 

 저자의 다른 논문

  • Park, Won-Chul (6)

    1. 1985 "SDS Polyacrylamide Gel 電氣泳動에 依한 斧足綱數種의 蛋白質패턴의 比較" 동물학회지 = The Korean journal of zoology 28 (2): 61~70    
    2. 1985 "SDS/polyacrylamide Gel 電氣泳動에 依한 妊娠한 女子 血漿蛋白質의 패턴" 동물학회지 = The Korean journal of zoology 28 (4): 237~244    
    3. 1986 "非姙娠 및 姙娠한 女子의 血淸蛋白質 패턴의 比較" 동물학회지 = The Korean journal of zoology 29 (2): 86~106    
    4. 1986 "SDS/PAGE와 아미노산분석에 의한 논우렁이 [Cipangopaludina chinensis malleata(Reeve)]의 단백질 패턴" 동물학회지 = The Korean journal of zoology 29 (3): 190~200    
    5. 1993 "세포신호계에 있어서 Protein Kinase C: 사람의 전입선 adenocarcinoma PC-3 세포내의 여섯개의 Protein kinase C 동립효소의 translocation" 동물학회지 = The Korean journal of zoology 36 (4): 439~451    
    6. 1999 "Environmental Assessment of Blasting Noise and Vibration in Residential Area" Environmental sciences : bulletin of The Korean Environmental Sciences Society 3 (1): 75~80    
  • Son, Joo-Young (2)

  • 정숙현 (8)

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