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Nutraceuticals and food v.7 no.2, 2002년, pp.184 - 187   피인용횟수: 2

Effect of Gamma-Irradiation on the Molecular Properties of Blood Plasma Proteins

Song, Kyung-Bin   (Department of Food Science and Technology, Chungnam National UniversityUU0001302  ); Lee, Seunghwan   (Department of Food Science and Technology, Chungnam National UniversityUU0001302  ); Lee, Seunghyun   (Department of Food Science and Technology, Chungnam National UniversityUU0001302  );
  • 초록

    Blood products from slaughterhouses that are not hygienically prepared for disposal or food consumption pose a human health hazard. Gamma irradiation is an effective method for sterilization of blood products, but may introduce changes in the molecular characteristics of proteins. This study evaluated the effects of irradiation on animal plasma proteins. Bovine and porcine blood was obtained from a slaughterhouse and the plasma proteins purified and lyophilized. The secondary structure and molecular weight distribution of the plasma protein solutions and powders were examined after ${\gamma}$ -irradiation at 1, 5, 7 and 10 kGy. Gamma-irradiation affected the molecular properties of the protein solutions, but not the protein powders. Circular dichroism and sodium dodecyl sulfate-polyacrylamide gel electrophoresis studies showed that increased doses of ${\gamma}$ -irradiation decrease the ordered structure of plasma proteins in solution, and cause initial fragmentation of the polypeptide chains and subsequent aggregation.


  • 주제어

    plasma proteins .   gamma-irradiation .   molecular properties .   circular dichroism.  

  • 참고문헌 (22)

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    11. Cho Y, Song KB. 1997. Effect of chaotropic salt on the secondary structure of pig skin gelatin. Biosci Biotech Biochem 61: 1194-1195 
    12. Lee MJ, Song KB. 1997. Purification of streptodornase from Streptococcus equisimilis and its DNA-induced conformational change. Biochem Biophys Res Comm 230: 13-15 
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    21. Cho Y, Song KB. 2000. Effect of $\gamma$-irradiation on the molecular properties of BSA and $\beta$-lactoglobulin. J Biochem Mol Biol 33: 133-137 
    22. Moon S, Song KB. 2001. Effect of gamma-irradiation on the molecular properties of ovalbumin and ovomucoid and protection by ascorbic acid. Food Chem 74: 85-89 
  • 이 논문을 인용한 문헌 (2)

    1. 2003. "" Journal of food science and nutrition, 8(4): 343~348     
    2. 2005. "" Journal of food science and nutrition, 10(4): 378~381     

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  • Song, Kyung-Bin (134)

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    9. 1998 "농산물 저장을 위한 키토산코팅 지류 포장재" 한국농화학회지 = Journal of the Korean society of Agricultural Chemistry and Biotechnology 41 (6): 442~446    
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