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개에서 동결수정란의 이식
Embryo Transfer with frozen Embryos in the Dog

김용준   (전북대학교 수의과대학UU0001120  ); 김병진   (동부동물병원HH0064676  ); 유일정   (ACRES연구소  ); 지동범   (지동범동물병원  );
  • 초록

    To investigate the usability of frozen canine embryos for embryo transfer in the dog, 19 donors, 3 recipients, and 6 male dogs were used for the experiment. Natural mating or artificial insemination was performed for breeding the bitches in natural estrus. Vaginal smear test along with progesterone titre test were performed to detect the appropriate mating time and the bitches were bred twice during 3-6days following LH surge. Embryo collection was done on 8, 9-11, 12-13 days after the second mating to collect morula and blastocyst. Embryos were frozen using a programmable freezer and preseued in LNE tank. Embryos were thawed in 37 $^{\circ}C$ water for 15 seconds and transferred into each uterine horn within 30 minutes. Embryos were collected from 13 bitches of 19 donors(68.4%) and the collected embryos were from between 9 and 13 days after 2nd mating. Embryos were produced both by natural mating(60.0%, 9115) and AI with frozen semen(100.0%, 4/4). Embryos were collected from the donors weighed between 2.5 and 30 kg and their age was from 1.5 to 3 years. 52 embryos were collected from 13 donors and the mean number of embryos was four. The stage of embryos was from 2-cell to gastrula and morulae were colledted mostly from 10 to 11 days after 2nd mating. Embryos were collected evenly from each uterine horn and the rate of embryo collection for the number of corpus luteum was 83.9%. Embryos were transferred to 3 recipients(morula 8, blastocyst 1, gastrula 8), however, no offspring was produced.


  • 주제어

    Embryo transfer .   frozen embryos .   morula .   blastocyst .   embryo collection.  

  • 참고문헌 (22)

    1. Surgical collection and transfer of canine embryos , Kinney GM;Pennycook JW;Schriver MD(et al.) , Biol Reprod Suppl / v.1,pp.20.96A,
    2. Embryo transfer in laboratory animals , Kraemer. DC;Bowen MJ;Morrow DA(ed.) , Current therapy in theriogenology 2 / v.,pp.73-78,
    3. Roberts. SJ , Vetrinary obsterics and genital disease(thero-genology) / v.,pp.677-678,
    4. Effects of prostaglandin F2 α on implantation and maintenance of pregnancy in the dog , Tsutsui T;Takatani H;Hirose O(et al.) , Jpn.J.Vet.Sci / v.44,pp.403-410,
    5. Embryo transfer in the non-human primate, feline and canine , Kraemer DC;Flow BI;Schriver MD(et al.) , Theriogenology / v.11,pp.51,
    6. Hormone Levels during the estrous cycle, pregnancy and pseudopregnancy in the Labrador bitch(abstract) , Chakraborty PK;Panko WB;Seager SWJ , Proceedigns of the Amercan Society of Animal Science / v.337,pp.349,
    7. Embryo transfer in dogs and cats , Drost M;Roberts SJ(ed.) , verterinary obstetrics and genital diseases(theriogenology) / v.,pp.938-939,
    8. Lumb WV;Jones EW , Veterinary anesthetia(2nd ed.) / v.,pp.309,
    9. Induction of estrus in bitches with exogenous gonadotropins, and pregnancy rate and blood progesterone profils , Nakao T;Aoto Y;fukushima S(et al.) , Jpn. J. Vet. Sci / v.47,pp.17-24,
    10. Induction of estrus and ovulation in the bitch, using exogenous gonadotropins , Thun R;Watson P;Jackson GL , Am J Vet Res / v.38,pp.483-486,
    11. A surgical method for collecting canine embryos after induction of estrus and ovulation with exogenous gonadotropins , Archbald LF;Baker BA;Clooney LL(et al.) , Vet.Med. Small Anim Clin / v.75,pp.228-238,
    12. Induction estrus in the bitch. , Evans LE;Morrow DA(ed.) , Current therapy in theriogenology / v.,pp.618-620,
    13. Induction of estrus and ovulation in the bitch , Cain JL;Kirk RW(ed.);Bonagura JD(ed.) , Current Veterinary therapy XE small animal pratice / v.,pp.1288-1291,
    14. Efforts to induce estrus in the bitch, using pituitary gonadotropins , Shillie VM;Thatcher MJ;Simmons KJ , JAVMA / v.184,pp.1469-1473,
    15. Use of pulsatile intravenous administration of gonadotropin - releasing hormone to induce fertile estrus in bitches , Cain JL;Cain GR;Feldman EC(et al.) , Am J Vet Res / v.49,pp.1993-1996,
    16. An experimental trial on embryo transfer in the dog , Tsutsui T;Shimada K;Nichi M(et al.) , Jpn.J.Vet.Sci / v.51,pp.797-800,
    17. Embryo transfer in the cat during the non-breeding Season , Tsutsui T;Sato M;Kurosawa N(et al.) , Jpn.J.Vet.Sci / v.51,pp.871-877,
    18. Effects of prostaglandin F2 α -analogue administration on luteal function, implantation of embryos and maintenance of pregnancy in bitches , Tsutsui T;Kasakami E;Orima H(et al.) , Jpn.J.Vet.Sci. / v.51,pp.496-504,
    19. Studies on the reproduction in the dog. A trial of ova transplantation in dogs , Takeishi M;Akai R;Tsunekane T(et al.) , Jpn J Anim Reprod / v.26,pp.151-153,
    20. Feldman EC;Nelson RW , Canine and feline endocrinology and reproductionl / v.,pp.399-413;465-466,
    21. A new method of paternity testing for dog, based on miocrosatellite sequences , Zajc I;Mellersh C;kelly EP;Sampson J , Vet Rec / v.135,pp.545-547,
    22. 자연발정견 및 인공발정 유도견에서 수정란이식 , 김용준 , 대한수의학회지 / v.34,pp.395-406,

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    7. 1993 "개 정자의 수정능력 검정을 위한 Hamster test의 이용 가능성" 韓國臨床獸醫學會誌= Korean journal of veterinary clinical medicine 10 (1): 1~10    
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