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Equex-STM paste 첨가 희석액이 개 정액의 동결.융해 후 정자활력 및 생존율에 미치는 영향
Effects of Semen Extender Containing Equex-STM Paste on Post-thaw Motility and Viability of Canine Sperm

김용준   (전북대학교 수의학과UU0001120  ); 한종현   (전북대학교 수의학과UU0001120  ); 유일정   (ACRES 연구소  ); 지동범   (지동범 동물병원  );
  • 초록

    This study was performed to investigate the freezomg condition especially focused on extender composition to achieve good post-thaw viability and motility of canine sperm. Semen were collected from 6 male dogs which had been proved to be fertile in the past and were treated for freezing. Equex-STM paste was contained in both the 1st(3%) and the 2nd(7%) diluent and the 2nd diluent was added to the 1st diluent following glycerol equilibration for an hour and a half. To investigate the effect of Equex-STM paste in the extender on post-thaw canine sperm characteristics, the post-thaw viability, motility, and HOS(Hypoosmotic swelling) values were evaluated according to the different composition of extender with or without Equex-STM paste, thawing conditions, and different thawing media added to thawed semen. 1. Canine sperm removed from seminal plasma and frozen )n Sweden extender containing Equex showed higher post-thaw viability, motility, and HOS values than those frozen in the extender containing Equex-STM paste with seminal plasma and those frozen in the extender without Equex and seminal plasma. 2. Canine sperm frozen in Sweden extender containing Equex-STM paste with 5% glycerol showed higher post-thaw viability, motility, and HOS values than those frozen with 3%, 8% glycerol or 5% DMSO. 3. The canine semen frozen in Sweden extender with 5% glycerol and Equex-STM paste showed higher viability, motility, and HOS values when thawed at $70^{\circ}C$ for 8 seconds than when thawed at $37.5^{\circ}C$ for 1 min and at $18-20^{\circ}C$ for 5 min. 4. TFC (tris -fructose-citrate) and PB S (phosphate buffered saline) medium added immediately to thawed canine semen brought better viability, motility, and HOS values for the sperm than those semen added with TGC(tris-glucose-citrate) and no medium. These results indicated that Equex-STM paste in Sweden extender for freezing the canine sperm which were removed from seminal plasma brought good post-thaw viability and motility of canine sperm. Also of the freezing conditions of canine sperm with the same extender containing Equex, the concentration of 5% glycerol, the thawing condition at $70^{\circ}C$ for 8 sec, and TFC and PBS medium added to the thawed semen brought better post-thaw viability and motility of canine sperm than the other conditions used in this study.


  • 주제어

    Equex STM paste .   Sweden extender .   frozen canine semen .   thawing viability .   motility.  

  • 참고문헌 (33)

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  • 이 논문을 인용한 문헌 (2)

    1. Yoo Dong-Chul ; Choi Dal-Woong 2004. "Effects of Organic Solvents on Mucus Penetration Distance, Motility and Survival Rate of Human Sperm in vitro" 환경독성학회지 = Journal of environmental toxicology, 19(2): 177~182     
    2. Choi, Sun-Ho ; Kim, Sung-Jae ; Cho, Sang-Rae ; Choe, Chang-Yong ; Son, Jun-Kyu ; Kim, Jong-Suk ; Oh, Suk-Il ; Park, Byung-Jin ; Kim, Sang-Hyun 2010. "Factors Affecting the Survival Rates of Frozen-Thawed Spermatozoa in Jindo Dog by Monosaccharides and Freezing Rate" Journal of embryo transfer = 한국수정란이식학회지, 25(3): 195~199     

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