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한우 및 한국재래산양 유래 말초혈액 림프구의 미소핵을 이용한 방사선 피폭의 생물학적 선량측정
Use of Frequencies of Micronuclei as Biological Dosimetry in Korean Native Cattle and Goat Lymphocytes after Irradiation in vitro

류시윤   (충남대학교 수의과대학UU0001302  ); 김민주   (충남대학교 수의과대학UU0001302  ); 김호준   (충남대학교 수의과대학UU0001302  ); 조성환   (충남대학교 수의과대학UU0001302  ); 김태환   (한국원자력병원  ); 정규식   (경북대학교 수의과대학UU0000096  ); 이해준   (전남대학교 수의과대학UU0001112  ); 김성호   (전남대학교 수의과대학UU0001112  );
  • 초록

    The frequencies of gamma-ray-induced micronuclei (MN) in cytokinesis-blocked (CB) lymphocytes at several doses were measured in three donors of Korean native cattle and Korean native goat. Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was cattle : y : 0.1016D + $0.0118D^2$ +0.0147, goat : y = 0.1353D + $0.0043D^2$ +0.0087 (y : number of MN/CB cells and D = irradiation dose in Gy). The relative sensitivity of goat lymphocytes compared with cattle lymphocytes was estimated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy. In the case of MN frequency with 0.05, 0.1, 0.2, 0.4 and 0.8, the relative sensitivities of goat lymphocytes were 1.106. 1.166. 1.140, 1.069 and 0.976 respectively. Our in vitro radiobiological study confirmed that the cytogenetic response obtained in blood from cattle and goat can be utilized for application in environmental studies.


  • 주제어

    gamma-radiation .   micronuclei .   cattle .   goat .   lymphocyte.  

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