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Nutraceuticals and food v.8 no.1, 2003년, pp.29 - 35   피인용횟수: 1

Characterization of the Immunologically Active Components of Glycyrrhiza uralensis Prepared as Herbal Kimchi

Hwang, Jong-Hyun   (Department of Kimchi and Food Science, Chongju National College of Science and TechnologyUU0001260  ); Lee, Kyong-Haeng   (Department of Kimchi and Food Science, Chongju National College of Science and TechnologyUU0001260  ); Yu, Kwang-Won   (Department of Kimchi and Food Science, Chongju National College of Science and TechnologyUU0001260  );
  • 초록

    A crude polysaccharide fraction (GU-3) from the roots of Glycyrrhiza uralensis (licorice root), a screened herbal plant used in the preparation of herbal kimchi, enhanced Peyer's patch mediated bone marrow cell proliferation and NK cell-mediated tumor cytotoxicity against Yac-1 cells. GU-3 was further purified by DEAE-Sepharose CL-6B yielding fractions designated as GU-3I, and 3IIa∼3IIe. GU-3IIa is mainly composed of arabinose, galactose and galacturonic acid, and showed the highest bone marrow cell proliferation activity. In addition, GU-3IIb had arabinose, galactose, rhamnose and galacturonic acid as the component sugars with a small quantity of protein; GU-3IIb also enhanced activity of NK cell-mediated tumor cytotoxicity. After these fractions were further fractionated via gel filtration on Sepharose CL-6B or Sephacryl S-300, two immunological active polysaccharides, GU-3IIa-2 and 3IIb-1 were purified from the respective fractions. GU-3IIa-2 mostly contained neutral sugars (75%) such as arabinose and galactose (molar ratio; 1.0 : 0.7) in addition to a considerable amount of galacturonic acid (20%), whereas GU-3IIb-1 was composed of arabinose, galactose, rhamnose and galacturonic acid (molar ratio; 0.3 : 0.5 : 0.1 : 1.0). Methylation analysis indicated that GU-3IIa-2 was composed mainly of terminal, 4- or 5-linked and 3,4- or 3,5-branched arabinose, 3-linked, 4-linked and 3,6-branched galactose, and terminal and 4-linked galacturonic acid whereas GU-3IIb-1 contained various glycosidic linkages such as terminal and 4- or 5-linked arabinose, 2,4-branched rhamnose, terminal and 4-linked galactose, and terminal and 4-galacturonic arid. Single radial gel diffusion indicated that only GU-3IIa-2 strongly reacted with β-D-glucosyl-Yariv antigen. These results suggest that bone marrow cell proliferating activity and enhancement of NK cell-mediated tumor cytotoxicity of GU-3 are caused by polysaccharides containing a pectic arabinogalactan (GU-3IIa-2) and pectic polysaccharide (GU-3IIb-1).


  • 주제어

    Clycyrrhiza uralensis .   bone marrow cell proliferation .   NK cell-mediated tumor cytotoxicity .   pectic polysaccharide.  

  • 참고문헌 (25)

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  • 이 논문을 인용한 문헌 (1)

    1. 2004. "Effect of Steam Distillates Prepared from Herbal Medicines on Immunostimulating Activity" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 33(4): 626~632     

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