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Nutraceuticals and food v.8 no.1, 2003년, pp.61 - 65   피인용횟수: 1

Regulation of Acetyl-CoA Carboxylase Gene Expression by Hormones and Nutrients

Kim, Youn-Jung   (Research Institute of Human Ecology, Changwon UniversityUU0001233  ); Yang, Jeong-Lye   (Research Institute of Human Ecology, Changwon UniversityUU0001233  ); Kwun, In-Sook   (Department of Food Science and Nutrition, Andong National UniversityUU0000902  ); Kim, Yang-Ha   (Department of Food and Nutrition, Ewha Womans UniversityUU0001056  );
  • 초록

    This study was investigated to identify the regulatory mechanism of ACC gene expression by hormones and nutrition. The fragment of ACC promoter I (PI) -220 bp region was recombined to pGL3-Basic vector with luciferase as a reporter gene. The primary hepatocyte from the rat was used to investigate the regulation of ACC PI activity. ACC PI (-220 bp)/luciferase chimeric plasmid was transfected into primary rat hepatocyte by using lipofectin. ACC PI activity was shown by measuring luciferase activity. The addition of insulin, dexamethasone, and triiodothyronine to the culture medium increased the activity of ACC PI by 2.5-, 2.3- and 1.8-fold, respectively. In the presence of 1 $\mu$ M dexamethasone, the effects of insulin was amplified about 1.2-fold showing the additional effects of dexamethasone. Moreover the activity of luciferase was increased by insulin, dexamethasone, and triiodothyronine treatment approximately 4-fold. These results indicated that insulin, dexamethasone and thyroid hormone coordinately regulate ACC gene expression via regulation of promoter I activity. On the -220 to +21 region of ACC PI, the addition of the glucose to the culture medium increased the activity of ACC PI. With 25 mM glucose, luciferase activity increased by 7-fold. On the other hand, on the -220 bp region, ACC PI activity was not changed by polyunsaturated fatty acids. Therefore, it can be postulated that there are response elements for insulin, triiodothyronine, dexamethasone, and glucose, but not PUFAs on the -220 bp region of ACC PI.


  • 주제어

    acetyl-CoA carboxylase .   gene expression .   promoter .   primary hepatocytes .   hormone .   nutrition.  

  • 참고문헌 (20)

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  • 이 논문을 인용한 문헌 (1)

    1. Kim, Hyun Sook ; Kim, Tae Woo ; Kim, Dae Jung ; Kim, Kyoung Kon ; Choe, Myeon 2013. "Effects of Medicinal Plant Water Extracts on Expression of Anti-diabetic Enzymes mRNA" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(7): 1008~1014     

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  • Kwun, In-Sook (42)

  • Kim, Yang-Ha (21)

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