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능이버섯(Sarcodon aspratus) 추출물의 생리활성
Physiological Activity of Sarcodon aspratus Extracts

송재환   (성균관대학교 생명공학부UU0000759  ); 이현숙   (성균관대학교 생명공학부UU0000759  ); 황진국   (성균관대학교 생명공학부UU0000759  ); 한정환   (성균관대학교 약학부UU0000759  ); 노정근   (성균관대학교 생명공학부UU0000759  ); 금동혁   (성균관대학교 생명공학부UU0000759  ); 박기문   (성균관대학교 생명공학부UU0000759  );
  • 초록

    천연물 유래 생활습관병의 예방 및 치료에 유용한 성분을 함유하고 있는 소재를 탐색하고자 능이버섯 추출물의 생리활성을 확인하였다. 능이버섯 추출물의 nitrite 제거활성은 pH 1.2에서 강한 활성을 보였으며, 농도 의존적으로 활성이 증가하였고, 0.5 mg/mL 처리 시 저해활성이 99.85 $\pm$ 3.05%로 대조구로 사용한 Vitamin C(0.1 mg/mL)의 99.18 $\pm$ 0.31%과 유사하였다. 전자공여활성 역시 농도 의존적으로 증가하였으며, 0.1 mg/mL에서 90.42 $\pm$ 0.54%로 가장 우수하게 나타났고, 0.05 mg/mL에서도 61.08 $\pm$ 1.38%으로 높게 나타났다. 대조구로 사용한 0.1 mg/mL의 vitamin C는 95.36%, vitamin E는 92.56%, BHT는 49.55로 나타났다. 또한 능이버섯 추출물의 항산화 활성은 0.05 mg/mL 이상의 농도에서 유의차(p $\pm$ 51 unit/g로 나타났으며, 혈전 용해활성은 표준시료인 Streptokinase(제일제당)의 1,180 unit/g보다 높은 1,843.8 unit/g의 활성을 보였고, ACE저해활성은 통상법에서 52.89 $\pm$ 1.93%와 전처리법에서 57.63 $\pm$ 0.61%로 높게 나타났다. 암세포에 대한 능이버섯 추출물의 세포독성에서, 폐암 세포주인 A549에 대한 증식 억제율은 능이버섯 추출물 50 mgg/mL 처리 시 84.71 $\pm$ 2.97%로 가장 높게 나타났고, 자궁암 세포주인 HeLa의 세포독성은 1~50 mgg/mL 농도에서 40% 이상의 증식 억제효과를 나타냈으며, 위암 세포주인 AGS의 경우 5~50 mg/mL에서 약 70%의 높은 증식 억제율을 나타냈다. 간암세포주인 SK- Hep-1에 대한 능이버섯 추출물의 세포독성은 0.1~5 mg/mL까지는 증식 억제율이 증가하였으나 42.53% 수준이었다. Ames test를 이용한 돌연변이원성 실험결과 Salmonella typhimurium TA98 및 TA100의 자연 돌연변이수에 비해 능이버섯 추출물 처리 시 2배 이상의 histidine positive revertant colony가 형성되어 돌연변이 유발성이 있음을 확인하였다.


    This study was carried out to find the preventive medical and therapeutic effects of Sarcodon asparatus on adult disease by employing several biological and biochemical assays. Nitrate scavenging ability(NSA) of Sarcodan asparatus extracts was displayed up to 99.9% at pH 1.2 in a dose-dependent manner. They also had 90.4% electron donating ability(EDA) at the concentration of 0.1 mg/mL. Extracts of Sarcodon asparatus were also able to function as a powerful antioxidant at all concentrations(0.01∼l.0 mg/mL). Furthermore, we observed that 1 mg/mL concentration of the extracts was more powerful than BHT, With respect to fibrolytic activity, Sarcodon asparatus showed 1,843.8 unit/g, which was higher than streptokinase(1,189 unit/g). The inhibitory effects of the extracts on angiotensin converting enzyme, measured by the normal and pretreatment methods, were 53 and 58%, respectively. We also performed cytotoxicity effect of Sarcodon asparatus extracts on a various cancer cell lines. The growth inhibitory effects of the extracts(5.0 mg/mL) on A549, HeLa, AGS, and SK-Hep-1 cells were 78.9, 55.3, 69.0, and 42.5 %, respectively. Interestingly, Sarcodon asparatusextracts induced mutation on Salmonella typhimurium TA98 and TA100 when Ames test was done.


  • 주제어

    Sarcodon aspratus .   fibrinolysis .   antioxldation .   Ames test .   cytotoxicity.  

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  • 이 논문을 인용한 문헌 (10)

    1. Kim, Young-Eon ; Yang, Ji-Won ; Lee, Chang-Ho ; Kwon, Eun-Kyung 2009. "ABTS Radical Scavenging and Anti-Tumor Effects of Tricholoma matsutake Sing. (Pine Mushroom)" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 38(5): 555~560     
    2. Ryu, Jeong-Eun ; Lee, Young-Nam 2009. "Optimal Culture Conditions for MK1 Strain Isolated from Soft-Rotten Tissue of Neungee Mushroom (Sarcodon aspratus) and the Physico-Chemical Properties of the Purified Exopolysaccharide of MK1" Korean journal of microbiology = 미생물학회지, 45(4): 324~331     
    3. Kim, Hyun-Young ; Joung, Eun-Mi ; Hwang, In-Guk ; Jeong, Jae-Hyun ; Yu, Kwang-Won ; Lee, Jun-Soo ; Jeong, Heon-Sang 2010. "Effect of Fermented Ginseng Extract by Mushroom Mycelia on Antiproliferation of Cancer Cells" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(1): 36~41     
    4. Kwon, Sang-Chul 2011. "Biological Activities of Ethanol Extracts from Hericium erinaceus Mycelium on Angelica keiskei and Angelica keiskei Pomace" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(12): 1648~1653     
    5. Kang, Min-Gu ; Bolormaa, Zanabaatar ; Lee, Jong-Seok ; Seo, Geon-Sik ; Lee, Jong-Soo 2011. "Antihypertensive Activity and Anti-gout Activity of Mushroom Sarcodon aspratus" 한국균학회지 = The Korean journal of mycology, 39(1): 53~56     
    6. Cho, Jae-Han ; Park, Hye-Sung ; Han, Jae-Gu ; Lee, Gang-Hyo ; Sung, Gi-Ho ; Jhune, Chang-Sung 2014. "Comparative analysis of anti-oxidant effects and polyphenol contents of the fruiting bodies in oyster mushrooms" Journal of mushrooms = 한국버섯학회지, 12(4): 311~315     
    7. Lee, Hye-Jin ; Do, Jeong-Ryong ; Jung, Sung Keun ; Kim, Hyun-Ku 2014. "Physiological Properties of Sarcodon aspratus Extracts by Ethanol Concentration" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 43(5): 656~660     
    8. Lee, Hye-Jin ; Do, Jeong-Ryong ; Chung, Min-Yu ; Kim, Hyun-Ku 2014. "Antioxidant Activities of Pleurotus cornucopiae Extracts by Extraction Conditions" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 43(6): 836~841     
    9. Lee, Hye-Jin ; Jung, Sung-Keun ; Do, Jeong-Ryong ; Kim, Hyun-Ku 2015. "Optimization of Extraction Conditions of Sarcodon aspratus by Response Surface Methodology" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 44(3): 464~469     
    10. 2015. "" Korean journal for food science of animal resources = 한국축산식품학회지, 35(4): 533~540     

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