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$\beta$-Carotene 첨가식이가 당뇨쥐의 지질과산화물 수준과 항산화효소 활성에 미치는 영향
Effects of $\beta$-Carotene Supplementation on Lipid Peroxide Levels and Antioxidative Enzyme Activities in Diabetic Rats

이완희   (인하대학교 생활과학대학 식품영양학과UU0001092  ); 천종희   (인하대학교 생활과학대학 식품영양학과UU0001092  );
  • 초록

    This study investigated the effect of dietary $\beta$ -carotene supplementation on lipid peroxidation and anti oxidative enzyme activity as indices of oxidative stress in diabetic rats. Fifty Sprague-Dawley male rats aging 7 weeks were used as experimental animals, which were divided into the non-diabetic control group and the diabetic group. The diabetic group received an intraperitoneal injection with streptozotocin to induce diabetes. Then the diabetic rats were divided into four dietary groups which contained different amounts of $\beta$ -carotene; 0%, 0.002%, 0.02%, or 0.2% of the diet. The diabetic rats were fed the experimental diets and the non-diabetic rats were fed the basal diet without $\beta$ -carotene supplementation for 2 weeks and then sacrificed. The diabetic group had a significantly higher blood glucose level than the non-diabetic group. However, blood glucose level were not significantly changed by the level of dietary $\beta$ -carotene supplementation. Compared to the non-diabetic control group, the diabetic control group indicated a significant increase of plasma thiobarbituric acid reactive substance (TBARS). Liver TBARS level also tended to be higher in diabetic control group, although it was not significant. The $\beta$ -carotene supplementation did not reduce plasma TBARS level. However, Liver TBARS level was significantly decreased when 0.02% or more $\beta$ -carotene was supplemented in the diet. The liver lipofuscin level in the diabetic control group was higher than in the non-diabetic control group, but the effect of $\beta$ -carotene supplementation did not show any differences. Superoxide dismutase activity was significantly lower in the diabetic group, but it was increased in groups receiving 0.02% or more $\beta$ -carotene. Compared to the non-diabetic control group, lower activities of catalase and glutathione peroxidase were observed in the diabetic control group, although it was not significant. Catalase and glutathione peroxidase activities tended to increase as the levels of $\beta$ -carotene supplementation increased, although it was not statistically significant. Therefore, it seems that dietary $\beta$ -carotene supplementation might reduce diabetic complications by partly decreasing the lipid peroxidation and increasing the activity of antioxidative enzyme in diabetes.


  • 주제어

    $\beta$-carotene .   diabetes .   lipid peroxides .   antioxidative enzymes.  

  • 참고문헌 (48)

    1. Suh MH, Yang KM, Chang JH. Suh JS. Effect of β-carotene intake on lipid and antioxidative system in diabetic rats. Abstract in fall symposium, p122, The Sac of K. Community Nutrition, 2001  
    2. Increased Oxydative Stress in Sciatic Nerves of Streptozotocin-Induced Diabetic Rat:Lack of Vitamin C Effect , Park,H.S.;Jang,Y.J.;Choi,D.S.;Namgung,M.A.;Lee,Y.J.;Kang,S.A. , Diabetes / v.19,pp.279-286,
    3. Ahn,M.S. , Food and Principles of Food preparation / v.,pp.72,
    4. The role of superoxide anion in the autoxidation of epinephrine and simple assay for superoxide dismutase , Misra,H.P.;Fridovich,I. , J Biol Chem / v.247,pp.3170-3175,
    5. Protective effect of vitamin E, β-carotene and N-acetylcysteine from the brain oxidative stress induced in rats by lipopolysaccharide , Adel,A.;Kheir-Eldin;Tarek,K.M.;Mohamed,Z.G. , IJBCB / v.33,pp.475-482,
    6. Biological antioxidant protection against lipid peroxidation damage , Tapple,A.L. , Am J Clin Nutr / v.23,pp.1137-1139,
    7. CuZn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in lymphocytes and erythrocytes in insulin dependent diabetic children , Bruno,H.;Stefan,L.M.;Gosta,H. , Acta Endocrinologica / v.102,pp.235-239,
    8. Glutathione peroxidase activity in selenium deficient rat liver , Lawrence,R.A.;Burk,R.F. , Biochemical and Biophysical research communications / v.71,pp.952-958,
    9. Glutathione peroxidase and hydroperoxides , Tappel,A.L. , Meth Enzymol / v.52,pp.506-513,
    10. Cho,I.H. , SAS / v.,pp.,
    11. Effect of Dietary Zinc deficiency and age on lipid peroxides and Zinc levels in rat blood and liver , Choi,Y.J.;Chyun,J.H. , Kor J Nutr / v.33,pp.517-523,
         
    12. Changes in In vivo Lipid Peroxidation and Antioxidant Defense System in Streptozotocin-Induced Diabetic Rats:a Time Course Study , Lee,S.Z.;Park,S.H.;Lee,H.S. , Kor J Nutr / v.34,pp.253-264,
         
    13. A study on lipid peroxides and glycosylated serum proteins in KK mice fed vitamin E supplemented diet , Cho,E.J. , Master's Thesis / v.,pp.,
    14. Effects of vitamin E supplementation on lipid peroxidation and glutathione-related antioxidative enzyme in liver and kidney of diabetic KK mice , Hong,M.K. , Master's Thesis / v.,pp.,
    15. β-Carotene affects antioxidant status in non-insulin-dependent diabetes mellitus , Yishai,L.;Haya,Z.;Ami,B.A.;Yoram,K.;Michael,A. , Pathophysiology / v.6,pp.157-161,
    16. Estimation of product of lipid peroxidation (malony dialdehyde) in biochemical systems , Placer,Z.A.;Cushman,L.L.;Johmsom,B.C. , Analytical Biochemistry / v.16,pp.359-364,
    17. Measurement and spectral characteristics of fluorescent pigments in tissues of rats as a function of dietary polyunsaturated fats and vitamin E , Reddy,K.;Fletcher,B.;Tapple,A.L. , J Nutr / v.103,pp.908-915,
    18. Carlberg,I.;Mannervick,B. , Glutathione reductase in methods in enzymology / v.113,pp.484-499,
    19. Effect of antioxidant Vitamins supplementation on Antioxidative status plasma lipid profiles in Korean NIDDM patients , Kang,N.E.;Kim,W.K. , Kor J Nutr / v.32,pp.775-780,
         
    20. Bertram,J.S. , Mechanism of action of β-carotene as a preventive agent in human health In β-carotene and human health / v.,pp.,
    21. Effect of aminoguanidine treatment on the lipid peroxidation and antioxidative enzymes in liver and pancreas of streptozotocin-induced diabetic rats , Kim,K.H. , Mater's Thesis / v.,pp.,
    22. Catalase in vitro , Aebi,H. , Method in Enzymology / v.105,pp.121-126,
    23. Review of the Folin Phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall , Gary,L.;Peterson , Analytical Biochemistry / v.100,pp.201-220,
    24. Effect of intake of exogenous vitamins C,E, β-carotene on the antioxidative status in kidneys of rats streptozotocin-induced diabetes , Mekinova,D.;Chorvathova,V.;Volkovova,K.;Staruchova,M.;Grancicova,E.;Klvanova,J.;Ondreicka,R. , Nahrung / v.39,pp.257-261,
    25. Reduced vitamin E and increased Lipofuscin products in erythrocytes of diabetic rats , Jain,S.K.;Levine,S.N.;Duett,J.;Hollier,B. , Diabetes / v.40,pp.1241-1244,
    26. The activity od erythrocyte antioxidant enzymes in Diabetes Mellitus , Yu,B.J.;Bae,H.Y.;Lee,B.R. , Kor J Int Med / v.44,pp.766-774,
    27. Halliwell,B.;Gutteridge,M.C. , Free radicals in biology and medicine / v.,pp.,
    28. Effect of antioxidant treatment of streptozotocin-induced diabetic rats on endoneurial blood flow, moter nerve conduction velocity, and vascular reactivity of epineurial arterioles of the sciatic nerve , Lawrence,J.C.;Jill,S.G.;Eric,P.D.;Joyce,A.D.;Donald,D.L.;Mark,A.Y. , Diabetes / v.50,pp.1927-1937,
    29. The inhibitory effect of lipid peroxides on the activity of the membrane bound and the solubilized lipoprotein lipase , Wada,K.;Miki,H.;Etoh,M.;Okuda,F.;Kusukawa,R. , Jan Clinc J / v.47,pp.837-842,
    30. Chang,Y.K.;Lee,B.K.;Kim,M.R.;Hwang,G.H. , Clinical Nutrition Hyoil Munwha Sa / v.,pp.,
    31. Antioxidant treatment prevents the development of peripheral nerve dysfunction in streptozotocin-diabetic rat , Cameron,N.E.;Cotter,M.A.;Maxfield,E.K. , Diabetologia / v.36,pp.299-304,
    32. Assessment and analysis related factors to β-carotene Nutritional status in Diabetics and controls , Chung,J.Y. , Master's Thesis / v.,pp.,
    33. Nutritional influence in cellular antioxidant defense system , Ching,K.;Chow , Am J Clin Nutr / v.32,pp.1066-1081,
    34. The Oxidative stress and the Antioxidant system in Type 2 Diabetics with complications , Ha,A.W.;Noh,H.L.;Chung,Y.S.;Lee,K.W.;Kim,H.M.;Cho,J.S. , Diabetes / v.22,pp.253-261,
    35. Measurement of fluorescent lipid peroxidation products in biological systems and tissues , Fletcher,B.L.;Dillard,C.J.;Tapple,A.L. , Analytical Biochemistry / v.52,pp.1-9,
    36. Alteration in free radical tissue defense mechanism in streptozotocin-induced diabetic rats , Saleh,A.W.;David,V.G. , Diabetes / v.36,pp.1014-1018,
    37. Effect of vitamin C on glycosylation of proteins , Davie,S.J.;Gould,B.J.;Yudkin,J.S. , Diabetes / v.41,pp.167-173,
    38. Choi,B.S. , PC-SAS / v.,pp.,
    39. Plasma malonaldehyde levels during myocardial infraction , Dousset,J.C.;Trouilh,M.;Foglietti,M.J. , Clinica Chimica Acta / v.129,pp.319-322,
    40. Liebler,D.C. , Antioxidant reactions of carotenoids In carotenoids in human health, annals of the new york academy of sciences / v.691,pp.20-31,
    41. Role of oxiditive stress in development of complications in diabetes , Baynes,J.W. , Diabetes / v.40,pp.405-412,
    42. Protein glycation and oxidative stress in diabetes mellitus , Wolff,S.P.;Jiang,Z.Y.;Hunt,J.V. , Free Radical Biology and Medicine / v.10,pp.339-352,
    43. Effect of β-carotene intake on lipid and actioxidative system in diabetic rats.Abstract in fall symposium , Suh,M.H.;Yang,K.M.;Chang,J.H.;Suh,J.S. , The Soc of K. Community Nutrition / v.,pp.,
    44. β-Carotene and lipid oxidation , Nedyalka,V.Y.;Kurt,A.;Violeta,G.R. , Fett/Lipid / v.100,pp.444-462,
    45. Antioxidant action of carotenoids , Burton,G.W. , J Nutr / v.119,pp.109-111,
    46. Long-term β-carotene supplementation and risk of type 2 diabetes mellitus: A randomized controlled trial , Liu,S.;Ajani,U.;Chae,C.;Hennekens,C.;Buring,J.E.;Manson,J.E. , JAMA / v.282,pp.1073-1075,
    47. Antioxidative treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity , Morel,D.W.;Chisolm,G.M. , J Lipid Res / v.30,pp.1827-1834,
    48. Pharmacological doses of vitamin E improve insulin action in healthy subjects and non-insulin-dependent diabetes patients , Paolisso,G.;D'Amore,A.;Giugliano,D.;Ceriello,A.;Varricchio,M.;D'Onofrio,F. , Am J Clin Nutr / v.57,pp.650-656,

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