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Bulletin of the Korean Chemical Society v.25 no.11, 2004년, pp.1667 - 1670   피인용횟수: 2

Preparation of Oligonucleotide Arrays with High-Density DNA Deposition and High Hybridization Efficiency

Park, Jeong-Won    (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology   ); Jung, Yong-Won    (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology   ); Jung, Young-Hwan    (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology   ); Seo, Jeong-Sun    (Macrogene, Inc., Department of Biochemistry and Molecular Biology, Seoul National University   ); Lee, Young-Hoon    (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology  );
  • 초록

    In DNA microarray produced by DNA-deposition technology, DNA-immobilization and -hybridization yields on a solid support are most important factors for its accuracy and sensitivity. We have developed a dendrimeric support using silylated aldehyde slides and polyamidoamine (PAMAM) dendrimers. An oligonucleotide array was prepared through a crosslinking between the dendrimeric support and an oligonucleotide. Both DNAimmobilization and -hybridization yields on the solid support increased by the modification with the dendrimers. The increase of the immobilization and hybridization efficiency seems to result from a threedimensional arrangement of the attached oligonucleotide. Therefore, our dendrimeric support may provide a simple and efficient solution to the preparation of DNA microarrays with high-density DNA-deposition and high hybridization efficiency.


  • 주제어

    Dendrimer .   DNA chip .   Hybridization .   Immobilization .   Oligonucleotide.  

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  • 이 논문을 인용한 문헌 (2)

    1. Cha, Seong-Keuck 2005. "" 대한화학회지 = Journal of the Korean Chemical Society, 49(4): 343~348     
    2. 2008. "" Biotechnology and bioprocess engineering, 13(6): 683~689     

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    2. 1997 "5' Processing of RNA I in an Escherichia coli Strain Carrying the rnpA49 Mutation" Journal of biochemistry and molecular biology = 한국생화학회지 30 (2): 162~165    
  • Jung, Young-Hwan (4)

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