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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology v.8 no.6, 2004년, pp.335 - 338   피인용횟수: 1
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[$Ca^{2+}-activated\;K^+$ Currents of Pancreatic Duct Cells in Guinea-pig

Lee, Han-Wook    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Li, Jing Chao    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Koo, Na-Youn    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Piao, Zheng Gen    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Hwang, Sung-Min    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Han, Jae-Woong    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Choi, Han-Saem    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Lee, Jong-Heun    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Kim, Joong-Soo    (Department of Physiology, College of Dentistry, Seoul National University and Dental Research Institute   ); Park, Kyung-Pyo    (Department of Physiology, College of Dentistry  );
  • 초록

    There are numerous studies on transepithelial transports in duct cells including $Cl^-$ and/or $HCO_3^-$ . However, studies on transepithelial $K^+$ transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of $K^+$ currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both $Cl^-$ and $K^+$ conductance were found with KCI rich pipette solutions. When the bath solution was changed to low $Cl^-$ , reversal potentials shifted to the negative side, $-75{\pm}4\;mV$ , suggesting that this current is dominantly selective to $K^+$ . We then characterized this outward rectifying $K^+$ current and examined its $Ca^{2+}$ dependency. The $K^+$ currents were activated by intracellular $Ca^{2+}$ . 100 nM or 500 nM $Ca^{2+}$ in pipette significantly (P $76.8{\pm}7.9\;pA$ , n=4 or $107.9{\pm}35.5\;pA$ , n=6) at +100 mV membrane potential, compared to those with 0 nM $Ca^{2+}$ in pipette $(27.8{\pm}3.7\;pA,\;n=6)$ . We next examined whether this $K^+$ current, recorded with 100 nM $Ca^{2+}$ in pipette, was inhibited by various inhibitors, including $Ba^{2+}$ , TEA and iberiotoxin. The currents were inhibited by $40.4{\pm}%$ (n=3), $87.0{\pm}%$ (n=5) and $82.5{\pm}%$ (n=9) by 1 mM $Ba^{2+}$ , 5 mM TEA and 100 nM iberiotoxin, respectively. Particularly, an almost complete inhibition of the current by 100 nM iberiotoxin further confirmed that this current was activated by intracellular $Ca^{2+}$ . The $K^+$ current may play a role in secretory process, slnce recycling of $K^+$ is critical for the initiation and sustaining of $CI^-$ or $HCO_3^-$ secretion in these cells.


  • 주제어

    Duct cells .   Guinea pig .   Pancreas .   $K^+$ currents .   $Ca^{2+}$.  

  • 참고문헌 (19)

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  • 이 논문을 인용한 문헌 (1)

    1. 2011. "" Journal of Korean dental science, 4(2): 73~78     

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