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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology v.8 no.6, 2004년, pp.301 - 305  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Effects of NMDA, AMPA and Kainate on the Release of Acetylcholine in Rat Hippocampal and Striatal Slices

Kim, Do Kyung    (Department of Oral Physiology, Chosun University College of Dentistry   ); Lee, Se-Oul    (Department of Pharmacology, Wonkwang University School of Medicine   ); Jung, Kyu-Yong    (Department of Pharmacology, Wonkwang University School of Medicine   ); Kim, Jong-Keun    (Deportment of Pharmacology, Chonnam National University Medical School, Chonnum National University Research Institute of Medical Sciences   ); Choi, Bong-Kyu    (Department of Pharmacology, Wonkwang University School of Medicine  );
  • 초록

    This study examined the effects of N-methyl-D-aspartate (NMDA), ${\alpha}-amino$ -3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate on basal and electrically-evoked release of acetylcholine (ACh) from the rat hippocampal and striatal slices which were preincubated with $[^3H]choline$ . Unexpectedly, the basal and evoked ACh release were not affected at all by the treatment with NMDA $(3{\sim}100{\mu}M)$ , AMPA $(1{\sim}100{\mu}M)$ or kainate $(1{\sim}100{\mu}M)$ in hippocampal slices. However, in striatal slices, under the $Mg^{2+}-free$ medium, $30{\mu}M$ NMDA increased the basal ACh release with significant decrease of the electrically-evoked releases. The treatment with $1{\mu}M MK-801 not only reversed the $30{\mu}M$ NMDA-induced decrease of the evoked ACh release, but also attenuated the facilitatory effect of $30\;{\mu}M$ NMDA on the basal ACh release. The treatment with either $30\;{\mu}M$ AMPA or $100\;{\mu}M$ kainate increased the basal ACh release without any effects on the evoked release. The treatment with $10{\mu}M$ NBQX abolished the AMPA- or kainate-induced increase of the basal ACh release. Interestingly, NBQX significantly attenuated the evoked release when it was treated with AMPA, although it did not affect the evoked release alone without AMPA. These observations demonstrate that in hippocampal slices, ionotropic glutamate receptors do not modulate the ACh release in cholinergic terminals, whereas in striatal slices, activations of ionotropic glutamate receptors increase the basal ACh release though NMDA may decrease the electrically-evoked ACh release.


  • 주제어

    NMDA .   AMPA .   Kainate .   Acetylcholine release .   Hippocampus .   Striatum.  

  • 참고문헌 (25)

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