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Natural product sciences v.10 no.6, 2004년, pp.262 - 267  

Validations of Analysis Methods for Decursin and Decursinol Angelate of Angelicae gigantis Radix by Reversed-Phase Liquid Chromatography

Lee, Jong-Pill    (Department of Natural Medicines Evaluation, KFDA   ); Chang, Seung-Yeup    (Department of Natural Medicines Evaluation, KFDA   ); Park, Sang-Yong    (College of Pharmacy, Kyung Hee University  );
  • 초록

    A reversed-phase liquid chromatographic method for decursin and decursinol angelate of Angelicae gigantis Radix, an important crude drug in Korean traditional medicine, was developed and validated. Decursin and decursinol angelate, the structure isomer (pyranocoumarin) each other, are the main organic constituents in Angelicae gigantis Radix. This method was developed using a RP-18 column, UV detector at 280 nm and 50% acetonitrile solution containing 0.01 M sodium dodecyl sulfate and 25 mM sodium dihydrogen phosphate (pH 5.0) as the mobile phase. Various validation parameters were included and evaluated satisfactorily. Linearity was established in range 2-75 mg/ml of decursin and decursinol angelate (correlation coefficient = 0.9997 and 0.9995, respectively). This analytical method showed good accuracy (98.1% and 99.5%, respectively). Precision (repeatability) revealed a relative standard deviation value of 1.71% (decursin) and 3.19% (decursinol angelate). For intermediate precision measure the considered variables were equipment and days. A robustness test showing the influence of deferent counter-ion concentration in mobile phase was also performed.


  • 주제어

    Angelica gigantis Radix .   decursin .   decursinol angelate .   HPLC .   validation.  

  • 참고문헌 (10)

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    3. Ko, W. J. and Ko., I. S., Studies on identification and determination of Angelicae gigantis Radix in pharmaceutical preparations. Bull. K. H. Phanna. Sci., 7, 15-23 (1979) 
    4. Toker, G., Asian, M., Yesilada, E., Memisoglu, M., and Ito, S., Comparative evaluation of the flavonoid content in officinal Tiliae flos and Turkish lime species for quality assessment., BioI. Pham. Bull., 26, 111-121 (2001) 
    5. Chi, H. J., Studies on the coumarin of Umbelliferous plants on Korea (VI)-Chernical components of the root of Angelica gigas NAKAI (II). J. Phann. Soc. Kor. 13,47-50 (1969) 
    6. Ryu, K S., Hong, N. D., Kim, N. J. and Kong, Y Y, Studies on the coumarin constituents of the root of Angelica gigas Nakai.Isolation of decursinol angelate and assay of decursinol angelate and decursin. Kor. J. Pharmacog. 21, 64-68 (1990) 
    7. Yook, C. S. and Kim., T. H., Studies on the determination of decursin in Angelicae gigantis Radix and some Umbelliferae plants. Bull. K. H. Pharmcl. Sci., 18, 133-140 (1990) 
    8. Ryu, K. S. and Yook, C. S., Studies on the coumarins of the root of Angelica gigas NAKAI (I). J. Phann. Soc. Kor. 11, 22-26 (1967) 
    9. Yook, C. S., Park, J. H., Yong, K. S., Lee, S. H., Kim, E. C., Lim, J. P, Moon Y. H., Lee, M. w., Rho, J. S., Bae, K H., Kim, J. W., Park, D. K, and Han, D. R, Modem Phannacognosy, $9^{th}$ Ed, Seoul, 2003, pp. 336-338 
    10. Kang, Y. G., Lee, J. H., Chae, H. J., Kim, D. H., Lee, S. H., andPark, S. Y., HPLC analysis and extraction methods of decursin and decursinol angelate in Angelica gigas root., Kor. J. Pharmacog., 34, 201-205 (2003) 

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