본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Natural product sciences v.10 no.6, 2004년, pp.289 - 295  

Protection of NMDA-Induced Neuronal Cell Damage by Methanol Extract of Myristica Fragrans Seeds in Cultured Rat Cerebellar Granule Cells

Ban, Ju-Yeon    (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University   ); Cho, Soon-Ock    (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University   ); Kim, Ji-Ye    (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University   ); Bang, Kyong-Hwan    (National Institute of Crop Science, RDA   ); Seong, Nak-Sul    (National Institute of Crop Science, RDA   ); Song, Kyung-Sik    (College of Agriculture and Life-Sciences, Kyungpook National University   ); Bae, Ki-Whan    (College of Pharmacy, Chungnam National University   ); Seong, Yeon-Hee    (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University  );
  • 초록

    Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on N-methyl-D-aspartate (NMDA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to $5\;{\mu}g/ml$ , inhibited NMDA (1 mM)- induced neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF $(0.5\;{\mu}g/ml)$ inhibited glutamate release into medium induced by NMDA (1 mM), which was measured by HPLC. Pretreatrnent of MF $(0.5\;{\mu}g/ml)$ inhibited NMDA (1 mM)-induced elevation of cytosolic calcium concentration $([Ca^{2+}]_c)$ , which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents NMDA-induced neuronal cell damage in vitro.


  • 주제어

    Myristica fragrans seed .   Neuroprotection .   NMDA .   Neurotoxicity .   Cerebellar granule cells.  

  • 참고문헌 (43)

    1. Berridge, M.V. and Tan, A.S., Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys., 303, 474-482 (1993) 
    2. Brenner, N., Frank, O.S. and Knight, E., Chronic nutmeg psychosis. J.R. Soc. Med., 86,179-180 (1993) 
    3. Chavko, M., Braisted, J.C. and Harabin, A.L., Effect of MK801 on seizures induced by exposure to hyperbaric oxygen: comparison with AP-7. Toxicol. Appl. Pharmacol., 151, 222-228 (1998) 
    4. Choi, D.W., Glutamate neurotoxicity and disease of nervous system. Neuron 1, 623-634 (1988) 
    5. Choi, D.W., Glutamate neurotoxicity in cortical cell culture is calcium dependent. Neurosci. Lett., 58, 293-297 (1985) 
    6. Chopra, R.N., Nayar, S.L. and Chopra, I.C., Glossary of Indian Medicinal Plants. CSIR, New Delhi, p. 261. (1956) 
    7. Drian, MJ., Kamenka, J.M. and Privat, A, In vitro neuroprotection against glutamate toxicity provided by novel non-competitive Nmethyl- D-aspartate antagonists. J. Neurosci. Res., 57, 927-934 (1999) 
    8. Huang, K.C., The pharmacology of Chinese herbs, CRC press LLC, p. 155, (1999) 
    9. Koh, S.B., Ban, J.Y., Lee, B.Y. and Seong, Y.H., Protective effects of fangchinoline and tetrandrine on hydrogen peroxide-induced oxidative neuronal cell damage in cultured rat cerebellar granule cells. Planta Med., 69, 506-512 (2003) 
    10. Mulsch, A., Busse, R, Mordvintcev, P, Vanin, A., Nielsen, E., Scheel-Kruger, J. and Olesen, S.P, Nitric oxide promotes seizure activity in kainite-treated rats. NeuroReport, 5, 23252328 (1994) 
    11. Nicholls, D.G. and Budd, S.L., Mitochondria and neuronal survival. Physiol. Rev., 80, 315-360 (2000) 
    12. Weiss, J.H., Hartley, D.M., Koh, J. and Choi, D.W, The calcium channel blocker nifedipine attenuates slow excitatory amino acid neurotoxicity. Science 247, 1474-1477 (1990) 
    13. Wong, E.H., Kemp, J.A, Priestley, T., Knight, A.R., Woodruff, G. N. and Iversen, L.L., The anticonvulsants MK-801 is a potent N-methyl-D-aspartate antagonist, Pmc. Natl. Acad. Sci. U. S. A., 83, 7104-7108 (1986) 
    14. Tecoma, E.S., Monyer, H., Goldberg, M.P and Choi, D.W., Traumatic neuronal injury in vitro is attenuated by NMDA antagonists. Neuron 2, 1541-1545 (1989) 
    15. Pereira, C.F. and Oliveira, C.R, Oxidative glutamate toxicity involvesmitochondrial dysfunction and perturbation of intracellular $Ca^{2+}$ homeostasis. Neurosci. Res., 37, 227-236 (2000) 
    16. Merck Index $11^{th}$ Edition; 999-1000 (1989) 
    17. Choi, D.W., Excitotoxic cell death. J. Neurobiol., 23, 1261-1276 (1992). 
    18. Dykens, J.A., Isolated cerebral and cerebellar mitochondria produce free radicals when exposed to elevated $Ca^{2+}$ and $Na^+$ implications for neurodegeneration. J. Neurochem., 63, 584-591 (1994) 
    19. Meldrum, B.S., Excitatory amino acids in epilepsy and potential novel therapies. Epilepsy Res., 12, 189-196 (1992) 
    20. Regan, RF. and Choi, D.W, The effect of NMDA, AMPA/ kainate, and calcium channel antagonists on traumatic cortical neuronal injury in culture. Brain Res., 633, 236-242 (1994) 
    21. Sonavane, G.S., Sarveiya, V.P, Kasture, V.S. and Kasture, S.B., Anxiogenic activity of Myristica fragrans seeds. Pharmacol. Biochem. Behav., 71, 247-252 (2002) 
    22. Van Gils, C. and Cox, P.A., Ethnobotany of nutmeg in the spice islands. J Ethnopharmacol., 42, 117-124 (1994) 
    23. Van Vliet, BJ., Sebben, M., Dumuis, A., Gabrion, J, Bockaert, J. and Pin, J.P, Endogenous amino acid release from cultured cerebellar neuronal cells: Effect of tetanus toxin on glutamate release. J. Neurochem., 52,1229-1230 (1989) 
    24. Bondy, S.C. and Lee, D.K., Oxidative stress induced by glutamate receptor agonists. Brain Res., 610, 229-233 (1993) 
    25. Giusti, P, Franceschini, D., Petrone, D., Manev, H. and Floreani, M., In vitro and in vivo protection against kainate-induced excitotoxicity by melatonin. J. Pineal Res., 20, 226-231 (1996) 
    26. Evans, W.C., Treese and Ev,ms'Pharmcognosy. $14^{th}$ ed. Singapore: Harcourt Brace & Co. Asia, p. 273-275. (1996) 
    27. Nadkami, K.M., Indian Materia Medica vol. 1. Popular Prakashan. Bombay p. 830-834. (1976) 
    28. Solum, D., Hughes, D., Major, M.S. and Parks, T.N., Prevention of normally occurring and deafferentation-induced neuronal death in chick brainstem auditory neurons by periodic blockade of AMPNkainate receptors. J. Neumsci., 17,4744-4751 (1997) 
    29. Bardoul, M., Drian, M.J. and Konig, N., Modulation of intracellular calcium in early neural cells by non-NMDA ionotropic glutamate receptors. Perspect. Dev. Neurobiol., 5, 353-371 (1998) 
    30. Ellison, D.W., Beal, M.F. and Martin, J.B., Amino acid neurotransmitters in postmortem human brain analyzed by high performance liquid chromatography with electro-chemical detection. J. Neurosci., 19, 305-315 (1987) 
    31. Ram, A., Lauria, P, Gupta, R and Sharma, V.N., Hypolipidaemic effect of Myristica fragrans fruit extract in rabbits. J. Eth nopharmacol., 55, 49-53 (1996) 
    32. Bashkatova, V., Narkevich, V., Vitskova, G. and Vanin, A., The influence of anticonvulsant and antioxidant drugs on nitric oxide level and lipid peroxidation in the rat brain during penthylenetetrazole-induced epileptic form model seizures. Prog. Neuro-Psychopharmacol. BioI. Psychiatry, 27, 487-492 (2003) 
    33. Mei, J.M., Chi, W.M., Trump, B.F. and Eccles, C.U., Involvement of nitric oxide in the deregulation of cytosolic calcium in cerebellar neurons during combined glucose-oxygen deprivation. Mol. Chem. Neuropathol., 27,155-166 (1996) 
    34. Whit, R.J. and Reynolds, I.J., Mitochondrial depolarization in glutamate-stimulated neurons: an early signal specific to excitotoxic exposure. J. Neurosci., 16, 5688-5697 (1996) 
    35. Larm, J.A., Beart, P.M. and Cheung, N.S., Neurotoxin domoic acid produces cytotoxicity via kainate- and AMPA-sensitive receptors in cultured cortical neurons. Neurochem. Int., 31, 677-682 (1997) 
    36. Lesort, M., Esclaire, F., Yardin, C. and Hugon, J., NMDA induces apoptosis and necrosis in neuronal cultures. Increased APP immunoreactivity is linked to apoptotic cells. Neurosci. Lett., 221,213-216 (1997) 
    37. McNamara, J.O., Russell, R.D., Rigsbee, L. and Bonhaus, D.W., Anticonvulsant and antiepileptogenic actions of MK-801 in the kindling and electroshock models. Neurophannacol. 27, 563568 (1988) 
    38. Mody, I. and MacDonald, J.F., NMDA receptor-dependent excitotoxicity: the role of intracellular $Ca^{2+}$ release. Trends Pharmacol. Sci., 16, 356-360 (1995) 
    39. Baltrons, M.A., Saadoun, S., Agullo, L. and Garcia, A., Regulation by calcium of the nitric oxide/cyclic GMP system in cerebellar granule cells and astroglia in culture. J. Neurosci. Res., 49, 333-341 (1997) 
    40. Gunasekar, P.G., Sun, Pw., Kanthasamy, A.G., Borowitz, J.L., and Isom, G.E., Cyanide-induced neurotoxicity involves nitric oxide and reactive oxygen species generation after N-MethylD- aspartate receptor activation. J. Phannacol. Exp. Ther., 277, 150-155, (1996) 
    41. Jensen, J.B., Schousboe, A. and Pickering, D.S., AMPA receptor mediated excitotoxicity in neocortical neurons is developmentally regulated and dependent upon receptor desensitization. Neurochem. Int., 32, 505-513 (1998) 
    42. Rothman, S.M. and Olney, J.W, Glutamate and the phathophy siology of hypoxic-ischemic brain damage. Ann. Neurol., 19, 105-111 (1986) 
    43. Duffy, S. and MacViar, B.A, In vitro ischemia promotes calcium influx and intracellular calcium release in hippocampal astrocytes. J. Neurosci., 16, 71-81 (1996) 

 저자의 다른 논문

  • 반주연 (6)

    1. 2003 "Crude Extract of Zizyphi Jujube Semen Protects Kainic Acid-induced Excitotoxicity in Cultured Rat Neuronal Cells" Natural product sciences 9 (4): 249~255    
    2. 2003 "Methanol Extract of Polygalae Radix Protects Excitotoxicity in Cultured Neuronal Cells" 韓國藥用作物學會誌 = Korean journal of medicinal crop science 11 (4): 298~305    
    3. 2003 "General Pharmacology of ADP" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 11 (1): 51~57    
    4. 2005 "Protective Effect of Sanguisorba officinalis L. Root on Amyloid ${\beta}$ Protein (25-35)-induced Neuronal Cell Damage in Cultured Rat Cortical Neuron" 韓國藥用作物學會誌 = Korean journal of medicinal crop science 13 (5): 219~226    
    5. 2006 "Methanol Extract of Paeonia Japonica Root Protects Cultured Rat Cortical Neurons Against Oxidative Damage Induced by Hydrogen Peroxide" 韓國藥用作物學會誌 = Korean journal of medicinal crop science 14 (2): 70~76    
    6. 2007 "과산화수소수로 유도된 배양신경세포손상에 대한 참죽나무잎 추출물의 보호효과" 韓國藥用作物學會誌 = Korean journal of medicinal crop science 15 (6): 444~450    
  • 조순옥 (6)

  • Kim, Ji-Ye (4)

  • Song, Kyung-Sik (75)

  • Bae, Ki-Hwan (166)

  • Seong, yeon-Hee (42)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기