본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Natural product sciences v.10 no.6, 2004년, pp.347 - 352  

Biotransformation of Ginseng Extract to Cytotoxic Compound K and Ginsenoside $Rh_2$ by Human Intestinal Bacteria

Bae, Eun-Ah    (College of Pharmacy, Kyung Hee University   ); Choo, Min-Kyung    (College of Pharmacy, Kyung Hee University   ); Lee, Young-Churl    (Korea Food Research Institute   ); Kim, Dong-Hyun    (College of Pharmacy, Kyung Hee University,East-West Medical Research Institute, Kyung Hee University  );
  • 초록

    When saponin extracts of dried ginseng and red ginseng were anaerobically incubated with human intestinal microflora, these extracts were metabolized to compound K and ginsenoside $Rh_2$ , respectively. However, when these extracts were incubated with commercial lactic acid bacteria, these did not metabolize these ginsenosides to compound K or ginsenoside $Rh_2$ . Among some intestinal bacteria isolated from human feces, Bacteroides C-35 and C-36 transformed these saponin extracts to compound K and ginsenoside $Rh_2$ , respectively. These bacteria also transformed water extracts of dried ginseng and red ginseng to compound K and ginsenoside $Rh_2$ , respectively, similarly with that of the saponin extracts. Among transformed ginsenosides, compound K and 20(S)-ginsenoside $Rh_2$ exhibited the most potent cyotoxicity against tumor cells.


  • 주제어

    ginseng .   intestinal bacteria .   transformation .   compound K .   ginsenoside $Rh_2$ .   cytotoxicity.  

  • 참고문헌 (20)

    1. Akao, T., Kanaoka, M., Kobashi, K, Appearance of compound K, a major metbolite of ginsenoside Rb1 by intestinal bacteria, in rat plasma after oral aministrationmeasruement of compound K by enzyme immunoassay. Biol. Pharm. Bull., 21, 245-249 (1998a) 
    2. Bae, E.A, Park, S.Y., Kim, D.H., Constitutive $\beta$-glucosidases hydrolyzing ginsenoside RbI and Rb2 from human intestinal bacteria. Biol. Pharm. Bull., 23, 1481-1485 (2000) 
    3. Carmichael, J., DeGreff, W.G., Gazdar, A.F., Minna, J.D., Mitchell, J.B., Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chmosensitivity testing. Cancer Res., 47,936-940 (1987) 
    4. Han, B.H., Park, M.H., Han, Y.N., Woo, L.K, Sankawa, D., Yahara, S., Tanaka, O., Degradation of ginseng saponins under mild acidic conditions. Planta Med., 44, 146-149 (1982) 
    5. Hasegawa, H., Sung, J.H., Benno, Y., Role of human intestinal Prevotella oris in hydrolyzing Ginseng saponis. Planta Med., 63,436-440 (1997) 
    6. Karikura M., Miyaze T., Tanizawa H., Taniyzma T., Takino Y., Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VII. Comparison of the decomposition modes of ginsenoside Rb1 and Rb2 in the digestive tract of rats. Chem. Pharm. Bull., 39, 2357-2361 (1991) 
    7. Lee S.J, Sung J.H., Lee S.J., Moon C.K, Lee B.H., Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin. Cancer Lett., 144,39-43 (1999). 
    8. Shibata, S., Fujita, M., Itokawa, H., Tanaka, O., Ishii, T., Panaxadiol, a sapongenin of ginseng roots (1). Chern. Pharm. Bull., 11, 759-764 (1963) 
    9. Tanaka N., Tanaka O., Shibata S., Chemical studies on the oriental plant drugs. XXVIII. Saponins and sapogenins of ginseng; Stereochemistry of sapogenin of ginsenoside Rb 1, Rb2 and Rc. Chem. Pharm. Bull., 20, 1212-1216 (1972) 
    10. Han, B.H., Park, M.H., Han, Y.N., Woo, L.K., Sankawa, D., Yahara, S., Tanaka, O., Degradation of ginseng saponins under mild acidic conditions. Planta Med., 44, 146-149 (1982) 
    11. Holdeman LV, Kelley RW, and Moore WEC Genus Bacteroides, In Krieg N.R., and lG. Holt (ed.) Bergeys manual of systemic bacteriology. Williams and Wilkins, Baltimore, pp 602-631 (1984) 
    12. Mochizuki et al. Mochizuki M, Yoo CY, Matsuzawa K, Sato K, Saiki I, Tono-oka S, Samukawa K, Azuma I. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside Rb2, 20(R)- and 20(S)-ginsenoside Rg3, of Red ginseng. Biol. Pharm. Bull., 18, 1197-1202 (1995) 
    13. Akao, T., Kida, H., Kanaoka, M., Hattori, M., Kobashi, K, Intestinal bacterial hydrolysis is required for the appearance of compound K in rat plasma after oral administration of ginsenoside RbI from Panax ginseng. J. Pharm. Pharmacal., 50, 1155-1160 (1998b) 
    14. Kanaoka, M, Kato, H, Shimada, F., Yano, S., Studies on the enzyme immunoassay of bioactive constituents contained in oriental medicinal drugs. VI. Enzyme immunoassay of ginesenoside RbI form Panax ginseng. Chem. Pharol. Bull., 40,314-317 (1992) 
    15. Wakabayashi C., Hasegawa H., Murata J., Saiki I., In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol. Res., 9, 411-417 (1998) 
    16. Kitagawa, I., Yoshikawa, M., Yoshihara, M., Hayashi, T., Taniyama, T., Chemical studies on crude drug precession. I. On the constituents of ginseng radix rubura (I). Yakugaku Zasshi, 103,612-622 (1983) 
    17. Bae, E.A, Han, M.J., Choo, M.K., Park, S.Y., Kim, D.H., Metabolism of 20(S)- and 20(R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activities. Bioi. Pharm. Bull., 25, 58-63 (2002) 
    18. Kanaoka, M., Akao, T., Kobashi, K, Metabolism of ginseng saponins, ginsenosides, by human intestinal bacteria. J. Tradit. Med., 11, 241-245 (1994) 
    19. Wu J.Y., Gardner B.H., Murphy Cr., Seals J.R., Kensil CR., Recchia l, Beltz G.A, Newman G.W., Newman M.J., Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-l vaccine. J. Immunol., 148, 1519-1525 (1992) 
    20. Sato K, Mochizuki M., Saiki I., Yoo Y.C, Samukawa K, Azuma I., Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng-ginsenoside Rb2.Biol. Pharm. Bull., 17,635-639 (1994) 

 저자의 다른 논문

  • 배은아 (32)

    1. 1994 "대두유, 면실유 및 미강유로 튀긴약과의 저장성에 관한 연구" 韓國食生活文化學會誌 = Journal of the Korean Society of Food Culture 9 (4): 335~340    
    2. 1995 "Induction and Inhibition of Iindole Production of Intestinal Bacteria" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 18 (5): 351~355    
    3. 1996 "소비자들의 식품선택기준과 기능성 식품에 대한 인식도" 韓國食生活文化學會誌 = Journal of the Korean Society of Food Culture 11 (3): 299~304    
    4. 1996 "버섯의 장내 유산균 증식 효과" 한국식품과학회지 = Korean journal of food science and technology 28 (5): 947~952    
    5. 1998 "Hesperidin 대사에 의한 대금음자(對金飮子)와 평위산(平胃散)의 처방해석" 생약학회지 29 (2): 136~141    
    6. 1998 "재배상황버섯의 장내 세균 유해효소 및 알파글루코시다제 저해효과" 한국식품위생안전성학회지 = Journal of food hygiene and safety 13 (1): 20~23    
    7. 1999 "감초 함유 처방의 글리치리진 대사와 몇가지 효소저해효과" 생약학회지 30 (3): 269~274    
    8. 1999 "Bacillus licheniformis AJ 균주제제의 설사원인 미생물의 성장 억제효과" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 7 (4): 385~389    
    9. 2000 "Scientific Analysis of the Formulation Theory of Chungpesagan-tang; In Vitro Cytotoxicity of Chungpesagan-tang" Natural product sciences 6 (1): 25~30    
    10. 2000 "Scientific Analysis of Formulation Theory of Chungpesagan-tang; In vitro Cytotoxicity of Cisplatin Combined with Chungpesagan-tang" Natural product sciences 6 (4): 165~169    
  • Choo, Min-Kyung (2)

  • 이영철 (53)

  • Kim, Dong-Hyun (194)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기