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액체배양한 느타리 버섯균(Pleurotus ostreatus)으로부터 망간퍼옥시데이즈의 생산 및 특성
Production and Characterization of Manganese Peroxidase from the White Rot Fungus Pleurotus ostreatus in Liquid Culture

이재성   (영남대학교 자연자원대학 생물자원공학부 식품가공학과UU0000951  ); 하효철   (풀무원 식문화 연구원  );
  • 초록

    리그닌 분해균인 느타리 버섯균(Pleurotus ostreatus) K-2946을 glucose-peptone-yeast(G-P-Y) 액체배지에서 배양 하여 망간퍼옥시데이즈를 생산하였다. 본 실험조건 하에서 리그닌 퍼옥시데이즈는 생산되지 않았다. 느타리버섯균 K-2946이 생산하는 망간 퍼옥시데이즈를 이온크로마토그래피, 겔크로마토그래피를 이용하여 정제하였다. 정제한 망간 퍼옥시데이즈의 분자량은 36400 Da이였고 pI는 3.95였다. 정제된 효소의 최적 pH는 5.0이었으며 최적온도는 $55^{\circ}C$ 이었다.


    The ligninolytic basidiomycete, Pleurotus ostreatus K-2946, was produced a manganese peroxidase (MnP) activity when grown in liquid culture with glucose-yeast-peptone (G-Y-P) medium. However, lignin peroxidase (LiP) was not detected in this culture medium. The purification progress of MnP was purified that included chromatography on Sepharose CL-6B, Superdex 75 prep grade and Mono-Q. MnP purified by column chromatography, was 36400 dalton and a pI of 3.95. The optimal pH and temperature of the purified MnP activity were 5.0 and $55^{\circ}C$ . The characteristics of MnP produced was quite similar to those of MnP 3 isoenzyme produced by other strains of P. ostreatus.


  • 주제어

    느타리버섯 .   망간퍼옥시데이즈 .   액체배양.  

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