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거대배아미 에탄올 추출물의 항산화활성 및 항변이원성
Antioxidative and Antimutagenic Activity of Ethanolic Extracts from Giant Embroynic Rices

강미영   (경북대학교 식품영양학과UU0000096  ); 이연리   (경북대학교 식품영양학과UU0000096  ); 고희종   (서울대학교 농학과UU0000691  ); 남석현   (아주대학교 생명과학과UU0000892  );
  • 초록

    신선찰거대배아미, 화청거대배아미 및 남풍거대배아미 등 거대배 돌연변이 계통 쌀 3종류 및 일반미의 70% 에탄올 추출물을 제조하여, 이들의 항산화 활성 및 항변이원성을 비교 검정하였다. 거대배아미 추출물의 항산화 활성은 DPPH radical 및 Fenton 반응에 의해서 유도되는 hydroxy radical의 소거활성, hypoxanthine/xanthine oxidase system에서 생성되는 활성산소종인 superoxide radical의 소거활성, linoleic acid자동산화에 대한 지질 과산화 억제활성 및 토끼 적혈구 막지질의 과산화 억제활성 둥으로써 검정하였으며, 항변이원성은 E. coli PQ 37 균주를 사용하여 화학적 변이원 mitomycin C에 대한 변이원성 억제효과를 SOS chromotest에 의해서 검정하였다. 일반미 품종에 비해서 거대배아미 품종의 항산화 활성 및 항변이원성이 모두 높았으며, 거대배아미 품종 중에서는 남풍거대배아미가 가장 효과적인 경향이 있었다. 남풍거대배아미의 DPPH radical, superoxide radical과 hydroxyl radical 소거활성, 그리고 지질과산화 억제활성은 일반미보다 각각 2.3배, 3.3배, 1.7배 및 2.5배 정도까지 더 높았다.


    70% ethanolic extracts were prepared from the three mutant rice cultivars with giant embryo termed Shinsunchal-giant embryonic rice, Whachung-giant embryonic rice and Nampung-giant embryonic rice, and its antioxidative and antimutagenic properties were evaluated and compared. For analysing antioxidativity, various antioxidative indices, such as electron donating ability to DPPH radical, scavenging capacity to hydroxyl radicals generated by Fenton reaction, scavenging capacity to superoxide radicals generated by HPX/XOD system, inhibitory effect on autoxidation of linoleic acid and inhibitory effect on membrane lipid peroxidation derived from rabbit erythrocyte ghost, were determined. For analysing antimutagenicity, suppressive effects on mutagenesis induced by the chemical mutagen, mitomycin C, were measured using E. coli PQ 37 as a indicator cell. The results showed that for both antioxidativity and antimutagenicity the giant embryonic rices were more effective compared to the general cooking rice, Among the giant embryonic rice cultivars, Nampung-giant embryonic rice tended to be most effective, showing its scavenging activity to DPPH radical, superoxide radical and hydroxyl radical, and inhibitory activity to lipid peroxidation was 2,3-, 3,3-, 1.7-, and 2.5-fold greater than those of normal rice, respectively.


  • 주제어

    거대배아미 .   항산화성 .   항변이원성 .   지질과산화 억제.  

  • 참고문헌 (20)

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  • 이 논문을 인용한 문헌 (7)

    1. Lee, Youn-Ri ; Nam, Seok-Hyun ; Kang, Mi-Young 2006. "Hypoglycemic Effect of the Giant Embryonic Rice Supplementation on Streptozotocin-induced Diabetic Rats" 한국식품과학회지 = Korean journal of food science and technology, 38(3): 427~431     
    2. Lee, Youn-Ri ; Kim, Chae-Eun ; Nam, Seok-Hyun ; Kang, Mi-Young 2006. "Supplementary Effect of the Giant Embryonic Rice on Serum and Heaptic Lipid Levels of Streptozotocin-induced Diabetic Rats" 한국식품과학회지 = Korean journal of food science and technology, 38(4): 562~566     
    3. Seo, Sun-Jung ; Choi, Young-Min ; Lee, Sun-Mi ; Kim, Kee-Jong ; Son, Jong-Rok ; Lee, Jun-Soo 2007. "Determination of Selected Antioxidant Compounds in Specialty Rice" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 36(4): 499~502     
    4. Seo, Sun-Jung ; Choi, Young-Min ; Lee, Seon-Mi ; Kong, Su-Hyun ; Lee, Jun-Soo 2008. "Antioxidant Activities and Antioxidant Compounds of Some Specialty Rices" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 37(2): 129~135     
    5. Woo, Koan-Sik ; Jeong, Eung-Gi ; Suh, Sae-Jung ; Yang, Chang-Ihn ; Jeong, Heon-Sang ; Kim, Kee-Jong 2008. "Antioxidant Components and Antioxidant Activities of 70% Ethanol Extracts on Suweon-511 and Ilpum Rice" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 37(10): 1223~1230     
    6. Woo, Koan-Sik ; Chun, A-Reum ; Oh, Sea-Kwan ; Kim, Kee-Jong ; Kim, Dae-Jung ; Yang, Chang-Ihn ; Kim, Yeon-Gyu ; Kim, Jae-Hyun ; Jeong, Heon-Sang 2010. "Antioxidant and Antitumor Activities of Ethanol Extracts from Unhulled and Hulled Rice Hiami (Oryza sativa L. cv. Hiami)" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(2): 179~185     
    7. Kim, Dae-Jung ; Oh, Sea-Kwan ; Yoon, Mi-Ra ; Chun, A-Reum ; Hong, Ha-Cheol ; Lee, Jun-Soo ; Kim, Yeon-Kyu 2010. "Antioxidant Compounds and Antioxidant Activities of the 70% Ethanol Extracts from Brown and Milled Rice by Cultivar" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(3): 467~473     

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