본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology v.12 no.4, 2004년, pp.228 - 234  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Hydrogen Peroxide Mediates Brazilin-induced Glucose Transport in Adipocytes

Khil, Lee-Yong    (College of Pharmacy, Seoul National University   ); Moon, Chang-Kiu    (College of Pharmacy, Seoul National University  );
  • 초록

    Brazilin shows hypoglycemic effect in diabetic animals through enhancement of glucose metabolisms in insulin responsive tissues. One of the major mechanisms of brazilin to enhance glucose metabolism is stimulation of glucose transport in adipocytes. In this study, the essential molecular moiety of brazilin for the stimulation of glucose transport was investigated. We found that brazilin undergoes a structural change in physiological buffer and produces hydrogen peroxide. Methylation of hydroxyl group of brazilin or addition of catalase along with brazilin resulted in the complete inhibition of brazilin-induced glucose transport in adipocytes. Because hydrogen peroxide increases glucose transport by inhibition of phosphatases, we examined the effect of brazilin on phosphatase activity. Brazilin inhibited phosphatases in a wide range of activity, and protein phosphatase 1 and 2A were also inhibited. These results suggest that the production of hydrogen peroxide by oxidation of catechol hydroxyl group of brazilin mediates glucose transport through inhibition of phosphatases which otherwise decrease glucose transport in adipocytes.


  • 주제어

    brazilin .   hydrogen peroxide .   glucose transport .   adipocyte .   phosphatase.  

  • 참고문헌 (26)

    1. Bialojan, C. and Takai, A. (1988). Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem. J. 256, 283-290 
    2. Bracke, M.E., De Pestel, G., Castronovo, V., Vyncke, B., Foidart, J.M., Vakaet, L.C. and Mareel, M.M. (1988). Flavonoids inhibit malignant tumor invasion in vitro. Prog. Clin. BioI. Res. 280, 219-233 
    3. Cohen, P., Alemany, S., Hemmings, B.A., Resink, T.J., Stralfors, P. and Tung, H.Y. (1988). Protein phosphatase-l and protein phosphatase-2A from rabbit skeletal muscle. Methods Enzymol. 159, 390-408 
    4. DeFronzo, R.A., Bonadonna, R.C. and Ferrannini, E. (1992). Pathogenesis of NIDDM. A balanced overview. Diabetes Care. 15,318-368 
    5. DeFronzo, R.A., Gunnarsson, R., Bjorkman, O., Olsson, M. and Wahren, J. (1985). Effects of insulin on peripheral and splanchnic glucose metabolism in noninsulin-dependent (type II) diabetes mellitus. J. Clin. Invest. 76, 149-155 
    6. Fidelus, R.K. (1988). The generation of oxygen radicals: a positive signal for lymphocyte activation. Cell. Immunol. 113, 175-182 
    7. Green, A. (1986). The insulin-like effect of sodium vanadate on adipocyte glucose transport is mediated at a post-insulinreceptor level. Biochem. J. 238, 663-669 
    8. Halliwell, B. and Gutteridge, J.M.C. (1988). Free radicals in biology and medicine. Oxford Press., UK 
    9. Hikino, H., Taguchi, T., Fujimura, H. and Hiramatsu, Y. (1977). The validity of oriental medicine. Planta Med. 31, 214-220 
    10. James, D.E. and Piper, R.C. (1994). Insulin resistance, diabetes, and the insulin-regulated trafficking of GLUT-4. J. Cell BioI. 126, 1123-1126, 1994 
    11. Jun, H., Bae, H.Y., Lee, B.R., Koh, K.S., Kim, Y.S., Lee, K.W., Kim, H. and Yoon, J. (1999). Pathogenesis of non-insulindependent (type m diabetes mellitus (NIDDM)-genetic predisposition and metabolic abnormalities. Adv. Drug Deliv. Rev. 35, 157-177 
    12. Khil, L.Y, Han, S.S., Kim, S.G., Chang, T.S., Jeon, S.D., So, D.S. and Moon, C.K. (1999). Effects of brazilin on GLUT4 recruitment in isolated rat epididymal adipocytes. Biochem. Pharmacol. 58, 1705-1712 
    13. Koshio, O., Akanuma, Y. and Kasuga, M. (1988). Hydrogen peroxide stimulates tyrosine phosphorylation of the insulin receptor and its tyrosine kinase activity in intact cells. Biochem. J. 250, 95-101 
    14. Kruszynska, Y.T. and Olefsky, J.M. (1996). Cellular and molecular mechanisms of non-insulin dependent diabetes mellitus. J. Investig. Med. 44,413-428 
    15. Mahadev, K., Zilbering, A., Zhu, L. and Goldstein, B.J. (2001). Insulin-stimulated hydrogen peroxide reversibly inhibits protein-tyrosine phosphatase 1b in vivo and enhances the early insulin action cascade. J. Biol. Chem. 276, 21938-21942 
    16. Martin, J.P. Jr, Dailey, M. and Sugarman, E. (1987). Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch. Biochem. Biophys. 255, 329-336 
    17. Masaki, H., Atsumi, T. and Sakurai, H. (1995). Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem. Biophys. Res. Commun. 206, 474-479 
    18. Moon, C.K., Lee, S.H., Chung, J.H., Won, H.S., Kim, J.Y, Khil, L.Y., et al. (1990). Effects of brazilin on glucose metabolism in isolated soleus muscles from streptozotocin induced diabetic rats. Arch. Pharm. Res. 13, 359-364 
    19. Moon, C.K., Lee, S.H., Lee, M.O. and Kim, S.G. (1993). Effects of Brazilin on glucose oxidation, lipogenesis and therein involved enzymes in adipose tissues from diabetic KK-mice. Life Sci. 53, 1291-1297 
    20. Moon, C.K., Yun, Y.P., Lee, J.H., Wagner, H. and Shin, YS. (1985). Inhibition of lens-aldose reductase activity by brazilin and haematoxylin. Planta Med. 47, 66-67 
    21. Olefsky, J.M. (1975). Effect of dexamethasone on insulin binding, glucose transport, and glucose oxidation of isolated rat adipocytes. J. Clin. Invest. 56, 1499-1508 
    22. Sommer, D., Coleman, S., Swanson, S.A. and Stemmer, P.M. (2002). Differential susceptibilities of serine/threonine phosphatases to oxidative and nitrosative stress. Arch. Biochem. Biophys. 404,271-278 
    23. Tanti, J.F., Grillo, S., Gremeaux, T., Coffer, P.J., Van Obberghen, E. and Le Marchand-Brustel, Y. (1997). Potential role of protein kinase B in glucose transporter 4 translocation in adipocytes. Endocrinology. 138, 2005-2010 
    24. Tonks, N.K., Diltz, CD. and Fischer, E.H. (1988). Characterization of the major protein-tyrosine-phosphatases of human placenta. J. Biol. Chem. 263,6731-6737 
    25. Ugi, S., Imamura, T., Maegawa, H., Egawa, K., Yoshizaki, T., Shi, K., Obata, T., Ebina, Y., Kashiwagi, A. and Olefsky, J.M. (2004). Protein phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (protein kinase B) activity in 3T3-Ll adipocytes. Mol. Cell Biol. 24, 8778-8789 
    26. Yang, K.M., Jeon, S.D., So, D.S. and Moon, C.K. (2000). Brazilin augments cellular immunity in multiple low dose streptozotocin (MLD-STZ) induced type I diabetic mice. Arch. Pharm. Res. 23, 626-632 

 저자의 다른 논문

  • Khil, Lee-Yong (4)

    1. 1996 "Effects of Hydroxybrazilin on Glutathione Depletion Induced by $\textrm{BrCCl}_3$ and Menadione in Cultured Rat Hepatocytes" 환경독성학회지 = Journal of environmental toxicology 11 (3): 53~57    
    2. 1996 "사염화탄소의 간독성에 대한 파두 다당류분획의 예방효과" 환경독성학회지 = Journal of environmental toxicology 11 (3): 59~63    
    3. 1996 "AIIoxan 유도 당뇨병상태에서 과산화지질생성에 미치는 Brazilin의 효과" 환경독성학회지 = Journal of environmental toxicology 11 (3): 69~73    
    4. 1998 "Brazilin Inhibits Activities of Protein Kinase C and Insulin Receptor Serine Kinase in Rat Liver" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 21 (2): 140~146    
  • Moon, Chang-Kiu (73)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기