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The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology v.12 no.4, 2004년, pp.250 - 256  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Characterization of Acetoxyscirpendiol of Paecilomyces tenuipes as Inhibitor of Sodium Glucose Co-transporters Expressed in Xenopus laevis Oocytes

Park, Il-Woon    (Department of Life Sciences, University of Seoul   ); Hwang, Gwi-Seo    (College of Oriental Medicine Kyungwon University   ); Kim, Ha-Won    (Department of Life Sciences, University of Seoul   ); Lee, Dong-Hee    (Department of Life Sciences, University of Seoul  );
  • 초록

    Cordyceps possesses numerous health-promoting ingredients including hypoglycemic agents. The mechanism for the reduction of circulatory sugar content, however, is still not fully understand. In this study, 4-beta acetoxyscirpendiol (ASD) was purified from the methanolic extracts from fruiting bodies of Paecilomyces tenuipes. Na+/Glucose transporter-1 (SGLT-1) was expressed in the Xenopus oocytes. The effect of ASD on the oocyte expressed SGLT-1 was analyzed utilizing the voltage clamp and 2-deoxy-D-glucose (2-DOG) uptake studies. ASD was shown to significantly inhibit SGLT-1 activity compared to the non-treated control in a dose- dependent manner. In the presense of its two derivatives (diacetoxyscirpenol or 15-acetoxyscirpendiol), SGLT-1 activity was greatly inhibited similarly as ASD. Between ASD derivatives, 15-acetoxyscirepenol showed inhibition equivalent to that of ASD while diacetoxyscirpenol did less degree of inhibition. Insummary , these results strongly indicate that ASD in P. tenuipes may serve as a functional substance in lowering blood sugar in the circulatory system. ASD and its derivatives can be utilized as inhibitors of SGLT-1.


  • 주제어

    SGLT-1 .   Acetoxyscirpendiol .   Cordyceps .   Diabetes .   Voltage clamp .   Xenopus oocytes.  

  • 참고문헌 (18)

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