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大韓獸醫學會誌 = Korean journal of veterinary research v.44 no.4 = no.117, 2004년, pp.561 - 569   피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

이유자돈 전신소모성증후군 이환 자돈에서의 바이러스성 원인체 검색 및 porcine circovirus 2 분리동정
Detection of viral pathogens and isolation of porcine circovirus 2 from postweaning multisystemic wasting syndrome-affected piglets

박최규    (국립수의과학검역원   ); 김현수    (충남대학교 수의과대학  );
  • 초록

    To detect viral agents and isolate porcine circovirus 2 (PCV2), 60 samples of lung and lymph node were collected from 5 to 12 week-old pigs that had showed clinical signs of postweaning multisystemic wasting syndrome (PMWS). Polymerase chain reactions (PCRs) were conducted to identify the viral pathogens including PCV1, PCV2, porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) that have been considered to be the causal agents of PMWS. Among 60 samples, PCV 2 was detected from 57 samples but no PCV 1 was detected. PRRSV and/or PPV were also detected from 27 (47.4%) samples and 1 (1.8%) sample of these 57 PCV 2-positive samples, respectively. Tissue homogenates were inoculated onto PCV-free PK-15 cell monolayers. Seven isolates were confirmed as PCV 2 by multiplex PCR, indirect immunofluorescence assay, and transmissible electron microscopy. These date suggest that PRRSV is a major cofactors causing PMWS in pigs that were infected with PCV2 in Korea.


  • 주제어

    postweaning multisystemic wasting syndrome(PMWS) .   porcine circovirus(PCV) .   porcine parvovirus( PPV) .   porcine reproductive and respiratory syndrome virus(PRRSV) .   polymerase chain reaction(PCR).  

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  • 이 논문을 인용한 문헌 (1)

    1. Kim, Eun-Gyeong ; Hwang, Bo-Won ; Lee, Jong-Min ; Son, Byeong-Guk ; Park, Ho-Jung ; Kim, Tho-Kyoung 2009. "Rapid detection and quantification of porcine circovirus type 2 (PCV 2) DNA in Real-time PCR" 韓國家畜衛生學會誌 = Korean journal of veterinary service, 32(4): 299~306     

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