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Mass Production of HzSNPV Baculoviruses in Immobilized Heliothis zea (HzAM1) Insect Cell Culture

Son Jeong Hwa    (Department of Biotechnology & Bioengineering, Pukyong National University   ); Buchholz Rainer    (Department of Bioprocess Engineering, Friedrich-Alexander University   ); Kim Sung-Koo    (Department of Biotechnology & Bioengineering, Pukyong National University  );
  • 초록

    Heliothis zea (HzAM1) insect cells were immobilized in microspheres by sodium-cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). The highest HzAMl cell density was $7.5{\times}10^7$ cells/mL in the microspheres. After infection of the immobilized cells by Heliothis zea single nuclear polyhedrosis virus (HzSNPV), the highest concentration of HzSNPV (polyhedral inclusion bodies: PIBs) produced was $2.87{\times}10^{10}$ PIBs/mL in the microspheres.


  • 주제어

    HzAM1 insect cell .   HzSNPV .   NaCS .   PDADMAC .   PIBs.  

  • 참고문헌 (14)

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