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Biotechnology and bioprocess engineering v.9 no.5, 2004년, pp.379 - 382   피인용횟수: 1

Partial Purification and Characterization of an Extracellular Protease from Xenorhabdus nematophilus a Symbiotic Bacterium Isolated from an Entomopathogenic Nematode, Steinernema glaseri

Chae Young-Rae    (School of Chemical Engineering and Bioengineering, University of Ulsan   ); Ryu Keun-Garp    (School of Chemical Engineering and Bioengineering, University of Ulsan  );
  • 초록

    Entomopathogenic nematodes are used for insect control. Herein, an extracellular protease was partially purified from a culture supernatant of Xenorhabdus nematophilus, a symbiotic bacterium of an entomopathogenic nematode, Steinernema glaseri: using precipitation with $80\%$ v/v isopropyl alcohol followed by gel permeation chromatography with a packed Sephacryl S-300 HR media. The partially purified protease exhibited maximal activity at pH 7 in the presence of 1 mM $CaCl_2$ . The protease was identified as a metallo-protease based on the inhibition of its activity by the metal chelating agent, EDTA.


  • 주제어

    entomopathogenic nematodes .   inhibitor .   protease .   symbiotic bacteria.  

  • 참고문헌 (10)

    1. Ryu, K, J. S. Bae, and S. H. Park (1999) Extracellular protease production from Xenorhabdus nematophilus, a symbiotic bacterium of entomopathogenic nematodes. Biotechnol. Bioprocess Eng. 4: 147-150 
    2. Akhurst, R. J. (1980) Morphological and functional dimorhpism in Xenorhabdus spp., bacteria symbiologically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis. J. Gen. Microbial. 121: 303-309 
    3. Smigielski, A. J., R. J. Akhurst, and N. E. Boemare (1994) Phase variation in Xenorhabdus nematophilus and Photorhabdus luminescens: differences in respiratory activity and membrane energization. Appl. Environ. Microbiol. 60: 120-125 
    4. Park, S. H. and D. W. Kim (1997) Environmentally friendly biopesticide using entomopathogenic nematodes. Kor. J. Biotechnol. Bioeng. 12: 261-268 
    5. Wang, H. and B. C. A. Dowds (1993) Phase variation in Xenorhabdus luminescens: Cloning and sequencing of the lipase gene and analysis of its expression in primary and secondary phases of the bacterium. J. Bacteriol. 175:1665-1673 
    6. Georgis, R. (1992) Present and future prospects for entomopathogenic nematode products. Biocontrol Sci. Technol. 2: 83-99 
    7. Caldas, C., A. Cherqui, A. Pereira, and N. Simoes (2002) Purification and characterization of an extracellular protease from Xenorhabdus nematophila involved in insect immunosuppression. Appl. Environ. Microbiol. 68: 1297-1304 
    8. Schmidt, T. M., B. Bleakley, and K. H. Nealson (1988) Characterization of an extracellular protease from the insect pathogen Xenorhabdus luminescens. Appl. Environ. Microbiol. 54: 2793-2797 
    9. Park, S. H. and Y. S. Yu (1999) Isolation and culture characteristics of a bacterial symbiont from entomopathogenic nematode Steinernema glaseri. Kor. J. Biotechnol. Bioeng. 14: 198-204 
    10. Bowen, D., T. A. Rocheleau, M. Blackburn, O. Andreev, E. Golubeva, R. Bhartia, and R. H. ffrench-Constant (1998) Insecticidal toxins from the bacterium Photorhabdus luminescens. Science 280: 2129-2132 
  • 이 논문을 인용한 문헌 (1)

    1. Yun, Won-Kap ; Bae, Hyun-Ji ; Kim, You-Jeong ; Kwon, O Jun ; Im, Moo Hyeog ; Cho, Hyun Duk ; Kim, Taewan 2014. "Effect of the supplementation of Coconopsis lanceolata extract on lipid metabolism amelioration in type 2 diabetes mouse model induced by high fat diet" 한국식품저장유통학회지 = Korean journal of food preservation, 21(1): 107~113     

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