본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Biotechnology and bioprocess engineering v.9 no.5, 2004년, pp.383 - 388   피인용횟수: 3

Conversion of G. hansenii PJK into Non-cellulose-producing Mutants According to the Culture Condition

Park, Joong-Kon    (Department of Chemical Engineering, Kyungpook National University   ); Hyun, Seung-Hun    (Department of Chemical Engineering, Kyungpook National University   ); Jung, Jae-Yong    (Department of Chemical Engineering, Kyungpook National University  );
  • 초록

    The conversion of a cellulose-producing cell ( $Cel^+$ ) from Gluconacetobacter hansenii PJK (KCTC 10505 BP) to a non-cellulose-producing cell ( $Cel^-$ ) was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles, which exerted a strong shear stress. The addition of organic acid, such as glutamic acid and acetic acid, induced the conversion of microbial cells from a wild type to $Cel^-$ mutants in a flask culture. The supplementation of $1\%$ ethanol to the medium containing an organic acid depressed the con-version of the microbial cells to $Cel^-$ mutants in a conventional flask without slanted baffles. The addition of ethanol to the medium containing an organic acid; however, accelerated the conversion of microbial cells in the flask with slanted baffles. The $Cel^+$ cells from the agitated culture were not easily converted into $Cel^-$ mutants on the additions of organic acid and ethanol to a flask without Slanted baffles, but some portion of the $Cel^+$ cells were converted to $Cel^-$ mutants in a flask with slanted baffles. The conversion ratio of $Cel^+$ cells to $Cel^-$ mutants was strongly re-lated to the production of bacterial cellulose independently from the cell growth.


  • 주제어

    bacterial cellulose .   cellulose-negative mutant .   Gluronacetobacter hansenii .   ethanol .   organic acid .   Shear stress.  

  • 참고문헌 (19)

    1. Orodera, M., I. Harashima, K. Toda, and T. Asakura (2002) Silicone rubber membrane bioreactors for bacterial cellulose production. Biotechnol. Bioprocess Eng. 7: 289-294     
    2. Schramm, M. and S. Hestrin (1954) Factors affecting production of cellulose at the air/liquid interface of a culture of Acetobacter xylinum. J. General Microb. 11: 123-129 
    3. Lee, H. C. (1999) Medium for producing microbial cellulose and preparation method of microbial cellulose using the same. Korea Patent KB10-0197357 
    4. Vandamme, E. J., S. De Baets, A. Vanbaelen, K. Joris, and P. De Wulf (1998) Improved production of bacterial cellulose and its application potential. Polym. Degrad. Stabil. 59: 93-99 
    5. Pyun, Y. R. (2002) Method of manufacturing microbial cellulose employing soybean processed product. Korea Patent KP2002-0080802 
    6. Delmer, D. P. and Y. Arnor (1995) Cellulose biosynthesis. Plant Cell 7: 987-1000 
    7. Park, J. K., Y. H. Park, and J. Y. Jung (2003) Production of bacterial cellulose by Gluconacetobacter hansenii isolated from rotten apple. Biotechnol. Bioprocess Eng. 8: 83-88     
    8. Son, H. J., O. M. Lee, Y. G. Kim, Y. K. Park, and S. J. Lee (2000) Characteristics of cellulose production by Acetobacter sp. A9 in static culture. Kor. J. Biotechnol. Bioeng. 15: 573-577 
    9. Toyosaki, H., T. Naritomi, A. Seto, M. Matsuoka, T. Tsuchida, and F. Yoshinaga (1995) Screening of bacterial cellulose-producing Acetobacter strains suitable for agitated culture. Biosci. Biotechnol. Biochem. 59: 1498-1502 
    10. Yoshino, T., T. Asakura, and K. Toda (1996) Cellulose production by Acetobacter pasteurianus on silicone membrane. J. Ferment. Bioeng. 81: 32-36 
    11. Klemm D., D. Schumann, U. Udhard, and S. Marsch (2001) Bacterial synthesized cellulose: Artificial blood vessels for microsurgery. Prog. Polym. Sci. 26: 1561-1603 
    12. Park, J. K., J. Y. Jung, and Y. H. Park (2003) Cellulose production by Gluconacetobacter hansenii in a medium containing ethanol. Biotechnol. Lett. 25: 2055-2059 
    13. Brown, A. J. (1886) An acetic acid ferment which forms cellulose. J. Chem. Soc. 49: 432-439 
    14. Coucheron, D. H. (1991) An Acetobacter xylinum inser· tion sequence element associated with inactivation of cellulose production. J. Bacterial. 173: 5723-5731 
    15. Jeong, Y. J. and I. S. Lee (2000) A view of utilizing cellulose produced by Acetobacter bacteria. Food Ind. Nutr. 5: 25-29 
    16. Cannon, R. E. and S. M. Anderson (1991) Biogenesis of bacterial cellulose. Crit. Rev. Microbial. 17: 435-447 
    17. Valla, S. and J. Kjosbakken (1981) Cellulose-negative mutants of Acetobacter xylinum. J. General Microb. 128: 1401-1408 
    18. Matthysse, A. G., D. L. Thomas, and A. R. White (1995) Mechanism of cellulose synthesis in Agrobacterium tume· faciens. J. Bacterial. 177: 1076- 1081 
    19. Yamanaka, S., K. Watanabe, N. Kitamura, M. Iguchi, S. Mitsuhashi, Y. Nishi, and M. Uryu (1989) The structure and mechanical properties of sheets prepared from bacterial cellulose. J. Mat. Sci. 24: 3141-3145 
  • 이 논문을 인용한 문헌 (3)

    1. 2008. "" Biotechnology and bioprocess engineering, 13(2): 240~247     
    2. Khan, Salman ; Shehzad, Omer ; Khan, Taous ; Ha, Jung Hwan ; Park, Joong Kon 2009. "Production of Bacterial Cellulose by Gluconacetobacter hansenii Using a New Bioreactor Equipped with Centrifugal Impellers" 화학공학 = Korean chemical engineering research, 47(4): 506~511     
    3. 2010. "" Biotechnology and bioprocess engineering, 15(1): 110~118     

 저자의 다른 논문

  • 박중곤 (26)

    1. 1996 "전세포 Escherichia coli 의 캡슐고정화" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 11 (4): 398~404    
    2. 1997 "The Recovery of Heavy Metals Using Encapsulated Microbial Cells" Biotechnology and bioprocess engineering 2 (2): 132~135    
    3. 1999 "Pseudomonas putida에서 생산된 전세포 benzoylformate decarboxylase의 활성특성 및 고정화 캡슐 제조" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 14 (3): 264~272    
    4. 1999 "산소공급이 캡슐고정화 Aspergillus niger의 구연산 생산에 미치는 영향" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 14 (6): 672~676    
    5. 2000 "Immobilization of Cyclodextrin Glucanotrasferase on Amberline IRA-900 for Biosynthesis of Transglycosylated Xylitol" Biotechnology and bioprocess engineering 5 (3): 174~180    
    6. 2000 "캡슐 고정화 전세포 CGTase를 이용한 Glucosyl-xylitol 생산" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 15 (1): 35~41    
    7. 2001 "선택적 분리를 위한 분자 각인 고분자의 설계 및 응용" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 16 (2): 115~122    
    8. 2001 "Encapsulation of Whole Cell CGTase from Concentrated Broth Solution" Biotechnology and bioprocess engineering 6 (1): 67~71    
    9. 2002 "습식 상 전이법으로 제조된 Theophylline 각인 대칭 막의 선택적 흡착" 한국생물공학회지 = Korean journal of biotechnology and bioengineering 17 (2): 207~211    
    10. 2002 "High frequency Plant Regeneration of Garlic (Allium sativum L.) Calli Immobilized in Calcium Alginate Gel" Biotechnology and bioprocess engineering 7 (4): 206~211    
  • Hyun, Seung-Hun (0)

  • 정재용 (5)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기