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Biotechnology and bioprocess engineering v.9 no.5, 2004년, pp.405 - 413   피인용횟수: 1

Physicochemical and Rheological Properties of a Novel Emulsifier, EPS-R, Produced by the Marine Bacterium Hahella chejuensis

Yim Joung Han    (Korea Polar Research Institute, KORDI   ); Kim Sung Jin    (Korea Polar Research Institute, KORDI   ); Aan Se Hoon    (Korea Polar Research Institute, KORDI   ); Lee Hong Kum    (Korea Polar Research Institute, KORDI  );
  • 초록

    The rheological properties of an exopolysaccharide, EPS-R, produced by the marine bacterium Hahella chejuensis strain 96CJ 10356 were investigated. The $E_{24}$ of $0.5\%$ EPS-R was $89.2\%$ , which was higher than that observed in commercial polysaccharides such as xanthan gum ( $67.8\%$ ), gellan gum ( $2.01\%$ ) or sodium alginate ( $1.02\%$ ). Glucose and galactose are the main Sugars in EPS-R, with a molar ratio of ${\~}1:6.8$ , xylose and ribose are minor sugar components. The average molecular mass, as determined by gel filtration chromatography, was $2.2{\times}10^3$ KDa, The intrinsic viscosities of EPS-R were calculated to be 16.5 and 15.9 dL/g using the Huggins and Kraemer equations, respectively, with a 2.3 dL/g overlap. In terms of rigidity, the conformation of EPS-R was similar to that of caboxymethyl cellulose ( $5.0{\times}10^{-2}$ ). The rheological behavior of EPS-R dispersion indicated that the formation of a structure intermediate between that of a random-coil polysaccharide and a weak gel. The aqueous dispersion of EPS-R at concentrations ranging from 0.25 to $1.0\%$ (w/w) showed a marked shear-thinning property in accordance with Power-law behavior. In aqueous dispersions of $1.0\%$ EPS-R, the consistency index (K) and flow behavior index (n) were 1,410 and 0.73, respectively. EPS-R was Stable to pH and salts.


  • 주제어

    exopolysacchalide .   Hahella chejuensis .   marine bacterium .   rheological properties.  

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  • 이 논문을 인용한 문헌 (1)

    1. Ryu, Jeong-Eun ; Lee, Young-Nam 2009. "Optimal Culture Conditions for MK1 Strain Isolated from Soft-Rotten Tissue of Neungee Mushroom (Sarcodon aspratus) and the Physico-Chemical Properties of the Purified Exopolysaccharide of MK1" Korean journal of microbiology = 미생물학회지, 45(4): 324~331     

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