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Kinetic Property and Phylogenie Relationship of 2-Hydroxy-muconic Semialdehyde Dehydrogenase Encoded in tomC Gene of Burkholderia cepacia G4

Reddy, Alavala-Matta   (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National UniversityUU0001309  ); Min, Kyung-Rak   (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National Universit  ); Lee, Kyoung   (College of Natural Science, Changwon National Universit  ); Lim, Jai-Yun   (College of Natural Sciences, Chungbuk National Universit  ); Kim, Chi-Kyung   (College of Natural Sciences, Chungbuk National Universit  ); Kim, Young-Soo   (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National UniversityUU0001309  );
  • 초록

    2-Hydroxymuconic semialdehyde (2-HMS) dehydrogenase catalyzes the conversion of 2-HMS to 4-oxalocrotonate, which is a step in the meta cleavage pathway of aromatic hydrocarbons in bacteria. A tomC gene that encodes 2-HMS dehydrogenase of Burkholderia cepacia G4, a soil bacterium that can grow on toluene, cresol, phenol, or benzene, was overexpressed into E. coli HB 101, and its gene product was characterized in this study. 2-HMS dehydrogenase from B. cepacia G4 has a high catalytic efficiency in terms of V $_{max}$ K $_{max}$ towards 2-hydroxy-5-methyl-muconic semialdehyde followed by 2-HMS but has a very low efficiency for 5-chloro-2-hydroxymuconic semialdehyde. However, the enzyme did not utilize 2-hydroxy-6-oxo-hepta 2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid as substrates. The molecular weight of 2-HMS dehydrogenase from B. cepacia G4 was predicted to be 52 kDa containing 485 amino acid residues from the nucleotide sequence of the tomC gene, and it exhibited the highest identity of 78% with the amino acid sequence of 2-HMS dehydrogenase that is encoded in the aphC gene of Comamonas testosteroni TA441. 2-HMS dehydrogenase from B. cepacia G4 showed a significant phylogenetic relationship not only with other 2-HMS dehydrogenases, but also with different dehydrogenases from evolutionarily distant organisms.sms.


  • 주제어

    Burkholderia cepacia G4 .   2-Hydroxymuconic semialdehyde dehydrogenase .   Kinetic paramenter .   Phylogenetic relationship.  

  • 참고문헌 (16)

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  • Kim , Young-Soo (52)

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    2. 1990 "흰쥐의 다리종양에서 발현된 케이라스 암유전자" 한국생화학회지 23 (2): 185~189    
    3. 1991 "Pseudomonas putida의 Catechol 2,3-dioxygenase 유전자의 클로닝" Korean journal of microbiology = 미생물학회지 29 (3): 155~159    
    4. 1992 "Molecular Cloning and M13 Subcloning of Genes Encoding Catechol Dioxygenases" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 15 (1): 48~51    
    5. 1992 "Cloning and Overexpression of Methylcatechol 2, 3-Dioxygenase Gene from Toluene-Degrading Pseudomonas putida mt-2(pWWO)" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 15 (4): 360~364    
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