본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

광합성 섬모류 Mesodinium rubrum MR-MAL01의 응용성(1) 이매패류 종묘생산을 위한 먹이생물 유용성
Applicability of a Photosynthetic Ciliate, Mesodinium rubrum MR-MAL01 -Usefulness as a Live Prey Species for the Marine Aquaculture of Bivalves-

김형섭   (군산지방해양수산청CC0187504  ); 명금옥   (군산대학교 해양정보과학UU0000277  ); 조수근   (군산대학교 해양생명과학UU0000277  ); 이원호   (군산대학교 해양정보학과UU0000277  );
  • 초록

    최근, 곰소만에서 채집한 시료로부터 최초의 온대역산 광합성 섬모류 Mesodinium rubrum MR-MAL01 배양체를 분리ㆍ배양하였다. 이 섬모류를 이용하여 이매패류의 섭식실험을 수행한 결과, 비단가리비와 바지락과 같은 이매패류가 섬모류를 직접 섭식 하였음을 확인하였는데, 개체 당 최대 섭식률은 비단가리비 9,590 cells $min^{-1}$ , 바지락 23,200 cells $min^{-1}$ 으로 나타났다. 또한 비단가리비의 소화관에서 완전한 M. rubrum세포를 확인하지 못했으나, 파괴된 세포로 생각되는 작은 입자들을 관찰하였다. 바지락 치패에 섬모류인 M. rubrum과 미소조류인 Isochrysis galbana (KMCC H-2)를 공급하면서 28일 동안 사육한 결과, 작은 치패 그룹(각장 0.46 mm)에서는 I. galbana를 공급한 실험구가 좋은 성장을 보였던 반면, 큰 치패 그룹(각장 0.84 mm)에서는 M. rubrum을 공급한 실험구가 I. galbana를 공급한 실험구에 비해 성장이 좋았다. 따라서 이 광합성 섬모류는 이매패류의 치패 및 모패와 입이 작은 자어의 먹이생물, rotifer의 영양강화제 및 어류의 천연 착색제로도 활용할 수 있는 잠재력이 있어 해양 생물공학적 활용을 위한 생물재료로서 개발 가능성을 제안한다.

    In a series of feeding experiments using the newly established the first laboratory strain of a temperate photosynthetic ciliate, Mesodinium rubrum MR-MAL01, direct evidence was obtained for ingestion of the cultured M. rubrum cells by the bivalves like the Korean scallop, Chlamys farreri and Manila clam. Ruditapes phil-ippinarum. Each experimental Korean scallop and Manila clam removed up to 9,590 and 23,200 cells min-1, respectively. Small particles almost identical to the ruptured cells of MR-MAL01 culture were observed in the gut of the experimental bivalves, although no intact M. rubrum cell was found. In a 28 days rearing experiment, Isoshrysis galbana (KMCC H-2), a microalga, supported better growth of small Manila clam spat (0.46 mm in shell length) than MR-MAL01 strain. For the large Manila clam spat (0.84 mm in shell length), however. MR-MAL01 strain was a better prey item. Mass culture methods for this photosynthetic marine ciliate may be developed for use as live feed in aquaculture of the spat and broodstock of bivalve and small-mouthed fish larvae.

  • 주제어

    Photosynthetic ciliate .   Live prey .   Korean scallop .   Manila clam.  

  • 참고문헌 (40)

    1. Red-coloured digestive glands in cultured mussels and scallops:the implication of Mesodinium rubrum , Carver,C.E.;A.L.Mallet;R.Warnock;D.Douglas , J. Shellfish Research / v.15,pp.191-201,
    2. The distribution of ichthyoplankton in Southampton water in response to low oxygen levels produced by a Mesodinium rugrum bloom , Hayes,G.C.;D.A.Purdie;J.A.Williams , J. Fish Biology / v.34,pp.811-813,
    3. Absorbance spectrum for Mesodinium rubrum MR-MAL01, a marine photosynthetic ciliate, fed on photo-adapted cryptophyte , Kim,H.S.;G.Myung;S.K.Jo;W.Yih , "The Sea" J. of Kor. Soc. Oceanogr. / v.8,pp.29-34,
    4. Ecological factors controlling the interspecific interation between laboratory strains of Mesodinium rubrum, a marine photosynthetic ciliate, and its cryptophyte prey , Myung,G. , Ph. M. thesis, Kunsan Nat'l. Univ. / v.,pp.59,
    5. NFRDI , Recent red tides in Korean coastal waters / v.,pp.280,
    6. Nutritional effects of algal addition in first-feeding of turbot (Scophthalmus maximus L.)larvae , Reitan K.I.;J.R.Rainuzzo;G.Øie;Y.Olsen , Aquaculture / v.118,pp.257-275,
    7. Changes in bacterial populations during red tides caused by Mesodinium rubrum and Gymnodinium catenatum in North West Coast of Spain , Romalde,J.L.;A.E.Toranzo;J.L.Barja , J. Applied Bacteriology / v.68,pp.123-132,
    8. The role of phytoplankton in the dissolved oxygen budget of a stratified estuary , Soulsby,P.G.;M.Mollowney;G.Marsh;D.Lowthion , Water Science Technology / v.17,pp.145-156,
    9. Optimum culture conditions of four species of microalgae as live food from China , Park,J.E.;S.B.Hur , J. of Aquaculture / v.13,pp.107-117,
    10. A review of the nutritional effects of algae in marine fish larvae , Reitan K.I.;J.R.Rainuzzo;G.Øie;Y.Olsen , Aquaculture / v.155,pp.207-221,
    11. The planktonic phototrophic ciliate, Mesodinium rubrum, as a useful organism for marine biotechnological applications , Yih W.;J.H.Shim , J. Mar. Biotechnol. / v.5,pp.82-85,
    12. Some observations on vertical distribution and migration of the phototrophic ciliate Mesodinium rubrum (Myrionecta rubra) in a stratified brackish inlet , Crawford,D.W.;T.Lindholm , Aquatic Microb. Ecol. / v.13,pp.267-274,
    13. "Red" oysters (Ostrea deulis L.) caused by Mesodinium rubrum in Lake Grevelingen , Kat,M. , Aquaculture / v.38,pp.375-377,
    14. Photosynthetic in Mesodinium rubrum: species-specific measurements and comparison to community rates , Stoecker,D.K.;M.Putt;L.H.Dabis;A.E.Michaels , Mar. Eco. Prog. Ser. / v.73,pp.245-252,
    15. Changes in the sea-ice brine community during the spring-summer transition, McMurdo sound, Antarctica. 1. Photosynthetic protists , Stoecker,D.K.;K.R.Buck;M.Putt , Mar. Ecol. Prog. Ser. / v.84,pp.265-278,
    16. Reported red-water outbreaks and their effects on fauna of the west and south coasts of South Africa, 1959-1980 , Horstman,D.A. , Fisheries Bulletin, Sea Fisheries Institute, Republic of South Africa / v.15,pp.71-88,
    17. Intensification of color of fancy red carp with zeaxanthin and myxoxanthophyll, major cartenoid constituents of Spirulina , Matsuno,T.;S.Nagata;M.Iwahashi;T.Koike;M.Okada , Nippon Suisan Gakkaishi / v.45,pp.627-532,
    18. Variation of ascorbic acid content in diffenent live food organisms , Merchie,G.;P.Lavens;Ph.Dhert;M.Dehasque;H.Nelis;A.De Leenheer;P.Sorgeloos , Aquaculture / v.134,pp.325-337,
    19. Cartenoids in diets for salmonids. Ⅰ. Pigmentation of rainbow trout with the indivisual isomers of astaxanthin in comparison with conthaxanthin , Foss,P.;T.Storebakken;K.Schiedt;S.Liaaen-Jensesn;E.Austreng;K.Streiff , Aquaculture / v.41,pp.213-226,
    20. Cryptophyte algae are robbed of their organelles by the marine ciliate Mesodinium rubrum , Gustafson,D.E.;D.K.Stoecker;M.D.Johnson;W.F.Van Heukelem;K.Sneider , Nature / v.405,pp.1049-1052,
    21. Abundance, chlorophyll content and photosynthetic rates of ciliates in the Nordic Sea during summer , Putt,M. , Deep Sea Res. / v.37,pp.1713-1731,
    22. Studies on spat production of the Sun and Moon scallop, Amusium japonicum japonicum (GMELIN) , Son,P.W.;D.S.Ha;S.Rho;D.S.Chang;C.H.Lee;D.K.Kim , J. of Aquaculture / v.11,pp.371-380,
    23. Red "water-bloom" in british columbia waters , Clemens,W.A. , Nature / v.152,pp.473,
    24. Population dynamics and environmental conditions for the continuous growth of the phototrophic ciliate, Mesodinium rubrum in Korean West Coast , Kim,H.S. , Ph.D. thesis,Kunsan Nat'l. Univ. / v.,pp.159,
    25. Mesodinium rubrun-a unique photosynthetic ciliate , Lindholm,T. , Advances in Aquatic Microbiology / v.3,pp.1-48,
    26. The red-water ciliate Mesodinium rubrum and its "incomplete symbionts": a review including new ultrastructural observations , Taylor,F.J.R.;D.J.Blackbourn;J.Blackbourn , J. Fish. Res. Board. Can. / v.28,pp.391-407,
    27. Artificial mass culture of Flat oyster larvae, Ostrea denselamellosa, and collection rates according to various spat collection methods , Yang,M.H.;H.S.Kim;J.Y.Lee;C.H.Han , Kor. J. Malacol. / v.17,pp.35-44,
    28. Studies on the collection and growth of spat Surf clam, Spisula sachalinensis in the tank , Lee,J.Y.;W.K.Kim;C.S.Lee;Y.J.Park , J. of Aquaculture / v.15,pp.111-117,
    29. Depth maxima of Mesodinium rubrum examples from a stratified Baltic Sea inlet , Lindholm,T.;A.C.Mork , Sarsia / v.75,pp.53-64,
    30. A red water-bloom caused by the autotrophic ciliate, Mesodinium rubrum, in the austral summer in the fast ice area near Syowa Station, Antarctica with note on their photosynthetic rate , Satoh,H.;K.Watanabe , J. Tokyo Univ. Fish. / v.78,pp.11-17,
    31. Ingestion of cryptophyte cells by the marine photosynthetic ciliate Mesodinium rubrum , Yih W.;H.S.Kim;H.J.Jeong;G.Myung;Y.G.Kim , Aquatic Microb. Ecol. / v.,pp.,
    32. Observations on blooms of Mesodinium rubrum in the upwelling area off Ecuador. Oceanologica Acta, Supplementum 1987 , Jimenez,R.;P.Intriago , Proceedings of International Symposium on Equatorial Vertical Motion, Paris, 6-10. May 1985 / v.,pp.145-154,
    33. Mesodinium rubrum - a photosynthetic ciliate , Lindholm,T.;W.Reisser(ed.) , Algae and symbioses: Plants, Animals, Fungi, Virus, Interactions Explored / v.,pp.501-514,
    34. The annual cycle of Mesodinium rubrum in the waters surrounding the Isles of Shoals, Gulf of Maine , Montagnes,D.J.S.;D.H.Lynn , J. Plankto Research / v.11,pp.193-201,
    35. Red tide organisms in the coastal waters of Cheju island, Southern Korea , Yoon,Y.H.;H.K.Rho;Y.G.Kim , Bull. Mar. Res. Inst. / v.15,pp.1-14,
    36. Mesodinium rubrum in the Baja California upwelling system , Packard,T.T.;D.Blasco;R.T.Barber;Boje R.;M.Tomczak(eds.) , Upwelling Ecosystems / v.,pp.73-89,
    37. Blooms of Mesodinium rubrum and resulting salmon mortalities and stress during 1998 and 1999 in the Bay of Fundy, Eastern Canada , Martin,J.L.;F.Page;pM.Dowd;M.Ringuette;M.M.LeGresley , Harmful Algal Blooms 2000, Proceedings of 9th International Conference on Algal Blooms, Tasmania, Australia, 6-11 Feb / v.,pp.173,
    38. Mesodinium rubrum: The phytoplankter that wasn't , Crawford,D.W. , Mar. Ecol. Prog. Ser. / v.58,pp.161-174,
    39. A study on seedling production of Jicon scallop, Chlamys farreri. 1. Spawning, development and rearing of larvae , Na,G.H.;W.G.Jeong;C.H.Cho , J. of Aquaculture / v.8,pp.307-316,
    40. Enhanced first feeding of halibut larvae (Hippoglossus hippoglossus L.) in green water , Nass,K.E.;T.Naess;T.Harboe , Aquaculture / v.105,pp.143-157,

 저자의 다른 논문

  • 명금옥 (3)

    1. 2003 "광적응된 은편모조류를 섭식한 해양 광합성 섬모류 Mesodinium rubrum MR-MAL01의 흡광스펙트럼" 바다 : 한국해양학회지 8 (1): 29~34    
    2. 2005 "금강하구언 담수방류와 춘계 식물플랑크톤 군집의 단주기 변동" 바다 : 한국해양학회지 10 (3): 154~163    
    3. 2005 "춘계 금강 하구에서 혼합영양 섬모류인 Myrionecta rubra (=Mesodinium rubrum) 개체군의 단주기 변동" Algae 20 (3): 207~216    
  • Jo, Soo-Gun (13)

  • 이원호 (27)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • NDSL :

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기