본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

간암세포 (HepG2 Cell)에서의 식이성 CLA(Conjugated Linoleic Acid)가 항동맥경화성 인자에 미치는 영향
Effect of CLA (Conjugated Linoleic Acid) on the Anti-Atherosclerotic actors in Human Hepatoma HepG2 Cells

오현희   (성신여자대학교 식품영양학과UU0000771  ); 문희정   (성신여자대학교 식품영양학UU0000771  ); 이명숙   (성신여자대학교 식품영양학과UU0000771  );
  • 초록

    Conjugated linoleic acid (CLA) is the mixture of positional and geometric isomers of linoleic acid (LA), which is found abundantly in dairy products and meats. This study was performed to investigate the anticarcinogenic effect of CLA in HepG2 hepatoma cells. HepG2 cell were treated with LA and CLA at the various concentrations of 10, 20, 40, 80 uM each at different incubation times. After each incubation times, cell proliferation, fatty acids incorporation into cell, peroxidation and postaglandin E $_2$ (PGE $_2$ ) and thromboxane $A_2$ (TXA $_2$ ) for the eicosanoid metabolism were measured. LA treated HepG2 cells were increased cell growth 6 - 70% of control whereas CLA increased cell death the half of those in LA group (p 〈 0.001). LA and CLA were incorporated very well into the cellular membranes four times higher than in control according to concentration and longer incubation times. Moreover, LA synthesized significantly arachidonic acids corresponding with LA concentration compared to CLA supplementation. The supplementation with LA increased intracellular lipid peroxides concentration corresponding with LA concentration and five times higher than those in CLA significantly at any incubation times (p 〈 0.001). PGE $_2$ and TXA $_2$ levels were three to twenty times lower in condition of CLA treatments than LA, respectively. Overall, the dietary CLA might change the HepG2 cell growth by the changes of cell composition, production of lipid peroxide. Since CLA have not changed the levels of arachidonic acid of cell membrane, which was sources of eicosanoids, eicosanoid synthesis was not increased in CLA compared to LA. Our results was suggest CLA has a possibility to protect the progress of atherosclerosis because CLA does not produce lipid production and endothelial contraction factors in liver.


  • 주제어

    conjugated linoleic acid (CLA) .   HepG2 cell .   lipid peroxidation .   prostagladin $E_2 (PGE_2) .   thromboxane A_2 (TXA_2)$.  

  • 참고문헌 (39)

    1. Dietary polyunsaturated fat modifies low-density lipoproteins and reduces atherosclerosis of non human primates with high and low diet responsiveness , Rudel,L.L.;Johnson,F.L.;Sawyer,J.K.;Wilson,M.S.;Parks,J.S. , Am J Clin Nutr / v.62,pp.463S-470S,
    2. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid , Ha,Y.L.;Grimm,N.K.;Pariza,M.W. , Carcinogenesis / v.8,pp.1881-1887,
    3. Fatty acid composition of low-density lipoprotein influences its susceptibility to autoxidation , Thomas,M.J.;Thoruburg,T.;Manning,J.;Hooper,K.;Rudel,L.L. , Biochemistry / v.33,pp.1828-1834,
    4. National Statistical Office , Causes of death startistics yearbook / v.,pp.,
    5. Eicosanoid synthesis in 7,12-dimethylbenz(a) anthracene-induced mammary adenocarci-nomas in Sprague-Dawley rats fed primrose oil, menhaden oil or corn oil diet , Abou-EI-Ela,S.H.;Prasse,K.W.;Carroll,R.;Wade,A.E.;Dharwadkar,S.;Bounce,O.R. , Lipid / v.23,pp.948-954,
    6. Effect of oleate-rich and linoleate-rich diets on the susceptibility of low density lipoprotein to oxidative modification in mildly hypercholesterolemic subjects , Reaven,P.;Parthasarathy,S.;Grasse,B.J.;Milleer,E.;Steinberg,D.;Witztum,J.L. , J Clin Invest / v.91,pp.668-676,
    7. Detection of Oxidized Low-Density Lipoproteins.Using Surface Plasmon Responce , Katharina,G.;Elizabeth,A.H.H. , Anal Chem / v.71,pp.2459-2467,
    8. Effect of different sources of dietary n-3 fatty acids on immune response and eicosanoids production , Kim,W.K. , J Korean Soc Fooc Sci Nutr / v.26,pp.697-703,
         
    9. Anticarcinogenic conjugated derivatives of linoleic acid found in grilled ground beef: isolation, identification, and mechanism of action , Ha,Y.L.;Pariza,M.W. , First Korean Conference on Science and Technology / v.,pp.442,
    10. Dietary fat and cancer risk: evidence and research needs , Pariza,M.W. , Annu Rev Nutr / v.8,pp.167-183,
    11. Role of oxygen free radicals in cancer development , Dreher,D.;Junod,A.F. , Eur J Cancer / v.32A,pp.30-38,
    12. Conjugated linoleic acid and oxidative stress , Banni,S.;Angioni,E.;Contini,M.S.;Carta,G.;Casu,V.;Iengo,G.A.;Melis,M.P.;Dians,M.;Dessi,M.A.;Corongiu,F.P. , J Am Oil Chem Soc / v.75,pp.261-267,
    13. Lee,M.S. , Apolipoprotein Metabolism / v.,pp.265-295,
    14. Antiplatelet effects of conjugated linoleic acid isomers , Truitt,A.;McNeil,G.;Vanderhoek,J.Y. , Biochim Biophys Acta / v.1438,pp.239-246,
    15. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines , Scudiero,D.A.;Shoemaker,R.H.;Paull,K.D.;Monks,A.;Tierney,S.;Nofziger,T.H.;Currens,M.J.;Seniff,D.;Boyd,M.R. , Cancer Res / v.48,pp.4827-4833,
    16. Dietary monounsaturaated fatty acid promote aortic atherosclerosis in LDL receptor-null, human ApoB100-overexpressing transgenicmice , Rudel,L.L.;Kelley,K.;Sawyer,J.K;Shah,R.;Wilson,Md , Arterioscler Throm Vasc Biol / v.18,pp.1818-1827,
    17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding , Bradford,M.M. , Anal Biochem / v.72,pp.248-254,
    18. Reinvestigation of the antioxidant properties of conjugated linoleic acid , van den Berg,J.J.;Cook,N.E.;Tribble,D.L. , Lipids / v.30,pp.599-605,
    19. Palmitic acid and linoleic acid metabolism in Caco-2 cells: different triglyceride synthesis and lipoprotein secretion , van Greevenbroek,M.M.;Voorhout,W.F.;Erkelens,D.W.;van Meer,G.;de Bruin,T.W. , J Lipid Res / v.36,pp.13-24,
    20. The growth inhibitory effect of conjugated linoleic acid on MCF-7 cells is released to estrogen response system , Durgam,V.R.;Fernandes,G. , Cancer Lett / v.116,pp.121-130,
    21. Activities of phospholipase A2 and cyclooxygenase, and syntheses of thromboxane and prostacyclin in streptozotocin induced diabetic rats , Yang,J.A.;Kim,S.O.;Choi,J.H.;Kwag,O.G.;Rhee,S.J.;Chang,H.W. , J Korean Soc Fooc Nutr / v.27,pp.175-181,
         
    22. Dietary conjugated linoleic acids promote fatty streak formation in the C57BL/6 mouse atherosclosis model , Munday,J.S.;Thompson,K.G.;James,K.A. , Br J Nutr / v.81,pp.251-255,
    23. A simple method for the isolation and punification of total lipids from animal tissues , Folch,J.;Lees,M.;Sloane Stanley,G.H. , J Biol Chem / v.226,pp.497-509,
    24. The Growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cells , Igrashi,M.;Miyazawa,T. , Biochim Biophys Acta / v.1530,pp.162-171,
    25. Hepatic orgin or cholesteryl oleate in cononary artery atherosclerosis in Arican green monkeys. Enrichment by dietary monounsaturated fat , Rudel,L.L.;Haines,J.;Sawyer,J.K.;Shah,R.;Wilson,M.S.;Carr,T.P. , J Clin Invest / v.100,pp.74-83,
    26. Conjugated linoleic acid and atherosclerosis in rabbits , Lee,K.N.;Kritchevsky,D.;Pariza,M.W. , Atherosclerosis / v.108,pp.19-25,
    27. Effect of dietary conjugated linoleic acid on phorbol ester-induced PGE2 production and hyperplasia in mouse epidermis , Kavanaugh,C.J.;Liu,K.L.;Belury,M.A. , Nutrition and Cancer / v.33,pp.132-138,
    28. Korean status of alcolouics and alcohol related health problems , Park,S.C.;Oh,S.I.;Lee,M.S. , Alcohol Clin Exp Res / v.22,pp.170S-172S,
    29. Reinvestigation of the antioxidant properties of conjugated linoleic acid , Van den Berg,J.J.;Cook,N.E.;Tribble,D.L. , Lipid / v.30,pp.599-605,
    30. Proliferative responses of normal human mammary and MCF-7 breast cancer cells to linoleic acid, conjugated linoleic acid and eicosanoid synthesis inhibitors in culture , Cunningham,D.C.;Harrison,L.Y.;Shultz,T.D. , Anticancer Res / v.17,pp.197-203,
    31. Diet and heart: a postscript , Morris,J.N.;Marr,J.W.;Clayton,D.G. , Br Med J / v.2,pp.1307-1314,
    32. Inducible isoforms of cyclooxygenase and nitric oxide synthase in inflammation , Vane,J.R.;Mitchell,J.A.;Appleton,I.;Tomlinson,A.;Bailey,D.B.;Croxtall,J.;Willoughby,D.A. , Proc Natl Acad Sci USA / v.91,pp.2046-2050,
    33. Metabolites of conjugated isomers of linoleic acid (CLA) in the rat , Sebedio,J.L.;Juaneda,P.;Dobson,G.;Ramilison,I.;Martin,J.C.;Chardigny,J.M.;Christie,W.W. , Biochim Biophys Acta / v.1345,pp.5-10,
    34. Dietary conjugated linoleic acid reduces plasma lipoproteins and ealy aotic atherosclerosis in hypercholesterolemic hamsters , Nicolosi,R.J.;Rogers,E.J.;Kritchevsky,D.;Scimeca,J.A.;Huth,P.J. , Artery / v.22,pp.266-277,
    35. Requirement of esscential fatty acid for mammary tumorigenesis in the rat , Ip,C.;Carter,C.A.;Ip,M.M. , Cancer Res / v.45,pp.1999-2001,
    36. Inhibition of benzo(a)pyreneinduced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid , Ha,Y.L.;Storkson,J.;Pariza,M.W. , Cancer Res / v.50,pp.1097-1101,
    37. Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratin ocytes , Kai-Li Liu;Martha A. Belury , Cancer Letters / v.127,pp.15-22,
    38. Ekhar,E.Z.;L.J.Filer,Jr. , Present knowledge in nutrition(7th Edition) / v.,pp.,
    39. Dietary polyunsaturated fat decreases coronary artery atherosclerosis in a pediatric-aged population of African green monkeys , Wolfe,M.S.;Sawyer,J.K.;Morgan,T.M.;Bullock,B.D.;Rudel.L.L. , Arterioscler Thromb / v.14,pp.587-597,

 저자의 다른 논문

  • 문희정 (1)

    1. 2003 "식이성 Conjugated Linoleic Acid (CLA)가 유선암 세포(MCF-7)에서의 항암효과에 미치는 영향" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 32 (3): 418~427    
  • Lee, Myoung-Sook (25)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기