본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

미성숙 돼지 난자의 유리화 동결에 관한 연구: Open Pulled Straw(OPS), Electron Microscopic Grid(EMG) 및 Nylon Loop System(NLS)의 비교
Study of Vitrification of Immatured Pig Oocytes: Compared with Open Pulled Straw(OPS), Electron Microscopic Grid(EMG) and Nylon Loop System(NLS)

김인덕   (단국대학교 생명자원과학대학 동물자원과학과UU0000336  ); 안미현   (단국대학교 생명자원과학대학 동물자원과학UU0000336  ); 석호봉   (단국대학교 생명자원과학대학 동물자원과학과UU0000336  );
  • 초록

    This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.


  • 주제어

    porcine oocytes .   vitrification .   open pulled straw .   EM grid .   nylon loop).  

  • 참고문헌 (26)

    1. Beebe FS, Cameron RDA, Blackshaw AW, Higgins A and Nottle MB. 2002. Piglets born from vitrified zona-intact blastocysts, Theriogenology, 57:2155-2165 
    2. Kim MK, Lee SJ, Uhm EY, Yoon SH, Park SP, Chung KS and Lim JH. 1996. Cryopreservation of mouse IVF zygotes by vitrification. Korean J. Animal Reprod., 20:119-126 
    3. Lane M, Schoolcraft WB and Gardner DK. 1999b. Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique, Fertil Steril. 72:1073-1078 
    4. Nagashima H, Kashiwazaki N, Ashman RJ, Grupen CG, Seamark RF and Nottle MB. 1994. Removal of cryoplasmic lipid enhances the tolerance of porcine embryos to chilling. Biol. Reprod., 51:618-622 
    5. Pollard JM and Leibo SP. 1994. Chilling sensitivity of mammalian embryos. Theriogenology, 41: 101-106 
    6. Rall WF and Fahy GM. 1985. Ice-free cryopreservation of mouse embryos at $-196^{\circ}C$ by vitrification. Nature, 313:573-575 
    7. Rayos AA, Takahashi Y, Hishinuma M and Kanagawa H. 1994. Quick freezing of unfertilized mouse oocytes using ethylene glycol with sucrose or trehalose. J. Reprod. Fertil., 100:123-129 
    8. Schellander K, Peli J, Schmoll F and Brem G. 1994. Effects of cryopreservation and carbohydrates on freezing of matured and unmatured bovine oocytes. Theriogenology, 23:909-915 
    9. Vajta G, Holm P, Greve T and Callesen H. 1997b. Vitrification of porcine embryos using open pulled straw method. Acta. Vet. Scand., 38: 349-352 
    10. Vajta G, Lewis IM, Kuwayama M, Greve T and Callesen H. 1998b. Sterile application of the open pulled straw(OPS) vitrification method. Cryo-Letters, 19:389-392 
    11. Van der Elst J, Vanden Abbeel ER, Jacobs E and Van Steirtghem A. 1988. Effect of 1,2-propaneiol and dimethylsulphoxide on the meiotic spindle of the mouse oocyte. Human Reprod., 3:960-967 
    12. Lewis IM, Lane MW and Vajta G. 1999. Pregnancy rate following transfer of in vitro produced bovine embryos vitrified by the open pulled straw(OPS) method. Theriogenology, 51: 168(Abstr.) 
    13. Park SP, Kim EY, Kim DI, Park NH, Won YS, Yoon SH, Chung KS and Lim JH. 1999. Simple, efficient and successful vitrification of bovine blastocysts using electron microscope grids. Human Reprod., 14:2838-2843 
    14. Volodmir I, Carles S, Eugenia I, Francisco PS and Valentin G. 1998. Vitrification of immature porcine oocyte: Effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant. Cryobiology, 36:250-253 
    15. Martino A, Songsasen N and Leibo SP. 1996. Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol. Reprod., 54:1059-1069 
    16. Vajta G, Booth PJ, Holm P, Greve T and callesen H. 1997a. Successful vitrification of early stage bovine in vitro produced embryos with the open pulled straw(OPS) method. Cryo-Letters, 18:191-195 
    17. Mahmoudzadeh AR, van Soom A, Ysebaert MT and De Kruif A. 1994. Comparison of two-step vitrification versus controlled freezing on survival of in vitro produced cattle embryos. Theriogenology, 42:1389-1397 
    18. Hotamisligil S, Toner M and Power R. 1996. Change in membrane integrity, cytoskeletal structure and developmental potential of murine oocytes after vitrification in ethylene glycol. Biol. Reprod., 55:161-168 
    19. Whittingham DG, Leibo SP and Mazur P. 1972. Survival of mouse embryos frozon to -196$^{\circ}C$ an -269$^{\circ}C$. Science, 178:411-414 
    20. Lane M, Bavister BD, Lyons EA and Forest KT. 1999a. Containerless vitrification of mammalian oocytes and embryos. Nat. Biotechnol., 17: 1234-1236 
    21. Pieterse MC, Vos PLAM, Kruip ThAM, Wurth YA, van Benden ThA, Willemse AH and Taverne MAM. 1991. Transvaginal ultrasound guided follicular aspiration of bovine oocyte. Theriogenology, 53:19-24 
    22. Quinn P, Kerin J, Stone B and Wilson L. 1986. Successful cryopreservation of human oocytes. In: 42nd Ann Mtg Am Fertil Soc and 18th Ann Mtg Canadian Fertil Androl Soc Abstr, 72 (Abst) 
    23. Choi IK, Lee SJ and Song HB. 2003. Study on the vitrification of porcine GV and MⅡ oocytes after removal of cytoplasmic lipid droplets. Korean J. Embryo Transfer, 17:1-14 
    24. Pickering SJ and Johnson HM. 1987. The influenced of cooling on the organozation of the meiotic spindle of the mouse oocyte. Human Reprod., 2:207-216 
    25. Nagashima H, Kuwayama M, Grupen CG, Ashman RJ and Nottle MB. 1996. Vitrification of porcine early cleavage stage embryos and oocytes after removal of cytoplasmic lipid droplets. Theriogenology, 45(1):180 
    26. Lane M and Gardner DK. 2001. Vitrification of mouse oocytes using a nylon loop. Mol. Reprod. Dev., 58:342-347 
  • 이 논문을 인용한 문헌 (1)

    1. Lee, Hae-Lee ; Kim, Sang-Hun ; Kim, Yong-Jun 2014. "Studies on Cryotop Vitrification Method for Simple Freezing of Hanwoo Embryos" Journal of embryo transfer = 한국수정란이식학회지, 29(1): 13~19     

 저자의 다른 논문

  • 김인덕 (4)

    1. 2004 "돼지 배아의 유리화 동결 시 Cytochalasin B의 농도와 처리 시간에 의한 효과" 韓國受精卵移植學會誌 = Korean journal of embryo transfer 19 (1): 35~42    
    2. 2004 "PVA, PVP 및 pFF를 첨가한 체외성숙 한정배지가 미성숙 돼지 난포란의 성숙과 배발달에 미치는 영향" 韓國受精卵移植學會誌 = Korean journal of embryo transfer 19 (3): 219~227    
    3. 2004 "Open Pulled Straw(OPS) 방법에 의한 체외배양 동결수정란의 경산돈 이식 : 예비실험 결과" 韓國受精卵移植學會誌 = Korean journal of embryo transfer 19 (2): 155~163    
    4. 2008 "Open Pulled Straw(OPS) 방법에 의한 체외 배양 동결 수정란의 미경산돈 이식" Journal of embryo transfer = 한국수정란이식학회지 23 (3): 217~222    
  • Ahn, Mi-Hyun (3)

  • 석호봉 (25)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기