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Indicator-free DNA Chip Array Using an Electrochemical System

Park, Yong-Sung   (School of Electrical, Electronic and Information Engineering, Wonkwang UniversityUU0001021  ); Kwon, Young-Soo   (Div. of Electrical, Electronic and Computer Eng., Dong-A Universit  ); Park, Dae-Hee   (School of Electrical, Electronic and Information Engineering, Wonkwang UniversityUU0001021  );
  • 초록

    This research aims to develop a DNA chip array without an indicator. We fabricated a microelectrode array through photolithography technology. Several DNA probes were immobilized on an electrode. Then, target DNA was hybridized and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between the DNA probe and mismatched DNA in an anodic peak. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.


  • 주제어

    DNA probe .   Hybridization .   Indicator-free DNA chip .   Immobilization .   Target DNA.  

  • 참고문헌 (12)

    1. D. K. Kim, Y. S. Choi, Y. Murakami, E. Tamiya and Y. S. Kwon, 'Real-Time Detection of DNA Hybridization Assay by using Evanescent Field Microscopy', KIEE Inter. Trans. on EA, Vol. 11C, No.3, pp. 85-90, 2001 
    2. E. Palecek, M. Fojta, M. Tomschik and J. Wang, 'Electrochemical biosensors for DNA hybridization and DNA damage', Biosens. Bioelectron., Vol. 13, pp. 621-628, 1998 
    3. D. H. Johnston, K. C. Glasgow, and H. H. Thorp, 'Electrochemical Measurement of the Solvent Accessibility of Nucleobases Using Electron Transfer between DNA and Metal Complexes', J. Arn. Chern.Soc., Vol. 117, pp. 8933-8938,1995 
    4. M. I. Pividori, A. Merko and S. Alegret, 'Electrochemical genosensor design: immobilisation of oligonucleotides onto transducer surfaces and detection methods', Biosens. Bioelectron., Vol. 15, pp. 291-303, 2000 
    5. M. Schena, D. Shalon, R. Heller, A. Chai, P.O. Brown, R.W. Davis,'Parallel human genome analysis: microarray-based expression monitoring of 1000 genes', Proc. Natl. Acad. Sci. USA, Vol. 93, pp.10614-10619, 1996 
    6. Y. S. Choi, D. K. Kim and Y. S. Kwon,'Highly Integrated DNA Chip Microarrays by Hydrophobic Interaction', KIEE Inter. Trans. on EA, Vol. 11C, No. 2, pp. 23-27, 2001 
    7. E. M. Southern, S. C. Case-Green, J. K. Elder, M. Johnson, K. U. Mir, L. Wang and J. C. Williams, 'Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids', Nucleic Acids Res., Vol. 22, pp. 1368-1373, 1994 
    8. M. J. O'Donnell, K. Tang, H. Koster, C. L. Smith, and C. R. Cantor,'High-Density, Covalent Attachment of DNA to Silicon Wafers for Analysis by MALDI-TOF Mass Spectrometry', Anal. Chern., Vol. 69, pp. 2438-2443, 1997 
    9. Y. S. Choi, D. H. Park, Y. S. Kwon and T. Tomoji, 'Development of New DNA Chip and Genome Detection Using an Indicator-free Target DNA',Trans. KIEE, Vol. 52C, No.8, pp. 365-370, 2003 
    10. A. C, Pease, D. Solas, E. J. Sullivan, M. T. Cronin, C. P. Holmes and S. P. A. Fodor,'Light-Generated Oligonucleotide Arrays for Rapid DNA Sequence Analysis', Proc. Natl. Acad. of Sci. U.S.A., Vol. 91, pp. 5022-5026, 1994 
    11. A. B. Steel, T. M. Herne, and M. J. Tarlov, 'Electrochemical Quantitation of DNA Immobilized on Gold', Anal. Chern., Vol. 70, pp. 4670-4677, 1998 
    12. M. Schena, D. Shalon, R.W. Davis, P.O. Brown, 'Quantitative monitoring of gene expression patterns with a complementary DNA microarray', Science, Vol.270, pp.467-470, 1995 

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