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Determination of Flavonoids, Tannins and Ellagic Acid in Leaves from Rubus L. Species

Gudej, Jan    (Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul., Department of Pharmacognosy, Faculty of Pharmacy, Medical University of dz, ul.   ); Tomczyk, Michal    (Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul.  );
  • 초록

    This paper describes the quantitative determination of flavonoids, tannins and ellagic acid in the leaves from wild and cultivated variations of Rubus L. species (Rosaceae): raspberry (2 wild and 13 cultivars) and blackberry (3 wild and 3 cultivars). The content of flavonoids was analyzed using spectrophotometric (the Christ-M llers method) and HPLC analysis after acid hydrolysis. The content of tannins was determined by the weight method, with hide powder, described by German Pharmacopoeia 10 (DAB 10). Ellagic acid content was examined using the HPLC method after acid hydrolysis. Flavonoid content, determined using the Christ-Muller's method was higher for the blackberry leaves than for the raspberry leaves and varied between 0.46% and 1.05%. Quercetin and kaempferol were predominant in all samples analyzed using the HPLC method. The highest flavonoid content was found in the leaves of R. nessensis (1.06%); with results in all of the examined samples varying between 0.27% and 1.06%. The concentration of ellagic acid in all species was determined after acid hydrolysis and ranged from 2.06% to 6.89%. The leaves of raspberries are characterized by greater amounts of tannins (varying between 2.62% and 6.87%) than the leaves of other species. The results from this study indicate that the analyzed species are a rich source of flavonoids, ellagic acid and tannins, which may be used for the quality assessment of Rubus L. species leaves.


  • 주제어

    Rubus L.   species .   Flavonoids .   Tannins .   Ellagic acid .   HPLC determination .   Spectrophotometric determination.  

  • 참고문헌 (36)

    1. Hakkinen, S. H., Karenlarnpi, S. O., Mykkanen, H. M., Heinonen, I. M., and Torronen, A. R., Ellagic acid content in berries: influence of domestic processing and storage. Eur. Food Res. Technol., 212, 75-80 (2000) 
    2. Marczal, G., Qualitative studies of the tannin content of Rubus idaeus leaf. Herb. Hung., 2, 347-357 (1963) 
    3. Mullen, W., McGinn, J., Lean, M. E. J., MacLean, M. R., Gardner, P., Duthie, G. G., Yokota, T., and Crozier, A., Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties. J. Agric. Food Chem., 50, 5191-5196 (2002) 
    4. Priyadarsini, K. I., Khopde, S. M., Kumar, S. S., and Mohan, H., Free radical studies of ellagic acid, a natural phenolic antioxidant. J. Agric. Food Chem., 50, 2200-2206 (2002) 
    5. Simpson, M., Parsons, M., Greenwood, J., and Wade, K., Raspberry leaf in pregnancy: its safety and efficacy in labor. J. Midwifery Womens Health, 46, 51-59 (2001) 
    6. Wojcik, E., Phytochemical investigation of Rubus plicatus blackberry. Acta Polon. Pharm., 46, 386-390 (1989) 
    7. Daniel, E. M., Krupnick, A. S., Heur, Y. H., Blinzler, J. A., Nims, R. W., and Stoner, G. D., Extraction, stability and quantitation of ellagic acid in various fruits and nuts. J. Food Comp. Anal., 2, 338-349 (1989) 
    8. Gwozdecki, J., Zeszyty pomologiczne, and Malina i Jezyna. Instytutu Sadownictwa i Kwiaciarstwa, Skierniewice pp. 5-53 (1996) 
    9. Shepherd, T., Robertson, G. W., Griffiths, D. W., and Birch, A. N. E., Epicuticular wax ester and triacylglycerol composition in relation to aphid infestation and resistance in red raspberry (Rubus idaeus L.). Phytochemistry, 50, 1255-1267 (1999) 
    10. Amakura, Y., Okada, M., Tsuji, S., and Tonogai, Y., High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. J. Chromatogr. A, 896, 87-93 (2000) 
    11. Burn, J. H. and Withell, E. R., A principle in raspberry leaves which relaxes uterine muscle. Lancet, 5, 1-3 (1941) 
    12. Barch, D. H., Rundhaugen, L. M., Stoner, G. D., Pillay, N. S., and Rosche, W. A., Structure-function relationships of the dietary anticarcinogen ellagic acid. Carcinogenesis, 17, 265-269 (1996) 
    13. Czygan, F. Ch., Die Himbeere-Rubus idaeus L. Portrait einer Arzneipflanze. Z. Phytother., 16, 366-374 (1995) 
    14. Robbers, J. E., and Tyler, V. E., In: Tyler's Herbs of choice: the therapeutic use of phytomedicinals, The Haworth Herbal Press, New York-London pp. 63-64, 194-195 (1999) 
    15. Christ, B. and Muller, K. H., Determination of the amount of flavonol derivatives in drugs. Arch. Pharm., 293, 1033-1042 (1960) 
    16. Rommel, A. and Wrolstad, R. E., Ellagic acid content of red raspberry juice as influenced by cultivar, processing and environmental factors. J. Agric. Food Chem., 41, 1951-1960 (1993) 
    17. Thiem, B. and Gooslinska, O., Antimicrobial activity of Rubus chamaemorus leaves. Fitoterapia, 75, 93-95 (2004) 
    18. Rojas-Vera, J., Patel, A. V., and Dacke, C. G., Relaxant activity of raspberry (Rubus idaeus) leaf extract in guinea-pig ileum in vitro. Phytother. Res., 16, 665-668 (2002) 
    19. Alice, L. A. and Campbell, Ch. S., Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. Am. J. Bot., 86, 81-97 (1999) 
    20. Borkowski, B., Lutomski, J., Skrzydlewska, E., and Zygmunt, B., Rosliny lecznicze w fitoterapii, IRiPZ, Poznan pp. 470-471(1994) 
    21. Krawczyk, A., Thiem, B., and Szkudlarek, M., High-performance liquid chromatography of ellagic acid from the leaves of Rubus chamaemorus L. Chem. Anal. (Warsaw), 48, 891-899 (2003) 
    22. Tomczyk, M. and Gudej, J., Quantitative analysis of flavonoids in the flowers and leaves of Ficaria verna Huds. Z. Naturforsch., 58c, 762-764 (2003) 
    23. Hakkinen, S., Heinonen, M., Karenlampi, S., Mykkanen, H., Ruuskanen, J., and Torronen, R., Screening of selected flavonoids and phenolic acids in 19 berries. Food Res. Int., 32, 345-353 (1999) 
    24. Olszewska, M., Glowacki, R., Wolbioe, M., and Bald, E., Quantitative determination of flavonoids in the flowers and leaves of Prunus spinosa L. Acta Polan. Pharm., 58, 199-203 (2001) 
    25. Polish Pharmacopoeia VI, Polish Pharmaceutical Society(eds.), Warszawa p. 150 (2002) 
    26. Heslop-Harrison, Y., Rubus L. In: Tutin TG et al. (eds.) Flora Europaea. Vol. II, Cambridge University Press p. 7-25 (1968) 
    27. Okuda, T., Yoshida, T., Hatano, T., Iwasaki, M., Kubo, M., Orime, T., Yoshizaki, M., and Naruhashi, N., Hydrolysable tannins as chemotaxonomic markers in the Rosaceae. Phytochemistry, 31, 3091-3096 (1992) 
    28. Gudej, J., Kaempferol and quercetin glycosides from Rubus idaeus L. leaves. Acta Polon. Pharm., 60, 313-316 (2003) 
    29. Sticher, O., Quality of Ginkgo preparations. Planta Med., 59, 2-11(1993) 
    30. Gudej, J. and Rychlinska, I., Flavonoid compounds from the leaves of Rubus idaeus L. Herba Pol., 42, 257-261 (1996) 
    31. Gudej, J., Tomczyk, M., Urban, E., and Tomczykowa, M., Analysis of chemical composition of Rubus saxatilis L. leaves. Herba Pol., 44, 340-344 (1998) 
    32. DAB 10 Deutsches Arzeneibuch, Amtliche Ausgabe, Deutcher Apotheker Verlag, Stuttgart p. 197 (1998) 
    33. Havsteen, B., The biochemistry and medical significance of flavonoids. Pharmacol. Therap., 96, 67-202 (2002) 
    34. Patel, A. V., Rojas-Vera, J., and Dacke, C. G., Therapeutic constituents and actions of Rubus species. Curr. Med. Chem., 11, 1501-1512 (2004) 
    35. Krzaczek, T., Phenolic acids in some tannin drugs of the Rosaceae family. Farm. Pol., 40, 475-477 (1984) 
    36. Ozarowski, A., and Jaroniewski, W., Rooeliny lecznicze i ich praktyczne zastosowanie, IWZZ, Warszawa pp. 184, 243 (1989) 
  • 이 논문을 인용한 문헌 (1)

    1. Na, Hwan Sik ; Kim, Jin Young ; Yun, Seol Hee ; Park, Hak Jae ; Choi, Gyeong Cheol ; Yang, Soo In ; Lee, Ji Heon ; Cho, Jeong Young 2013. "Phytochemical contents of agricultural products cultivated by region" 한국식품저장유통학회지 = Korean journal of food preservation, 20(4): 451~458     

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