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Determination of Flavonoids, Tannins and Ellagic Acid in Leaves from Rubus L. Species

Gudej, Jan    (Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul., Department of Pharmacognosy, Faculty of Pharmacy, Medical University of dz, ul.   ); Tomczyk, Michal    (Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul.  );
  • 초록

    This paper describes the quantitative determination of flavonoids, tannins and ellagic acid in the leaves from wild and cultivated variations of Rubus L. species (Rosaceae): raspberry (2 wild and 13 cultivars) and blackberry (3 wild and 3 cultivars). The content of flavonoids was analyzed using spectrophotometric (the Christ-M llers method) and HPLC analysis after acid hydrolysis. The content of tannins was determined by the weight method, with hide powder, described by German Pharmacopoeia 10 (DAB 10). Ellagic acid content was examined using the HPLC method after acid hydrolysis. Flavonoid content, determined using the Christ-Muller's method was higher for the blackberry leaves than for the raspberry leaves and varied between 0.46% and 1.05%. Quercetin and kaempferol were predominant in all samples analyzed using the HPLC method. The highest flavonoid content was found in the leaves of R. nessensis (1.06%); with results in all of the examined samples varying between 0.27% and 1.06%. The concentration of ellagic acid in all species was determined after acid hydrolysis and ranged from 2.06% to 6.89%. The leaves of raspberries are characterized by greater amounts of tannins (varying between 2.62% and 6.87%) than the leaves of other species. The results from this study indicate that the analyzed species are a rich source of flavonoids, ellagic acid and tannins, which may be used for the quality assessment of Rubus L. species leaves.

  • 주제어

    Rubus L.   species .   Flavonoids .   Tannins .   Ellagic acid .   HPLC determination .   Spectrophotometric determination.  

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  • 이 논문을 인용한 문헌 (1)

    1. Na, Hwan Sik ; Kim, Jin Young ; Yun, Seol Hee ; Park, Hak Jae ; Choi, Gyeong Cheol ; Yang, Soo In ; Lee, Ji Heon ; Cho, Jeong Young 2013. "Phytochemical contents of agricultural products cultivated by region" 한국식품저장유통학회지 = Korean journal of food preservation, 20(4): 451~458     

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