본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Inhibition of Trypsin-Induced Mast Cell Activation by Water Fraction of Lonicera japonica

Kang, Ok-Hwa    (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University   ); Choi, Yeon-A    (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University   ); Park, Hye-Jung    (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University   ); Lee, Joo-Young    (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University   ); Kim, Dae-Ki    (Department of Oriental Pharmacy, College of Pharmacy   ); Choi, Suck-Chei    ( Wonkwang University   ); Kim, Tae-Hyun    (Department of Oriental Pharmacy, College of Pharmacy   ); Nah, Yong-Ho    ( Wonkwang University   ); Yun, Ki-Jung    (Department of Immunology, Chonbuk National University Medical School   ); Choi, Suck-Jun    (Institute of Digestive Disease, Wonkwang University School of Medicine   ); Kim, Young-Ho    (College of Pharmacy, Chungnam National University   ); Bae, Ki-Hwan    (College of Pharmacy, Chungnam National University   ); Lee, Young-Ml    (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University  );
  • 초록

    Lonicera japonica Thunb.(Caprifoliaceae) has long been known as an anti-inflammatory. In the present study, the effect of water fraction of Lonicera japonica (LJ) on trypsin-induced mast cell activation was examined. HMC-1 cells were stimulated with trypsin (100 nM) in the presence or absence of LJ (10, 100, and 1000 $\mu$ g/mL). TNF- $\alpha$ and tryptase production were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-PCR. Extracellular signal-regulated kinase (ERK) phosphorylation was assessed by Western blot. Trypsin activity was measured by using Bz-DL-Arg-p-nitroanilide (BAPNA) as substrate. LJ (10, 100, and 1000 $\mu$ g/mL) inhibited TNF- $\alpha$ secretion in a dose-dependent manner. LJ (10, 100, and 1000 $\mu$ g/mL) also inhibited TNF- $\alpha$ and tryptase mRNA expression in trypsin-stimulated HMC-1. Furthermore, LJ inhibited trypsin-induced ERK phosphorylation. However, LJ did not affect the trypsin activity even 1000 $\mu$ g/mL. These results indicate that LJ may inhibit trypsin-induced mast cell activation through the inhibition of ERK phosphorylation than the inhibition of trypsin activity.


  • 주제어

    Lonicera japonica .   Trypsin .   Tryptase .   TNF- $\alpha$ .   ERK.  

  • 참고문헌 (22)

    1. Cenac, N., Coelho, A. M., Nguyen, C., Compton, S., Andrade-Gordon, P., MacNaughton, W. K., Wallace, J. L., Hollenberg, M. D., Bunnell, N. W., Garcia-Villar, R., Bueno, L., and Vergnolle, N., Induction of intestinal inflammation in mouse by activation of proteinase-activated receptor-2. Am. J.Pathol., 161, 1903-1915 (2002) 
    2. Raithel, M., Winterkamp, S., Pacurar, A, Ulrich, P., Hochberger, J., and Hahn, E. G., Release of mast cell tryptase from human colorectal mucosa in inflammatory bowel disease. Scand. J. Gastroenterol., 36,174-179 (2001) 
    3. Souza Pinto, J. C., Remacle-Volon, G., Sampaio, C. A., and Damas, J., Collagenase-induced oedema in the rat paw and the kinin system. Eur. J. Pharmacol., 274,101-107 (1995) 
    4. Tae, J., Han, S. W., Yoo, J. Y., Kim, J. A., Kang, O. H., Baek, O. S., Lim, J. P., Kim, D. K., Kim,Y. H., Bae, K. H., and Lee, Y. M., Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema. Clin. Chim. Acta., 330,165-171 (2003) 
    5. Lee, J. H., Ko, W. S., Kim, V. H., Kang, H. S., Kim, H. D., and Choi, B. T., Anti-inflammator effect of the aqueous extract from Lonicera japonica flower is related to inhibition of $NF-{\kappa}B$ activation through reducing $l-{\kappa}B{\alpha}$ degradation in rat liver. Int. J. Mol. Med., 7, 79-83 (2001) 
    6. Kim, J. A., Choi, S. C., Yun, K. J., Kim, D. K., Han, M. K., Seo, G. S., Yeom, J. J., Kim, T. H., Nah, V. H., and Lee, V. M., Expression of protease-activated receptor 2 in ulcerative colitis. Inflamm. Bowel. Dis., 9, 224-249 (2003) 
    7. Gratz, R., Becker, S., Sokolowski, N., Schumann, M., Bass, D., and Malnick, S. D., Murine monoclonal anti-TNF antibody administration has a beneficial effect on inflammatory bowel disease that develops in IL-10 knockout mice. Dig. Dis. Sci., 47, 1723-1727 (2002) 
    8. Lee, Y. B., Schrader, J. W., and Kim, S. U., P38 map kinase regulates TNF-alpha production in human astrocytes and microglia by multiple mechanisms. Cytokine., 12, 874-880 (2000) 
    9. Lundberg, A. H., Eubanks, J. W. 3rd., Henry, J., Sabek, O., Kotb, M., Gaber, L., Norby-Teglund, A, and Gaber, A. O., Trypsin stimulates production of cytokines from peritoneal macrophages in vitro and in vivo. Pancreas, 21, 41-51 (2000) 
    10. Kang, O. H., Jeong, H. J., Kim, D. K., Choi. S. C., Kim, T. H., Nah, Y. H., Kim, H. M., and Lee, V. M., Trypsin induces tumour necrosis factor-alpha secretion from a human leukemic mast cell line. Cell Biochem. Funct., 21, 161-167 (2003) 
    11. Wang, Z. Y., Clinical and laboratory studies of the effect of an antilupus pill on systemic lupus erythematosus. Zhong Xi YI Jie He Za Zhi, 9, 465-468 (1989) 
    12. Duan, W., Chan, J. H., Wong, C. H., Leung, B. P., and Wong, W. S., Anti-inflammatory effects of mitogen-activated protein kinase kinase inhibitor U0126 in an asthma mouse model. J. Immunol., 172, 7053-7059 (2004) 
    13. Araki, Y., Andoh, A., Fujiyama, Y., and Bamba,T., Development of dextran sulphate sodium induced experimental colitis is suppressed in genetically mast cell-deficient Ws/Ws rats. Clin. Exp. Immunol., 119, 264-269 (2000) 
    14. Ishizuka, T., Terada, N., Gerwins, P., Hamelmann, E., Oshiba, A., Fanger, G. R., Johnson, G. L., and Gelfand, E. W., Mast cell tumor necrosis factor alpha production is regulated by MEK kinases. Proc. Natl. Acad. Sci. U.S.A., 94, 6358-6363 (1997) 
    15. Murakami, A., Nakamura, Y., Ohto, Y., Yano, M., Koshiba, T., Koshimizu, K., Tokuda, H., Nishino, H., and Ohigashi, H., Suppressive effects of citrus fruits on free radical generation and nobiletin, an anti-inflammatory polymethoxyflavonoid. Biofactors, 12, 187-192 (2000) 
    16. Vanderslice, P., Ballinger, S. M., Tam, E. K., Goldstein, S. M., Craik, C. S., and Caughey, G. H., Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease family. Proc. Natl. Acad. Sci. U.S.A., 87, 3811-3815 (1990) 
    17. Tremaine, W. J., Brzezinski, A., Katz, J. A., Wolf, D. C., Fleming, T. J., Mordenti, J., Strenkoski-Nix, L. C., and Kurth, M. C., Treatment of mildly to moderately active ulcerative colitis with a tryptase inhibitor (APC 2059): an open-label pilot study. Aliment. Pharmacol. Ther.,16, 407-413(2002) 
    18. Kawabata, A., Kuroda, R., Nakaya, Y., Kawai, K., Nishikawa, H., and Kawao, N., Factor Xa evoked relaxation in rat aorta: involvement of PAR-2. Biochem. Biophys. Res. Commun., 282, 432-435 (2001) 
    19. Smith, T. J., Hougland, M. W., and Johnson, D. A., Human lung tryptase. Purification and characterization. J. Biol. Chem., 259,11046-11051 (1984) 
    20. Metcalfe, D. D., Baram, D., and Mekori, V. A., Mast cells. Physiol. Rev., 77,1033-1079 (1997) 
    21. Weiss, T., Shalit, I., Blau, H., Werber, S., Halperin, D., Levitov, A., and Fabian, I., Anti-inflammatory effects of moxifloxacin on activated human monocytic cells: inhibition of NF-kappaB and mitogen-activated protein kinase activation and of synthesis of proinflammatory cytokines. Antimicrob. Agents Chemother., 48,1974-1982 (2004) 
    22. Ware, J. H., Wan, X. S., Newbeme, P., and Kennedy, A. R., Bowman-Birk inhibitor concentrate reduces colon inflammation in mice with dextran sulfate sodium-induced ulcerative colitis. Dig. Dis. Sci., 44, 986-990 (1999) 
  • 이 논문을 인용한 문헌 (3)

    1. 2007. "" Journal of plant biology = 식물학회지, 50(3): 315~320     
    2. Kim, Yong ; Yang, Su-Young ; Oh, Young-Seon ; Lee, Jin-Woo ; Lee, Yong-Koo ; Park, Yang-Chun 2010. "Research trends of Lonicera japonica over the last 10 years" 論文集 : 大田大學校 韓醫學硏究所. 韓醫學編, 19(1): 17~23     
    3. 2011. "" Oriental pharmacy and experimental medicine : OPEM, 11(4): 287~292     

 저자의 다른 논문

  • 강옥화 (29)

    1. 2004 "Citrus unshiu Water Extract Inhibits Trypsin-induced $TNF-{\alpha}$ and Tryptase Productions by Blocking the ERK Phosphorylation and Trypsin Activity" Natural product sciences 10 (5): 211~216    
    2. 2005 "바꽃(烏頭)의 포제(抱製)에 관한 연구" 大韓韓醫學方劑學會誌 = Herbal formula science 13 (2): 141~151    
    3. 2005 "Inhibitory Effect of Tetragonia tetragonoides Water Extract on the Production of $TNF-{\alpha}$ and Tryptase in Trypsin-Stimulated Human Mast Cells" Natural product sciences 11 (4): 207~212    
    4. 2006 "시호(柴胡)의 승약작용에 관한 연구" 大韓本草學會誌 = The Korea journal of herbology 21 (4): 213~218    
    5. 2006 "십팔반(十八反) 에 관한 연구" 大韓韓醫學方劑學會誌 = Herbal formula science 14 (2): 1~8    
    6. 2007 "한약의 복용시간에 관한 고찰" 大韓本草學會誌 = The Korea journal of herbology 22 (4): 1~8    
    7. 2007 "십구외(十九畏)에 관한 고찰" 大韓韓醫學方劑學會誌 = Herbal formula science 15 (2): 9~19    
    8. 2007 "'세신불가전(細辛不過錢)'에 관한 연구" 大韓本草學會誌 = The Korea journal of herbology 22 (1): 49~52    
    9. 2007 "피휘(避諱)에 관한 연구" 大韓韓醫學方劑學會誌 = Herbal formula science 15 (1): 129~135    
    10. 2008 "Effect of Jak-Yak Tang water extract on expression of cytokin and chemokine" Journal of oriental pharmacy 1 (1): 1~5    
  • Choi, Yeon-A (3)

  • 박혜정 (9)

  • Choi, Suck-Chei (0)

  • 나용호 (2)

  • Kim , Young-Ho (114)

  • Bae, Ki-Hwan (166)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기