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Capsaicin-Induced Apoptosis and Reduced Release of Reactive Oxygen Species in MBT-2 Murine Bladder Tumor Cells

Lee, Ji-Seon    (College of Pharmacy, Yeungnam University   ); Chang, Jong-Sun    (College of Pharmacy, Yeungnam University   ); Lee, Ji-Youl    (Department of Urology, College of Medicine, Catholic University of Korea   ); Kim, Jung-Ae    (College of Pharmacy, Yeungnam University  );
  • 초록

    Bladder cancer is a common cancer with high risk of recurrence and mortality. Intravesicle chemotherapy after trans-urethral resection is required to prevent tumor recurrence and progression. It has been known that antioxidants enhance the antitumor effect of bacillus Calmette-Guerin (BCG), the most effective intravesical bladder cancer treatment. Capsaicin, the major pungent ingredient in genus Capsicum, has recently been tried as an intravesical drug for overactive bladder and it has also been shown to induce apoptotic cell death in many cancer cells. In this study, we investigated the apoptosis-inducing effect and alterations in the cellular redox state of capsaicin in MBT-2 murine bladder tumor cells. Capsaicin induced apoptotic MBT-2 cell death in a time- and dose-dependent manner. The capsaicin-induced apoptosis was blocked by the pretreatment with Z-VAD-fmk, a broad-range caspase inhibitor, or Ac-DEVD-CHO, a caspase-3 inhibitor. In addition to the caspase-3 activation, capsaicin also induced cytochrome c release and decrease in Bcl-2 protein expression with no changes in the level of Bax. Furthermore, capsaicin at the concentration of inducing apoptosis also markedly reduced the level of reactive oxygen species and lipid peroxidation, implying that capsaicin may enhance the antitumor effect of BCG in bladder cancer treatment. These results further suggest that capsaicin may be a valuable intravesical chemotherapeutic agent for bladder cancers.


  • 주제어

    Capsaicin .   Bladder cancer .   Apoptosis .   Reactive oxygen species .   Bcl-2 .   Cytochrome c release .   Caspase-3.  

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  • 이 논문을 인용한 문헌 (5)

    1. Kwak, Chung-Shil ; Kim, Mee-Yeon ; Lee, Mee-Sook 2005. "Antioxidative Effect of Plant Food Mixtures in Rat Fed on High Fat-High Cholesterol Diet" 韓國營養學會誌 = The Korean journal of nutrition., 38(5): 352~363     
    2. 2007. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 30(1): 28~33     
    3. Kim, Sun Young ; Lee, You Jin ; Park, Eun Hye ; Yi, Ho Keun ; Jo, Dae Sun ; Kim, Jung Soo ; Hwang, Pyoung Han 2008. "Capsaicin induced apoptosis and the enhanced anticancer effect of anticancer drugs in cancer cells" Korean journal of pediatrics, 51(3): 307~314     
    4. 2008. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 31(5): 606~610     
    5. Kim, Hyo-Eun ; Jang, Min-Jeong ; Lim, Seung-Hyun ; Kim, Hyo-Rim ; Kim, Soon-Young ; Lee, Gun-Joo ; Kim, Jong-Sik 2010. "Analysis of p53-Dependency of Differentially Expressed Genes by Capsaicin in Human Colorectal Cancer Cell" 생명과학회지 = Journal of life science, 20(2): 213~218     

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