본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Decreased Pain Sensitivity of Capsaicin-Treated Rats Results from Decreased VR1 Expression

Lee, Soon-Youl    (Department of Genomic Engineering, Genetic Informatics Center, GRRC Hankyong National University   ); Hong, Young-Mi    (The Sensory Research Center, National Creative Research Initiatives, College of Pharmacy, Seoul National University   ); Oh, Uh-Taek    (The Sensory Research Center, National Creative Research Initiatives, College of Pharmacy, Seoul National University  );
  • 초록

    We investigated the neurotoxic effects of capsaicin (CAP) on pain sensitivity and on the expression of capsaicin receptor, the vanilloid receptor (VR1), in rats. High-dose application of CAP has been known to degenerate a large fraction of the sensory neurons. Although the neurotoxic effects of CAP are well documented, the effects of CAP on the vanilloid receptor (VR1) are not yet known. In this paper, we investigated the effects of high-dose application of CAP on the expression of VR1 in rats. Thermal and mechanical pain sensitivity was reduced when neonatal rats were treated with a high dose of CAP. This reduction of pain sensitivity was significantly decreased after initiating carrageenan-induced inflammation. The expression of VR1 in dorsal root ganglia (DRG) isolated from the CAP-treated rats was reduced compared to that from the vehicle-treated rats. Therefore, we can conclude that the neurotoxic effect of CAP is related to the decrease of VR1 expression.

  • 주제어

    Capsaicin .   Capsaicin ion channel .   Rat .   Dorsal root ganglion (DRG) .   Pain behav-ioral test .   Vanilloid receptor (VR 1) expression.  

  • 참고문헌 (25)

    1. Arvidsson, J. and Ygge, J., A quantitative study of the effects of neonatal capsaicin treatment and of subsequent peripheral nerve transection in the adult rat. Brain Res., 397, 130-136 (1986) 
    2. Gamse, R., Capsaicin and nociception in the rat and mouse. Possible role of substance P. Naunyn Schmiedebergs. Arch. Pharmacol., 320, 205-216 (1982) 
    3. Hammond, D. L. and Ruda, M. A., Developmental alterations in nociceptive threshold, immunoreactive calcitonin generelated peptide and substance P, and fluoride-resistant acid phosphatase in neonatally capsaicin-treated rats. J. Camp. Neurol.,312, 436-450 (1991) 
    4. Laszlo, U., Elizabath, A. C., Moh, P., Sadhana, P., and Naureen, C., In vivo pharmacology of SDZ 249-665, a novel, nonpungent capsaicin analogue. Pain, 89, 65-74 (2000) 
    5. Ren, K., Williams, G. M., Ruda, M. A., and Dubner, R., Inflammation and hyperalgesia in rats neonatally treated with capsaicin: effects on two classes of nociceptive neurons in the superficial dorsal horn. Pain, 59, 287-300 (1994) 
    6. Smart, D., Gunthorpe, M. J., Jerman, J. C., Nasir, S., Gray, J., Muir, A. I., Chambers, J. K., Randall, A. D., and Davis, J. B., The endogenous lipid anandamide is a full agonist at the human vanilloid receptor (hVR1). Br. J. Pharmacol., 129, 227-230 (2000) 
    7. Gao, X., Zhang, Y., and Wu, G., Effects of dopaminergic agents on carrageenan hyperalgesia in rats. Eur. J. Pharmacol., 406, 53-58 (2000) 
    8. Hammond, D. L. and Ruda, M. A., Developmental alterations in thermal nociceptive threshold and the distribution of immuno-reactive calcitonin gene-related peptide and substance P after neonatal administration of capsaicin in the rat. Neurasci. Lett., 97, 57-62 (1989) 
    9. Zygmunt, P. M., Peterson, J., Andersson, D. A., Chuang, H., Sorgard, M., Di Marzo, V., Julius, D., and Hogestatt, E. D., Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide. Nature, 400, 452-457 (1999) 
    10. Shin, J., Cho, H., Hwang, S. W., Jung, J., Shin, C. Y., Lee, S. Y., Kim, S. H., Lee, M. G., Choi, Y. H., Kim, J., Haber, N. A., Reichling, D. B., Khasar, S., Levine, J. D., and Oh, U., Bradykinin-12-lipoxygenase-VR1 signaling pathway for inflammatory hyperalgesia. Proc. Natl. Acad. Sci. U.S.A., 99, 10150-10155 (2002) 
    11. Caterina, M. J., Leffler, A., Malmberg, A. B., Martin, W. J., Trafton, J., Petersen-Zeitz, K. R., Koltzenburg, M., Basbaum, A. I., and Julius, D., Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science, 288, 306-313 (2000) 
    12. Jancso, G., Kiraly, E., and Jancso-Gabor, A., Pharmacologically induced selective degeneration of chemosensitive primary sensory neurones. Nature, 270, 741-743 (1977) 
    13. Hylden, J. L., Noguchi, K., and Ruda, M. A., Neonatal capsaicin treatment attenuates spinal Fos activation and dynorphin gene expression following peripheral tissue inflammation and hyperalgesia. J. Neurasci., 12, 1716-1725 (1992) 
    14. Kwak, J. Y., Jung, J. Y., Hwang, S. W., Lee, W. T., and Oh U., A capsaicin-receptor antagonist, capsazepine, reduces inflammation-induced hyperalgesic responses in the rat: evidence for an endogenous capsaicin-like substance. Neuroscience, 86, 619-626 (1998) 
    15. Gamse, R., Holzer, P., and Lembeck, F., Decrease of substance P in primary afferent neurones and impairment of neurogenic plasma extravasation by capsaicin. Br. J. Pharmacol., 68, 207-213 (1980) 
    16. Buck, S. H. and Burks, T. F., The neuropharmacology of capsaicin: review of some recent observations. Pharmacol. Rev., 38, 179-226 (1986) 
    17. Caterina, M. J., Schumacher, M. A., Tominaga, M., Rosen, T. A., Levine, J. D., and Julius, D., The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature, 389, 816-824 (1997) 
    18. Davis, J. B., Gray, J., Gunthorpe, M. J., Hatcher, J. P., Davey, P. T., Overend, P., Harries, M. H., Latcham, J., Clapham, C., Atkinson, K., Hughes, S. A., Rance, K., Grau, E., Harper, A. J., Pugh, P. L., Rogers, D. C., Bingham, S., Randall, A., and Sheardown, S. A., Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia. Nature, 405, 183-187 (2000) 
    19. Gamse, R., Petsche, U., Lembeck, F., and Jancso, G., Capsaicin applied to peripheral nerve inhibits axoplasmic transport of substance P and somatostatin. Brain Res., 239, 447-462 (1982) 
    20. McDougal, D. B. Jr, McDougal, S. H., and Johnson, E. M. Jr., Effect of capsaicin upon fluoride sensitive acid phosphatases in selected ganglia and spinal cord and upon neuronal size and number in dorsal root ganglion. Brain Res., 331, 63-70 (1985) 
    21. Oh, U., Hwang, S. W., and Kim, D., Capsaicin activates a nonselective cation channel in cultured neonatal rat dorsal root ganglion neurons. J. Neurosci., 16, 1659-1667 (1996) 
    22. Fitzgerald, M., Capsaicin and sensory $neurones-{\alpha}$ review. Pain, 15, 109-130 (1983) 
    23. Kwan, C. L., Hu, J. W., and Sessle, B. J., Neuroplastic effects of neonatal capsaicin on neurons in adult rat trigeminal nucleus principalis and subnucleus oralis. J. Neurophysiol., 75, 298-310 (1996) 
    24. Hwang, S. W., Cho, H., Kwak, J., Lee, S. Y., Kang, C. J., Jung, J., Cho, S., Min, K. H., Suh, Y. G., Kim, D., and Oh, U., Direct activation of capsaicin receptors by products of lipoxy-genases: endogenous capsaicin-like substances. Proc. Natl. Acad. Sci. U.S.A., 97, 6155-6160 (2000) 
    25. Holzer, P., Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons. Pharmacol. Rev., 43, 143-201 (1991) 
  • 이 논문을 인용한 문헌 (1)

    1. Um, Ki-Bum ; Lee, Soon-Youl 2011. "Role of Rab11 on Membrane Trafficking of Rat Vanilloid Receptor, TRPV1" 한국산학기술학회논문지 = Journal of the Korea Academia-Industrial cooperation Society, 12(7): 3096~3102     

 저자의 다른 논문

  • Lee, Soon-Youl (9)

    1. 2006 "알칼리성 단백질 분해 효소 생산 균주 Gelidibacter sp. HK-1의 분리 및 특성" 한국식품영양학회지 = The Korean journal of food and nutrition 19 (4): 496~501    
    2. 2006 "제주산 파인애플 유래 Bromelain관련 유전자 (BL1)를 이용반 형질전환 상추의 특성" 식물생명공학회지 = Korean journal of plant biotechnology 33 (1): 27~32    
    3. 2007 "단백질 분해효소를 생산하는 호염성 미생물 Vibrio의 분리 및 특성" 한국식품영양학회지 = The Korean journal of food and nutrition 20 (2): 108~113    
    4. 2009 "갯벌로부터 분리된 미생물에 의해 생산된 지질 분해 효소의 특성" 한국식품영양학회지 = The Korean journal of food and nutrition 22 (1): 14~19    
    5. 2011 "Functional Analysis of SGR4635-Induced Enhancement of Pigmented Antibiotic Production in Streptomyces lividans" The journal of microbiology 49 (5): 828~833    
    6. 2012 "Rat의 후근 신경절 세포에서의 Rab11-FIP3 단백질 발현 저해가 TRPV1 채널의 세포막으로의 이동에 미치는 영향" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 40 (3): 278~281    
    7. 2012 "Glutathione S-Transferase에 융합한 재조합 Hybrid Peptide Gaegurin-LL37의 대장균에서의 발현" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 40 (2): 92~97    
    8. 2014 "한천 분해효소를 생산하는 해양 미생물 SH-1의 분리 및 특성 분석" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 42 (4): 324~330    
  • Hong, Young-Mi (0)

  • Oh, Uh-Taek (11)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역



유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기