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The Korean journal of parasitology v.42 no.4 = no.132, 2004년, pp.169 - 174  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Organ-specific antigens of Clonorchis sinensis

Li, Shun-Yu    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Department of Parasitology, College of Medicine, Yanbian University   ); Chung, Byung-Suk    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center   ); Choi, Min-Ho    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center   ); Hong, Sung-Tae    (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center  );
  • 초록

    This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17 -kDa were main component of intestinal fluid and ES protein and commonly found in all organ-specific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.


  • 주제어

    Clonorchis sinensis .   organ-specific antigen .   immunoblot .   7-kDa antigen.  

  • 참고문헌 (18)

    1. Choi MH, Ryu JS, Lee M, et al. (2003b) Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda). Korean J Parasitol 41: 155-163     
    2. Chung YB, Yang HJ, Hong SJ, et al. (2003) Molecular cloning and immunolocalization of the 17 kDa myoglobin of Clonorchis sinensis. Parasitol Res 90: 365-368 
    3. Hong SJ, Kim TY, Song KY, Sohn WM, Kang SY (2001) Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection. Korean J Parasitol 39: 307-312. 
    4. Hong ST (2003) Clonorchis sinensis. In International Handbook of Foodborne Pathogens. pp 581-592. Marcel & Dekker, New York, USA 
    5. Lee M, Chung YB, Lee SK, et al. (2004) The identification of a Clonorchis sinensis gene encoding an antigenic egg protein. Parasitol Res (in press) 
    6. Rim HJ (1986) The current pathobiology and chemotherapy of clonorchiasis. Korean J Parasitol 24 (suppl): 1-141 
    7. Yong TS, Im K, Chung PR (1991) Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies. Korean J Parasitol 29: 293-310 
    8. Kim SI (1994) Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits. Korean J Parasitol 32: 35-42 
    9. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685 
    10. Chung YB, Lee M, Yang HJ, et al. (2002) Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis. Korean J Parasitol 40: 83-88     
    11. Choi MH, Park IC, Li S, Hong ST (2003a) Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA. Korean J Parasitol 41: 35-39 
    12. Kang SY, Ahn IY, Park CY, et al. (2001) Clonorchis sinensis: Molecular cloning and characterization of 28-kDa glutathione S-transferase. Exp Parasitol 97: 186-195 
    13. Kim SI (1998) A Clonorchis sinensis-specific antigen that detects active human clonorchiasis. Korean J Parasitol 36: 37-45 
    14. Sim S, Park GM, Yong TS (2003) Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens. Parasitol Res 91: 338-343 
    15. Ministry of Health and Welfare, Korea Association of Health Promotion (1997) Prevalence of intestinal parasitic infection in Korea. The sixth report, Seoul, pp 20-48 
    16. Hong SJ, Seong KY, Sohn WM, Song KY (2000) Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis. Mol Biochem Parasitol 108: 207-216 
    17. Hong ST, Kho WG, Lee M, Lee JS, Lee SH (1997) Immunoblot patterns of clonorchiasis. Korean J Parasitol 35: 87-93 
    18. Stitt AW, Fairweather I, Trudgett AG, Johnston CF (1992) Fasciola hepatica: localization and partial characterization of tubulin. Parasitol Res 78: 103-107 

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  • Chung, Byung-Suk (7)

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    6. 2003 "Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)" The Korean journal of parasitology 41 (3): 155~163    
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