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Journal of plant biotechnology v.6 no.3, 2004년, pp.189 - 192  

Direct Organogenesis in Geophila reniformis D. Don., an Important Medicinal Herb

Nisha, A.    (Food Microbiology Department, Central Food Technological Research Institute   ); Narasimhan, S.    (Department of Botany, University of Kerala, Thiruvananthapuram   ); Manjula, S.    (Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram   ); Nair, G.M.    (Tropical Botanic Garden & Research Institute, Palode, Thiruvananthapuram  );
  • 초록

    Adventitious multiple shoots were developed from leaf, petiole and internode explants of Geophila reniformis D. Don. on MS medium supplemented with various concentrations of $N^6$ -benzylaminopurine (BAP) or Kinetin (KIN) alone or in combination with indole-3-acetic acid (IAA). Leaf showed maximum organogenetic potential, followed by petiole and internode. Murashige and Skoog (MS) medium supplemented with 22.22 $\mu{M}$ BAP and 4.57 $\mu{M}$ IAA induced maximum shoot buds from leaf explants. Internodal segments showed low potential of direct organogenesis. The regenerated shoots rooted the best in presence of 10.75 - 13.44 $\mu{M}$ $\alpha$ -naphthalene acetic acid (NAA) along with 2.22 $\mu{M}$ BAP, and were successfully established in the field with a survival rate of 89.11%.


  • 주제어

    Direct organogenesis .   Geophila reniformis .   Rubiaceae.  

  • 참고문헌 (11)

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