본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

An Integrated Cell Processor for Single Embryo Manipulation

Park, Jung-Yul    (Microsystem Research Center, Korea Institute of Science and Technology   ); Jung, Seng-Hwan    (Digital Bio Technology   ); Kim, Young-Ho    (Microsystem Research Center, Korea Institute of Science and Technology   ); Kim, Byung-Kyu    (Microsystem Research Center, Korea Institute of Science and Technology   ); Lee, Seung-Ki    (School of Electrical, Electronic and Computer Engineering, Dankook University   ); Ju, Byeong-Kwon    (Microsystem Research Center, Korea Institute of Science and Technology  );
  • 초록

    In this paper, we present a novel integrated cell processor to handle individual embryos. Its functions are composed of transporting, isolation, orientation, and immobilization of cells. These functions are essential for biomanipulation of single cells, and have been typically carried out by a proficient operator. The purpose of this study is the automation of these functions for safe and effective cell manipulation using a MEMS based cell processor. This device is realized with a relatively simple design and fabrication process. Experimental results indicate that it can act as an efficient substitute for essential but very tiresome and repetitive manual work while contributing significantly to the improvement of speed and success rate of operation by facilitating cell manipulation. The cell viability test for the device is studied through the distribution of mitochondria in mice embryos and cultivation of cells for 86h.


  • 주제어

    biomanipulation .   cell processor .   dielectrophoresis .   polypyrrole.  

  • 참고문헌 (19)

    1. W. Lewis, B. Rosa, L. Peter, M. Elliot, S. Jim, and M. J. Thomas, Principles of development, Oxford University Press, 2nd edition, 2002 
    2. S. Masuda, M. Washizu, and T. Nanba, 'Novel methods of cell fusion and handling using fluid integrating circuit,' Seventh International Conference on Electrostatic Phenomena (Electrosatitcs 87), Oxford, U.K., pp.69-74, 1987 
    3. M. P. Hughes, Nanoelectromechanics in Enginnering and Biology, CRC Press, 2003 
    4. T. Muller, G. Gradl, S. Howitz, S. Shirley, T. Schnelle, G. Fuhr, 'A 3-D microelectde system for handling and caging single cells and particles,' Biosensor & Bioelectronics, vol. 14, 1999, pp. 247-256 
    5. S. A. Kaur and H. S. Virk, 'Conduction of bacteria and blood cells through ply carbonate sieves,' Indian J. Pure Appl. Phys., vol.33, pp.350-352, 1995 
    6. L. Westerlaken, F. Helmerhorst, J. Hermans, and N. Naaktgeboren, 'Intracytoplasmic sperm injection: position of the polar body affects pregnancy rate,' Human Reproduction, vol.14, no.10,1999, pp.2565-2569 
    7. B. Kim, D-H. Kim, J-Y. Park, Y-H. Kim, S-J. Kwon, H. Kang, and S. Jung, 'Autonomous Biomanipulation Factory for Manipulating Individual Embryo Cells,' Invited Paper, 2003 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2003), Las Vegas, USA, October 27-November 1, 2003. (Workshop: Microrobotics for Biomanipulation) 
    8. J. E. Molloy and M. J. Padgett, 'Lights, action: optical tweezers,' Contemporary Physics, vol. 43, pp.241-258, 2002 
    9. R. Pethig and G. Markx, 'Applications of dielectrophoresis in biotechnology,' Trends in Biotechnolgoy, vol.15, 1997, pp. 426-432 
    10. Y. Sun and B.J. Nelson, 'Biological cell injection using an autonomous microrobotic system,' The International Journal of Robotics Research (IJRR), vol.21, pp.861-868, 2002 
    11. E. Jager, C. Immerstrand, K. Peterson, K. Magnusson, I. Lundstrom, and O. Inganas, 'The Cell Clinic: Closable Microvials for Single Cell Studies,' Biomedical Microdevices, vol. 4, 2002, pp.177-187 
    12. R. H. Carlson, C. Grabel, S. Chan, and R. H. Austin, 'Activation and sorting of human white blood cells,' Biomedical Microdevices, vol.1, pp.39-47, 1998 
    13. T. Nakayama, H. Fujiwara, K. Tastumi, K. Fujita, T. Higuchi, and T. Mori, 'A new assisted hatching technique using a piezo-micromanipulator,' Fertility and Sterility, vol.69, no.4, 1998 
    14. M. P. Sheetz, in Laser Tweezers in Cell Biology, vol. 55 of Methods in Cell Biology, Academic Press, New York, 1997 
    15. I. Glasgow, H. Zeringue, D. Beebe, S. Choi, J. Lyman, N. Chan, and M. Wheeler, 'Handling Individual mammalian embryos using microfluidics,' IEEE Transactions on Biomedical Engineering, vol. 48, no. 5, May 2001, pp. 570-577 
    16. J. Ryu, S. Jung, B. Kim, S-K.Lee, and J-O.Park, 'Thickness characteristics and improved surface adhesion of a polypyrrole actuator by analysis of polymerization process,' Polymer-Korea, submitted     
    17. K. K. Tan and S. C. Ng, 'Computer-controlled piezo micromanipulation system for biomedical applications,' Engineering Science and Education Journal, pp.249-256, 2001 
    18. K. Yanagida, H. Katayose, H. Yazawa, Y. Kimura, K. Konnai, and A. Sato, 'The usefulness of a piezomicromanipulator in intracytoplasmic sperm injection in humans,' Human Reproduction, Vol.14, No.2, pp.448-453, 1998 
    19. E. Smela, O. Inganas, and I. Lundstrom, 'Differential Adhesion Method for Microstructure Release: An Alternative to the Sacrificial Layer,' Transducers '95, Stockholom, Sweden, 1995, pp. 218-21 

 저자의 다른 논문

  • 이승기 (22)

    1. 1997 "The Optimum Condition of Anisotropic Bulk(10) Si Etching with KOH for High Selectivity and Low Surface Roughness" Journal of electrical engineering and information science 2 (5): 108~113    
    2. 1998 "Bias 스프링을 이용한 형상기억합금 액츄에이터의 설계 방법" 센서학회지 = Journal of the Korean Sensors Society 7 (6): 437~445    
    3. 1998 "합성조건의 변화에 따른 액츄에이터 재료로서의 전도성 고분자의 기계적 특성" 센서학회지 = Journal of the Korean Sensors Society 7 (6): 446~451    
    4. 1998 "전도성 고분자를 이용한 마이크로 액츄에이터" 전기전자재료 = Bulletin of the Korean institute of electrical and electronic material engineers 11 (4): 339~349    
    5. 1999 "PSG 희생층 식각시 Al층을 보호하기 위한 새로운 HF/$NH_4F$/Glycerine 혼합 식각액" 센서학회지 = Journal of the Korean Sensors Society 8 (5): 414~420    
    6. 2000 "표면 미세가공에서 Al 전극 및 Al 미세 구조물 제작을 위한 습식 식각 공정" 센서학회지 = Journal of the Korean Sensors Society 9 (3): 224~232    
    7. 2000 "전도성 고분자를 이용한 마이크로 액추에이터 제작" 전기학회논문지. The transactions of the Korean Institute of Electrical Engineers. C/ C, 전기물성·응용부문 49 (12): 698~704    
    8. 2001 "$NH_4OH-H_2O_2-H_2O$ 혼합액을 이용한 GaAS의 습식식각 특성 연구 및 이를 이용한 부유된 사각형 단면을 가지는 GaAs 미세구조물의 제작 방법" 센서학회지 = Journal of the Korean Sensors Society 10 (5): 304~313    
    9. 2001 "기능성 재료의 액추에이터 응용" 전기전자재료 = Bulletin of the Korean institute of electrical and electronic material engineers 14 (2): 19~28    
    10. 2002 "백금의 무 전해 도금에 의한 이온성 고분자-금속 복합물 액추에이터의 제작 공정 및 특성 측정" 전기학회논문지. The transactions of the Korean Institute of Electrical Engineers. C/ C, 전기물성·응용부문 51 (9): 455~463    
  • 주병권 (130)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기