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An Integrated Cell Processor for Single Embryo Manipulation

Park, Jung-Yul    (Microsystem Research Center, Korea Institute of Science and Technology   ); Jung, Seng-Hwan    (Digital Bio Technology   ); Kim, Young-Ho    (Microsystem Research Center, Korea Institute of Science and Technology   ); Kim, Byung-Kyu    (Microsystem Research Center, Korea Institute of Science and Technology   ); Lee, Seung-Ki    (School of Electrical, Electronic and Computer Engineering, Dankook University   ); Ju, Byeong-Kwon    (Microsystem Research Center, Korea Institute of Science and Technology  );
  • 초록

    In this paper, we present a novel integrated cell processor to handle individual embryos. Its functions are composed of transporting, isolation, orientation, and immobilization of cells. These functions are essential for biomanipulation of single cells, and have been typically carried out by a proficient operator. The purpose of this study is the automation of these functions for safe and effective cell manipulation using a MEMS based cell processor. This device is realized with a relatively simple design and fabrication process. Experimental results indicate that it can act as an efficient substitute for essential but very tiresome and repetitive manual work while contributing significantly to the improvement of speed and success rate of operation by facilitating cell manipulation. The cell viability test for the device is studied through the distribution of mitochondria in mice embryos and cultivation of cells for 86h.

  • 주제어

    biomanipulation .   cell processor .   dielectrophoresis .   polypyrrole.  

  • 참고문헌 (19)

    1. W. Lewis, B. Rosa, L. Peter, M. Elliot, S. Jim, and M. J. Thomas, Principles of development, Oxford University Press, 2nd edition, 2002 
    2. S. Masuda, M. Washizu, and T. Nanba, 'Novel methods of cell fusion and handling using fluid integrating circuit,' Seventh International Conference on Electrostatic Phenomena (Electrosatitcs 87), Oxford, U.K., pp.69-74, 1987 
    3. M. P. Hughes, Nanoelectromechanics in Enginnering and Biology, CRC Press, 2003 
    4. T. Muller, G. Gradl, S. Howitz, S. Shirley, T. Schnelle, G. Fuhr, 'A 3-D microelectde system for handling and caging single cells and particles,' Biosensor & Bioelectronics, vol. 14, 1999, pp. 247-256 
    5. S. A. Kaur and H. S. Virk, 'Conduction of bacteria and blood cells through ply carbonate sieves,' Indian J. Pure Appl. Phys., vol.33, pp.350-352, 1995 
    6. L. Westerlaken, F. Helmerhorst, J. Hermans, and N. Naaktgeboren, 'Intracytoplasmic sperm injection: position of the polar body affects pregnancy rate,' Human Reproduction, vol.14, no.10,1999, pp.2565-2569 
    7. B. Kim, D-H. Kim, J-Y. Park, Y-H. Kim, S-J. Kwon, H. Kang, and S. Jung, 'Autonomous Biomanipulation Factory for Manipulating Individual Embryo Cells,' Invited Paper, 2003 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2003), Las Vegas, USA, October 27-November 1, 2003. (Workshop: Microrobotics for Biomanipulation) 
    8. J. E. Molloy and M. J. Padgett, 'Lights, action: optical tweezers,' Contemporary Physics, vol. 43, pp.241-258, 2002 
    9. R. Pethig and G. Markx, 'Applications of dielectrophoresis in biotechnology,' Trends in Biotechnolgoy, vol.15, 1997, pp. 426-432 
    10. Y. Sun and B.J. Nelson, 'Biological cell injection using an autonomous microrobotic system,' The International Journal of Robotics Research (IJRR), vol.21, pp.861-868, 2002 
    11. E. Jager, C. Immerstrand, K. Peterson, K. Magnusson, I. Lundstrom, and O. Inganas, 'The Cell Clinic: Closable Microvials for Single Cell Studies,' Biomedical Microdevices, vol. 4, 2002, pp.177-187 
    12. R. H. Carlson, C. Grabel, S. Chan, and R. H. Austin, 'Activation and sorting of human white blood cells,' Biomedical Microdevices, vol.1, pp.39-47, 1998 
    13. T. Nakayama, H. Fujiwara, K. Tastumi, K. Fujita, T. Higuchi, and T. Mori, 'A new assisted hatching technique using a piezo-micromanipulator,' Fertility and Sterility, vol.69, no.4, 1998 
    14. M. P. Sheetz, in Laser Tweezers in Cell Biology, vol. 55 of Methods in Cell Biology, Academic Press, New York, 1997 
    15. I. Glasgow, H. Zeringue, D. Beebe, S. Choi, J. Lyman, N. Chan, and M. Wheeler, 'Handling Individual mammalian embryos using microfluidics,' IEEE Transactions on Biomedical Engineering, vol. 48, no. 5, May 2001, pp. 570-577 
    16. J. Ryu, S. Jung, B. Kim, S-K.Lee, and J-O.Park, 'Thickness characteristics and improved surface adhesion of a polypyrrole actuator by analysis of polymerization process,' Polymer-Korea, submitted     
    17. K. K. Tan and S. C. Ng, 'Computer-controlled piezo micromanipulation system for biomedical applications,' Engineering Science and Education Journal, pp.249-256, 2001 
    18. K. Yanagida, H. Katayose, H. Yazawa, Y. Kimura, K. Konnai, and A. Sato, 'The usefulness of a piezomicromanipulator in intracytoplasmic sperm injection in humans,' Human Reproduction, Vol.14, No.2, pp.448-453, 1998 
    19. E. Smela, O. Inganas, and I. Lundstrom, 'Differential Adhesion Method for Microstructure Release: An Alternative to the Sacrificial Layer,' Transducers '95, Stockholom, Sweden, 1995, pp. 218-21 

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