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Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Lithospermic Acid B in Rat Serum

Kim, Hui-Hyun    (College of Pharmacy and Phytofermentation Research Center, Wonkwang University   ); Ji, Hye-Young    (College of Pharmacy and Phytofermentation Research Center, Wonkwang University   ); Lee, Hye-Won    (College of Pharmacy and Phytofermentation Research Center, Wonkwang University   ); Kim, Youn-Chul    (College of Pharmacy and Phytofermentation Research Center, Wonkwang University   ); Sohn, Dong-Hwan    (College of Pharmacy and Phytofermentation Research Center, Wonkwang University   ); Lee , Hye-Suk    (College of Pharmacy and Phytofermentation Research Center, Wonkwang University  );
  • 초록

    A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/ MS) method for the determination of lithospermic acid B (LSB) in rat serum was developed. LSB and internal standard, 7-hydroxy-3-phenyl-chromen-4-one (HPC) were extracted from rat serum with methyl-tert-butyl ether at acidic pH and analyzed on a Luna $C_8$ column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 6.5) (50:50, v/v). The analytes were detected using a negative electrospray ionization tandem mass spectrometry in the multiple- reaction-monitoring mode. The standard curve was linear $(r^2 = 0.997)$ over the concentration range of 10.0-500 ng/mL. The coefficient of variation and relative error for intra- and interassay at three QC levels were 1.1~6.2% and -10.3~-2.7%, respectively. The recovery of LSB from serum sample ranged from 73.2 to 79.5%, with that of HPC (internal standard) being 75.1 %. The lower limit of quantification for LSB was 10 ng/mL using 50 ${\mu}L$ of serum sample.


  • 주제어

    Lithospermic acid B .   Rat serum .   LC-MS/MS.  

  • 참고문헌 (9)

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 저자의 다른 논문

  • Kim, Hui-Hyun (1)

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  • Ji, Hye-Young (5)

  • Lee, Hye-Won (1)

  • Kim, Youn-Chul (66)

  • Lee, Hye-Suk (16)

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