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Apoptosis Inducing Effects of 6-Methoxydihydrosanguinarine in HT29 Colon Carcinoma Cells

Lee, Yong-Jin    (College of Pharmacy and MeRRI, Wonkwang University   ); Yin, Hu-Quan    (College of Pharmacy and MeRRI, Wonkwang University   ); Kim, Young-Ho    (College of Pharmacy, Chungnam National University   ); Li, Guang-Yong    (College of Pharmacy and MeRRI, Wonkwang University   ); Lee , Byung-Hoon    (College of Pharmacy and MeRRI, Wonkwang University  );
  • 초록

    6-Methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 ${\mu}M$ on causing apoptotic cell death in HT29 colon carcinoma cells $(IC_{50} = 5.0{\pm}0.2 {\mu}M)$ . Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.


  • 주제어

    6-Methoxydihydrosanguinarine .   Hylomecon hylomeconoides .   Apoptosis .   HT29 cells.  

  • 참고문헌 (14)

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