본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Rolipram, a Phosphodiesterase 4 Inhibitor, Stimulates Osteoclast Formation by Inducing TRANCE Expression in Mouse Calvarial Cells

Cho, Eun-Sook    (College of Pharmacy, Sookmyung Women's University   ); Yu, Ja-Heon    (College of Pharmacy, Sookmyung Women's University   ); Kim, Mi-Sun    (College of Pharmacy, Sookmyung Women's University   ); Yim, Mi-Jung    (College of Pharmacy, Sookmyung Women's University  );
  • 초록

    Phosphodiesterase (PDE) 4 is an enzyme that degrades intracellular cAMP. In the present study, the effect of rolipram, a specific phosphodiesterase (PDE) 4 inhibitor, on osteoclast formation was investigated. Rolipram induced osteoclast formation in cocultures of mouse bone marrow cells and calvarial osteoblasts. This activity was not observed in the absence of calvarial osteoblasts, suggesting that calvarial osteoblasts are likely target cells of rolipram. Osteoclast formation by rolipram was completely blocked by the addition of osteoprotegerin (OPG), a soluble decoy receptor for the osteoclast differentiation factor, TNF-related activation-induced cytokine (TRANCE, identical to RANKL, ODF, and OPGL). Northern blot analysis revealed the effect of rolipram to be associated with the increased expression of TRANCE mRNA in mouse calvarial osteoblasts. Collectively, these data indicate that PDE4 inhibitor up-regulates the TRANCE mRNA expression in osteoblasts, which in turn controls osteoclast formation.


  • 주제어

    Osteoblast .   Osteoclast .   cAMP .   Phosphodiesterase 4 inhibitor .   TRANCE.  

  • 참고문헌 (18)

    1. Conti, M. and Jin, S. L., The molecular biology of cyclic nucleotide phosphodiesterases. Prog. Nucleic. Acid Res. Mol. Biol., 63,1-38 (1999) 
    2. Kondo, H., Guo, J., and Bringhurst, F. R., Cyclic adenosine monophosphate/protein kinase A mediates parathyroid hormone/parathyroid hormone-related protein receptor regulation of osteoclastogenesis and expression of RANKL and osteoprotegerin mRNAs by marrow stromal cells. J. Bone Miner. Res., 17, 1667-1679 (2002) 
    3. Tsutsumimoto, T., Wakabayashi, S., Kinoshita, T., Horiuchi, H., and Takaoka, K., A phosphodiesterase inhibitor, pentoxifylline, enhances the bone morphogenetic protein-4 (BMP-4)dependent differentiation of osteoprogenitor cells. Bone, 31, 396-401 (2002) 
    4. Houslay, M. D., Sullivan, M., and Bolger, G. B., The multienzyme PDE4 cyclic adenosine monophosphate-specific phosphodiesterase family: intracellular targeting, regulation, and selective inhibition by compounds exerting anti-inflammatory and antidepressant actions. Adv. Pharmacol., 44, 225-342 (1998) 
    5. Waki, Y., Horita, T., Miyamoto, K., Ohya, K., and Kasugai, S., Effects of XT-44, a phosphodiesterase 4 inhibitor, in osteoblastgenesis and osteoclastgenesis in culture and its therapeutic effects in rat osteopenia models. Jpn. J Pharmacol., 79, 477-483 (1999) 
    6. Houslay, M. D. and Adams, D. R., PDE4 cAMP phosphodie sterases: modular enzymes that orchestrate signalling crosstalk, desensitization and compartmentalization. Biochem. J, 370, 1-18 (2003) 
    7. Takahashi, N., Udagawa, N., and Suda, T. A., New member of tumor necrosis factor ligand family, ODF/OPGL/TRANCE/ RANKL, regulates osteoclast differentiation and function. Biochem .Biophys. Res.Commun., 256, 449-455 (1999) 
    8. Suda, T., Jimi, E., Nakamura, I., and Takahashi, N., Role of 1 alpha, 25-dihydroxyvitamin D3 in osteoclast differentiation and function. Methods Enzymol., 282, 223-235 (1997) 
    9. Kinoshita, T., Kobayashi, S., Ebara, S., Yoshimura, Y., Horiuchi, H., Tsutsumimoto, T., Wakabayashi, S., and Takaoka, K., Phosphodiesterase inhibitors, pentoxifylline and rolipram, increase bone mass mainly by promoting bone formation in normal mice. Bone, 27, 811-817 (2000) 
    10. Wong, B. R., Rho, J., Arron, J., Robinson, E., Orlinick, J., Chao, M., Kalachikov, S., Cayani, E., Bartlett 3rd, F. S., Frankel, W. N., Lee, S. Y., and Choi, Y., TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun Nterminal kinase in T cells. J. Biol. Chem., 272, 25190-25194 (1997) 
    11. Miyamoto, K., Waki, Y., Horita, T., Kasugai, S., and Ohya, K., Reduction of bone loss by denbufylline, an inhibitor of phosphodiesterase 4. Biochem. Pharmacol., 54, 613-617 (1997) 
    12. Fu, Q., Jilka R. L., Manolagas, S. C., and O'Brien, C. A., Parathyroid hormone stimulates receptor activator of NF kappa B ligand and inhibits osteoprotegerin expression via protein kinase A activation of cAMP-response elementbinding protein. J. Biol. Chem., 277, 48868-48875 (2002) 
    13. Suda, T., Takahashi, N., Udagawa, N., Jimi, E, Gillespie, M. T., and Martin, T. J., Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr. Rev., 20, 345-357 (1999) 
    14. Tam, C. S., Heersche, J. N., Murray, T. M., and Parsons, J. A., Parathyroid hormone stimulates the bone apposition rate independently of its resorptive action: differential effects of intermittent and continuous administration. Endocrinology, 110, 506-512 (1982) 
    15. Vaananen, H. K., Zhao, H., Mulari, M., and Halleen, J. M., The cell biology of osteoclast function. J. Cell Sci., 113, 377-381 (2000) 
    16. Essayan, D. M., Cyclic nucleotide phosphodiesterases. J. Allergy CIin. lmmunol., 108, 671-680 (2001) 
    17. Wakabayashi, S., Tsutsumimoto, T., Kawasaki, S., Kinoshita, T., Horiuchi, H., and Takaoka, K., Involvement of phosphodiesterase isozymes in osteoblastic differentiation. J Bone Miner. Res., 17, 249-56 (2002) 
    18. Takahashi, N., Akatsu, T., Udagawa, N., Sasaki, T., Yamaguchi, A., Moseley, J. M., Martin, T. J., and Suda, T., Osteoblastic cells are involved in osteoclast formation. Endocrinology, 123, 2600-2602 (1988) 
  • 이 논문을 인용한 문헌 (3)

    1. Lee, Hyo-Keun ; Hwang, Gwi-Seo 2010. "Effects of Securinega suffructicosa(SS) Extract on Osteoclastogenesis and Gene Expression in RANKL-induced RAW Cell" 대한예방한의학회지 = Journal of Society of Preventive Korean Medicine, 14(3): 13~26     
    2. Song, Young-Hun ; Yoo, Jeong-Eun ; Lim, Hyun-Jung ; Yoo, Dong-Youl 2010. "Effects of Clematidis Radix Extract on Osteoclastogenesis and Gene Expression in RANKL-induced RAW 264.7 Cell" 大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, 23(3): 78~90     
    3. Kim, Joon-Yeon ; Hwang, Gwi-Seo 2010. "The Effects of Cuscuta japonica Chois on Gene Expression in RANKL-induced RAW 264.7 Cell" 대한예방한의학회지 = Journal of Society of Preventive Korean Medicine, 14(2): 77~89     

 저자의 다른 논문

  • Yim, Mi-Jung (14)

    1. 2003 "박테리오파아지 표면 발현 시스템을 이용한 Maackia amurensis Hemagglutinin (MAH)의 기능적 발현" 약학회지 = Yakhak hoeji 47 (3): 176~179    
    2. 2003 "IL-l/Toll-like Receptor Superfamily 신호전달에 관여하는 TRAF6 결합 Motif의 규명" 약학회지 = Yakhak hoeji 47 (3): 180~183    
    3. 2004 "Phosphodiesterase 저해제 Pentoxifylline이 파골세포 분화에 미치는 영향" 약학회지 = Yakhak hoeji 48 (3): 197~201    
    4. 2005 "조골세포내 cAMP 농도 변화가 파골세포 형성에 미치는 영향" 약학회지 = Yakhak hoeji 49 (1): 109~113    
    5. 2007 "Rolipram, a Phosphodiesterase 4 Inhibitor, Suppresses PGE$_2$- Induced Osteoclast Formation by Lowering Osteoclast Progenitor Cell Viability" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 30 (4): 486~492    
    6. 2007 "PDE 저해제에 의한 $PGE_2$의 파골세포 분화 유도 증강효과" 약학회지 = Yakhak hoeji 51 (4): 235~238    
    7. 2008 "LPS의 파골세포 분화 유도에 미치는 PDE4 저해제의 증강효과" 약학회지 = Yakhak hoeji 52 (1): 43~47    
    8. 2009 "Dexamethasone에 의한 RANKL 유도성 파골세포 분화 촉진 효과" 약학회지 = Yakhak hoeji 53 (2): 78~82    
    9. 2009 "골손실을 조절하는 활성산소종의 역할 규명" 약학회지 = Yakhak hoeji 53 (4): 179~183    
    10. 2010 "조골세포내 PDE4에 의한 PTH 신호의 음성적 조절" 약학회지 = Yakhak hoeji 54 (5): 410~415    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기