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Random Amplified Polymorphic DNA Analysis of Genetic Relationships Among Acanthopanax Species

Park, Sang-Yong    (College of Pharmacy, Kyung Hee University   ); Yook, Chang-Soo    (College of Pharmacy, Kyung Hee University   ); Nohara, Toshihiro    (Faculty of Pharmaceutical Sciences, Kumamoto University   ); Mizutani, Takayuki    (School of Agriculture, Kyushu Tokai University   ); Tanaka , Takayuki    (School of Agriculture, Kyushu Tokai University  );
  • 초록

    Random amplified polymorphic DNA (RAPD) analysis was used to determine the genetic relationships among seventeen species of the Acanthopanax species. The DNA isolated from the leaves of the samples was used as template in polymerase chain reaction (PCR) with twenty random decamer primers in order to distinguish plant subspecies at the level of their genomes. The RAPD patterns were compared by calculating pairwise distances using Dice similarity index, and produced to the genetic similarity dendrogram by unweighted pair-group method arithmetic averaged (UPGMA) analysis, showing three groups; a major cluster(twelve species), minor cluster (4 species) and single-clustering species. The results of RAPD were compatible with the morphological classification, as well as the chemotaxonomic classification of the Acanthopanax species. The Acanthopanax species containing 3,4-seco-lupane type triterpene compounds in their leaves corresponded to the major cluster, another species having oleanane or normal lupane type constituents to minor clusters, and one species not containing triterpenoidal compound to single-cluster.


  • 주제어

    Acanthopanax species .   RAPD .   UPGMA .   Genetic similarity .   Morphological classification .   Chemotaxonomy.  

  • 참고문헌 (20)

    1. Chang, S. Y., Yook, C. S., and Nohara, T., Two new lupans triterpene glycosides from leaves of Acanthopanax koreanum. Chem. Pharm.Bull.,46,163-165 (1998) 
    2. Kitajima, J. Takamori, Y. and Tanaka, Y., Studies on the constituents of Acanthopanax sciadophylloides Fr. et Sav. leaves. Yakukaku Zasshi, 109, 188-191 (1989) 
    3. Namba, T., Encyclopedia of Wakan-Yaku with color pictures. Hiokusa, Osaka, (1994) 
    4. Park, S. Y., Chang, S. Y., Yook, C. S., and Nohara, T., New 3,4seco- Iupane-type triterpene glycosides from Acanthopanax senticosus forma inermis. J. Nat. Prod., 63, 1630-1633 (2000) 
    5. Yook, C. S., Kim, I. L., Hahn, D. R., Nohara, T., and Chang, S. Y., A lupane-triterpene glycoside from leaves of two Acanthopanax. Phytochemistry, 49, 839-843 (1998) 
    6. Hosokawa, K., Minami, M., Kawahara, K., Nakamura, I., and Shibata, T., Discrimination among tree species of medical Scutellaria plants using RAPD markers. Planta Med., 66, 270-272 (2000) 
    7. Shaw, P. C. and But, P. P. H., Authentication of Panax species and their adulterants by random-primed polymerase chain reaction. Planta Med., 61, 466-469 (1995) 
    8. Oh, H. J., Chang, S. Y., Yook, C. S., Yang, K. S., Park, S.Y., and Nohara, T., Two 3,4-seco-lupane triterpenes from leaves of Acanthopanax divaricatus var. albeofeuctus. Chem. Pharm. Bull., 48, 879-881 (2000) 
    9. Paul, M. T., Tai, K. V., Kerry, K. S., and Raymond, J. S., Random amplified polymorphic DNA analysis of the phenetic relationships among world-wide accessions of Hydrilla verticillata. Aquat. Bot., 59, 217-223 (1997) 
    10. Dettori, M. T. and Palobi, M. A., Identification of Feijoa sellowiana Berg accessions by RAPD markers. Sci. Hortic., 86, 279-290 (2000) 
    11. Sawada, H., Miyakoshi, M., Isoda, S., Ida, Y., and Shoji, J., Oleanane type triterpene glycosyl esters from leaves of Acnathopanax sieboldianus. Phytochemistry, 34, 1117-1121 (1993) 
    12. Ohwi, J., Flora of Japan (rev. ed). Shibundo, Tokyo, (1972) 
    13. Yook, C. S., Illustrated Book of Medicinal plants. Kyung-Won, Seoul, (1997) 
    14. Shao, C. J., Kasei, R., Xu, J. D., and Tanaka, O., Saponin from leaves of Acanthopanax senticisus Harms., Ciwujia: Structures of ciwujianoside B, $C_1$, $C_2$, $C_3$, $C_4$, $D_1$, $D_2$, and E. Chem. Pharm. Bull., 36, 601-608 (1988) 
    15. Shao, C. J., Kasei, R., Xu, J. D., Tanaka, O., Saponin from leaves of Acanthopanax senticisus Harms., Ciwujia. II. Structures of ciwujianoside $A_1$, $A_2$, $A_3$, $A_4$, and $D_3$, Chem. Pharm. Bull., 37, 42-45 (1989) 
    16. Chang, S. Y., Yook, C. S., and Nohara, T., Lupane-triterpene glycosides from leaves of Acanthopanax koreanum. Phytochemistry, 50, 1369-1374 (1999) 
    17. Shirasuna, K., Miyakoshi, M., Mimoto, S., Isoda, S., Satoh, Y., Hirai, Y., Ida, Y., and Shoji, J., Lupane triterpene glycosyl esters from leaves of Acanthopanax divaricatus. Phytochemistry, 45, 579-584 (1997) 
    18. Kasei, R., Matsumoto, K., Taniyasu, S., Tanaka, O., Kim, J. H., and Hahn, D. R., 3,4-Seco-lupane type triterpene glycosyl esters from Korean medicinal plant, Acanthopanax chiisanensis. Chem. Pharm. Bull., 34, 3284-3289 (1986) 
    19. Miyakoshi, M., Shirasuna, K., Hirai, Y., Shingu, K., lsoda, S., Shoji, J., Ida, Y., and Shimizu, T., Triterpenoid saponins of Acanthopanaxniponicus leaves. J. Nat. Prod., 62, 445-448 (1999) 
    20. Sneath, P. H. A. and Sokal, R. R., Numerical taxonomy: The principals and practice of numerical classification. Freeman, San Francisco, (1973) 

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