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Journal of plant biology = 식물학회지 v.47 no.4, 2004년, pp.330 - 337   피인용횟수: 1
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Defects in a Proteolytic Step of Light-Harvesting Complex II in an Arabidopsis Stay-Green Mutant, ore10, during Dark-Induced Leaf Senescence

Oh, Min-Hyuk    (Department of Molecular Biology, Pusan National University   ); Kim, Jin-Hong    (Department of Molecular Biology, Pusan National University   ); Moon, Yong-Hwan    (Department of Molecular Biology, Pusan National University   ); Lee, Choon-Hwan    (Department of Molecular Biology, Pusan National University  );
  • 초록

    During dark-induced leaf senescence (DIS), the non-functional stay-green mutant ore10 showed delayed chlorophyll (Chl) degradation and increased stability in its light-harvesting complex II (LHCII). These phenomena were closely related to the formation of aggregates that mainly consisted of terminal-truncated LHCII (Oh et al., 2003). The ore10 mutant apparently lacks the protease needed to degrade the truncated LHCII. In wild-type (WT) plants, protease was found in the thylakoid fraction, but not the soluble fraction. A similar experiment using dansylated LHCII revealed that the protease degraded both WT and ore10 LHCII, indicating that its stability in ore10 perhaps did not result from a defect in the LHCII polypeptides themselves. Although protease activity was not present in non-senesced WT leaves, it was induced during DIS. It also was possible to diminish the high level of protease present in the thylakoids through high-salt washing, suggesting that this enzyme is extrinsically bound to the outer surface of the stroma-exposed thylakoid regions.


  • 주제어

    Arabidopsis thaliana .   leaf senescence .   light-harvesting complex II .   proteolysis .   stay-green mutant.  

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