본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biology = 식물학회지 v.47 no.4, 2004년, pp.289 - 299   피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Selection and Characterization of Transposon Tagging Mutants of Synechocystis sp. PCC 6803 Sensitive to High-Light and Oxidative Stresses

Song, Eun-Kyeong    (Department of Molecular Biology, Pusan National University   ); Zulfugarov, Ismayil S.    (Department of Molecular Biology, Pusan National University   ); Kim, Jin-Hong    (Department of Molecular Biology, Pusan National University   ); Kim, Eun-Ha    (Department of Molecular Biology, Pusan National University   ); Lee, Woo-Sung    (Department of Biological Science, Sungkyunkwan University   ); Lee, Choon-Hwan    (Department of Molecular Biology, Pusan National University  );
  • 초록

    We compared several analytical tools to identify which were most applicable for the selection and characterization of specific transposon-tagged mutant strains of Synechocystis sp. PCC 6803 that are sensitive to high light and oxidative stresses. Our primary parameter was the maximum photochemical efficiency of dark-adapted cells, a very sensitive factor that can be determined in a non-destructive manner. Using this as a tool for primary selection, we identified five mutant strains with different sensitivities to photoinhibition and photooxidation. For further characterization, we obtained data describing the absorption spectra for pigment contents, the 77 K fluorescence spectra, non-photochemical quenching (as a down-regulation process), and the photosynthetic electron transfer rate. Based on these results, we were able to design a strategy for selecting mutants with specific phenotypes. Here, we also discuss the strengths and weaknesses of each selection and characterization tool.

  • 주제어

    chlorophyll fluorescence .   cyanobacterium .   high light .   oxidative stree .   photoinhibition.  

  • 참고문헌 (41)

    1. Ashby NK, Mullineaux CW (1999) The role of ApcD and ApcF in energy transfer from phycobilisomes to PS I and PS II in a cyanobacterium. Photosynth Res 61: 169 
    2. Campbell D, Hurry V, Clarke AK, Gustaffron P, $\"O$quist G (1998) Chlorophyll fluorescence analysis of cyanobacte-rial photosynthesis and acclimation. Microbiol Mol Biol Rev 62: 667-683 
    3. Demmig-Adams B, Adams WW III (1992) Photoprotection and other responses of plants to high light stress. Annu Rev Plant Physiol Plant Mol Biol 43: 599-626 
    4. Eu Y-J, Lee M-H, Chang H-S, Rhew TH, Lee HY, Lee C-H (1998) Chlorophyll fluorescence assay for kanamycin resistance screening in transgenic plants. Plant Cell Rep 17:189-194 
    5. Fujita Y, Murakami A, Ohki K (1987) Regulation of photosystem composition in the cyanobacterial photosynthetic system: The regulation occurs in response to the redox state of the electron pool located between the two photosystems. Plant Cell Physiol 28: 283-292 
    6. Katayama M, Tsinoremas NF, Kondo T, Golden SS (1999) cpmA, a gene involved in an output pathway of the cyanobacterial circadian system. J Bacteriol 181: 3516-3524 
    7. Kratz WA, Myers J (1955) Nutrition and growth of several blue-green algae. Am J Bot 42: 282-287 
    8. Lagarde D, Beuf L, Vermaas W (2000) Increased production of zeaxanthin and other pigments by application of genetic engineering techniques to Synechocystis sp. Starin PCC 6803. Appl Environ Microb 66: 64-72 
    9. Murakami A (1997) Quantitative analysis of 77 K fluores-cence emission spectra in Synechocystis sp. PCC 6714 and Chlamydomonas reinhardtii with variable PS I/PS II stoichiometries. Photosynth Res 53: 141-148 
    10. Porter R (1988) DNA transformation. Methods Enzymol 167: 705-706 
    11. Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Muraki A, Nakazaki N, Naruo K, Okumura S, Shimpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803, II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res 3: 109-136 
    12. Anderson JM, Osmond CB (1987) Shade-sun responses: Compromises between acclimation and photoinhibition. In DJ Kyle, CB Osmond, CJ Arntzen, eds, Photoin-hibition. Elsevier, Amsterdam, pp 1-38 
    13. Bennett J (1991) Protein phosphorylation in green plant chloroplasts. Annu Rev Plant Physiol Plant Mol Biol 42: 281-311 
    14. Campbell D, $\"O$quist G (1996) Predicting light acclimation in cyanobacteria from nonphotochemical quenching of photosystem II fluorescence, which reflects state transi-tions in these organisms. Plant Physiol 111: 1293-1298 
    15. Steghens J-P, van Kappel AL, Riboli E, Collombel C (1997) Simultaneous measurement of seven carotenoids, ret-inol and $\alpha$-tocopherol in serum by high-performance liquid chromatography. J Chromatogr B 694: 71-81 
    16. Ke B (2001) The primary electron donor of photosystem II, P680, and photoinhibition. In B Ke, ed, Photosynthesis: Photobiochemistry and Photobiophysics. Kluwer Academic Publishers, Dordrecht, pp 271-288 
    17. Nomsawai P, Tandeu de Marshac N, Thomas JC, Tanticharoen M, Cheevadhanarak S (1999) Light regulation of phycobilisome structure and gene expression in Spir-ulina platensis C1 (Arthrospira sp. PCC 9438). Plant Cell Physiol 40: 1194-1202 
    18. Miskiewicz E, Ivanov AG, Huner NPA (2002) Stoichiometry of the photosynthetic apparatus and phycobilisome structure of the cyanobacterium Plectonema boryanum UTEX 485 are regulated by both light and temperature. Plant Physiol 130: 1414-1425 
    19. $\"O$quist G, Chow WS, Anderson JM (1992) Photoinhibition of photosynthesis represents a mechanism for the long-term regulation of photosystem II. Planta 187: 450-460 
    20. Powles SB (1984) Photoinhibition of photosynthesis induced by visible light. Ann Rev Plant Physiol 35: 15-14 
    21. Samuilov VD, Bezryadnov DB, Gusev MV, Kitashov AV, Fedorenko TA (2001) Hydrogen peroxide inhibits pho-tosynthetic electron transport in cells of cyanobacteria. Biochemistry (Moscow) 66: 640-645 
    22. Vothknecht UC, Soll J (2002) Chloroplast quest: A journey from the cytosol into the chloroplast and beyond. Rev Physiol Biochem Pharmacol 145: 181-222 
    23. Mullineaux CW (1994) Excitation energy transfer from phy-cobilisomes to photosystem I in a cyanobacterial mutant lacking photosystem II. Biochim Biophys Acta 1184 229-242 
    24. Wilde A, Hartel H, Hubschmann T, Hoffmann P, Shesta-kov SV, Borner T (1995) Inactivation of a Synechocystis sp. strain PCC 6803 gene with homology to conserved chloroplast open reading frame 184 increases the photosystem II-to-photosystem I ratio. Plant Cell 7: 649-658 
    25. Bissati KE, Delphin E, Murata N, Etienne A-L, Kirilovsky D (2000) Photosystem II fluorescence quenching in the cyanobacterium Synechocystis PCC 6803: Involvement of two different mechanisms. Biochim Biophys Acta 1457: 229-242 
    26. Chow WS, Osmond CB, Huang L-K (1989) Photosystem II function and herbicide binding sites during photoinhibition of spinach chloroplasts in vivo and in vitro. Photosynth Res 21: 17-26 
    27. Simpson CL, Stern DB (2002) The treasure trove of algal chloroplast genomes, surprises in architecture and gene content, and their functional implications. Plant Physiol 129: 957-966 
    28. Williams JGK (1988) Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803. Method Enzvmol 167: 766-778 
    29. Kim J-H, Lee C-H (2003) Mechanism for photoinactivation of PSII by methyl viologen at two temperatures in the leaves of rice (Oryza sativa L). J Plant Biol 46: 10-16     
    30. Aro E-M, Virgin I, Andersson B (1993) Photoinhibition of photosystem II. Inactivation, protein damage and turn-over. Biochim Biophys Acta 1143: 113-134 
    31. Fujita Y, Murakami A (1987) Regulation of electron transport composition in cyanobacterial photosynthetic system: Stoichiometry among photosystem I and II complexes and their light harvesting antennae and cytochrome b6/f complex. Plant Cell Physiol 28: 1547-1553 
    32. Ochman H, Gerber AS, Hartl DL (1988) Genetic application of an inverse polymerase chain reaction. Genetics 120: 621-623 
    33. Kim J-H, Glick RD, Melis A (1993) Dynamics of photosystem stoichiometry adjustment by light quality in chloro-plasts. Plant Physiol 102: 181-189 
    34. Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In CH Foyer, PM Mul-lineaux, eds, Causes of Photooxidative Stress and Ame-lioration of Defense Systems in Plants. CRC Press, Boca Raton, pp 77-104 
    35. Chow WS, Melis A, Anderson JM (1990) Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. Proc Natl Acad Sci USA 87: 7502-7506 
    36. Chung Y-H, Cho M-S, Moon Y-J, Choi J-S, Yoo Y-C, Park Y-I, Lee K-M, Kang K-W, Park YM (2001) ctr1, a gene involved in a signal transduction pathway of the gliding motility in the cyanobactehum Synechocystis sp. PCC 6803. FEBS Lett 492: 33-38 
    37. Gal A, Zer H, Ohand I (1997) Redox-controlled thylakoid protein phosphorylation, News and views. Physiol Plant 100: 869-885 
    38. Shen G, Vermaas WFJ (1994) Chlorophyll in a Synechocys-tis sp. PCC 6803 mutant without photosystem I and photosystem II core complexes, Evidence for peripheral antenna chlorophylls in cyanobacteria. J Biol Chem 269: 13904-13910 
    39. Nikitishena OV, Smolova TN, Khatypov RA, Shkuropatov AJ, Klimov VV (2002) A new pathway of photoinactiva-tlon of PSll, irreversible photoreduction of pheophytin causes loss of photochemical activity of isolated D1-D2-cytochrome b559 complex. Biochem (Moscow) 67: 364-371 
    40. Demmig-Adams B, Adams WW III (1996) The role of the xanthophyll cycle carotenoids in the protection of photosynthesis. Trends Plant Sci 1: 21-26 
    41. Gantt E (1994) Supramolecular membrane organization, In DA Bryant, ed, The Molecular Biology of Cyanobacteria. Kluwer Academic Publishers, Dordrecht, pp 120-138 
  • 이 논문을 인용한 문헌 (1)

    1. Son, Moon-Ho ; Zulfugarov, Ismayil S. ; Kwon, O-Seob ; Moon, Byoung-Young ; Chung, Ik-Kyo ; Lee, Choon-Hwan ; Lee, Jin-Ae 2005. "The study on the Fluorescence Characteristics of Several Freshwater Bloom Forming Algal Species and Its Application" Algae, 20(2): 113~120     

 저자의 다른 논문

  • Lee, Woo-Sung (11)

    1. 1998 "Decreased Membrane Integrity in Castor Bean Mitochondria by Hydrogen Peroxide. Evidence for the Involvement of Phospholipase D." Journal of plant biology = 식물학회지 41 (3): 178~186    
    2. 1998 "Cloning and DNA Sequencing of Cytosolic Cu/Zn Superoxide Dismutase Gene from Chinese Cabbage" Journal of plant biology = 식물학회지 41 (1): 68~71    
    3. 1999 "Studies on Genes and Biochemical Events Induced by Carbon-Source Starvation in Plant Cells" Journal of plant biology = 식물학회지 42 (4): 321~324    
    4. 1999 "Preferential Use of Glucose during Diauxic Growth of Carrot Cells Growing on Glucose and Malate" Journal of plant biology = 식물학회지 42 (3): 226~231    
    5. 2000 "DNA Endonuclease- and Active Oxygen-Associated Degradation of Chloroplast DNA in Response to Paraquat-Induced Oxidative Stress" Journal of plant biology = 식물학회지 43 (4): 226~231    
    6. 2000 "Modulation of Interleukin Production in Anthrax Lethal Toxin-treated Macrophages by Melatonin and Dehydroepiandrosterone" Journal of biochemistry and molecular biology = 한국생화학회지 33 (6): 463~468    
    7. 2001 "Cloning and Expression of a cDNA Encoding Aminoalcoholphosphotransferase from Pimpinella brachycarpa" Journal of plant biology = 식물학회지 44 (1): 27~32    
    8. 2001 "Different Susceptibilities to Low Temperature Photoinhibition in the Photosynthetic Apparatus Among three Cultivars of Cucumber (Cucumis sativus L.)" Journal of photoscience : an international journal officail organ of the korean society of photoscience 8 (3): 105~112    
    9. 2004 "Phylogenetic Analysis of Harmful Algal Bloom (HAB)-Causing Dinoflagellates Along the Korean Coasts, Based on SSU rRNA Gene" Journal of microbiology and biotechnology 14 (5): 959~966    
    10. 2010 "Auxin and Abscisic Acid Responses of Auxin Response Factor 3 in Arabidopsis Lateral Root Development" Journal of plant biology = 식물학회지 53 (2): 150~154    
  • Lee, Choon-Hwan (39)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • NDSL :

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기