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Journal of plant biology = 식물학회지 v.47 no.4, 2004년, pp.289 - 299   피인용횟수: 1
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Selection and Characterization of Transposon Tagging Mutants of Synechocystis sp. PCC 6803 Sensitive to High-Light and Oxidative Stresses

Song, Eun-Kyeong    (Department of Molecular Biology, Pusan National University   ); Zulfugarov, Ismayil S.    (Department of Molecular Biology, Pusan National University   ); Kim, Jin-Hong    (Department of Molecular Biology, Pusan National University   ); Kim, Eun-Ha    (Department of Molecular Biology, Pusan National University   ); Lee, Woo-Sung    (Department of Biological Science, Sungkyunkwan University   ); Lee, Choon-Hwan    (Department of Molecular Biology, Pusan National University  );
  • 초록

    We compared several analytical tools to identify which were most applicable for the selection and characterization of specific transposon-tagged mutant strains of Synechocystis sp. PCC 6803 that are sensitive to high light and oxidative stresses. Our primary parameter was the maximum photochemical efficiency of dark-adapted cells, a very sensitive factor that can be determined in a non-destructive manner. Using this as a tool for primary selection, we identified five mutant strains with different sensitivities to photoinhibition and photooxidation. For further characterization, we obtained data describing the absorption spectra for pigment contents, the 77 K fluorescence spectra, non-photochemical quenching (as a down-regulation process), and the photosynthetic electron transfer rate. Based on these results, we were able to design a strategy for selecting mutants with specific phenotypes. Here, we also discuss the strengths and weaknesses of each selection and characterization tool.


  • 주제어

    chlorophyll fluorescence .   cyanobacterium .   high light .   oxidative stree .   photoinhibition.  

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    1. Son, Moon-Ho ; Zulfugarov, Ismayil S. ; Kwon, O-Seob ; Moon, Byoung-Young ; Chung, Ik-Kyo ; Lee, Choon-Hwan ; Lee, Jin-Ae 2005. "The study on the Fluorescence Characteristics of Several Freshwater Bloom Forming Algal Species and Its Application" Algae, 20(2): 113~120     

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