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Journal of plant biology = 식물학회지 v.47 no.4, 2004년, pp.314 - 321   피인용횟수: 5
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Alterations in the Photosynthetic Pigments and Antioxidant Machineries of Red Pepper (Capsicum annuum L.) Seedlings from Gamma-Irradiated Seeds

Kim, Jin-Hong    (Division of Radiation Application Research, Korea Atomic Energy Research Institute   ); Baek, Myung-Hwa    (Division of Radiation Application Research, Korea Atomic Energy Research Institute   ); Chung, Byung-Yeoup    (Division of Radiation Application Research, Korea Atomic Energy Research Institute   ); Wi, Seung-Gon    (Division of Radiation Application Research, Korea Atomic Energy Research Institute   ); Kim, Jae-Sung    (Division of Radiation Application Research, Korea Atomic Energy Research Institute  );
  • 초록

    To characterize the stimulatory effects of low-dose gamma radiation on early plant growth, we investigated alterations in the photosynthesis and antioxidant capacity of red pepper (Capsicum annuum L.) seedlings produced from gamma-irradiated seeds. For two cultivars (Yeomyung and Joheung), three irradiation groups (2, 4, and 8 Gy, but not 16 Gy) showed enhanced development, although Fv/Fm, the maximum photochemical efficiency of Photosystem II (PSII), did not differ significantly among any of the four groups. In contrast, values for 1/Fo - 1/Fm, i.e., a measure of functional PSII content, decreased in the irradiated groups of `Yeomyung' but increased in those of `Joheung'. Pigment analyses and enzyme activity assays revealed that irradiation altered the compositions of photosynthetic pigments (chlorophylls and carotenoids) as well as the activities of antioxidant enzymes (superoxide dismutase and glutathione reductase). However, these shifts were not directly related to the increase in early growth, although they were cultivar- and developmental stage-dependent. In addition, the effects of irradiation on the enzymatic activities measured here were at opposition between the two cultivars.


  • 주제어

    antioxidant enzyme .   carotenoid .   chlorphyll a fluorescence .   low-dose radiation .   photosynthesis.  

  • 참고문헌 (53)

    1. AI-Safadi B, Simon PW (1996) Gamma irradiation-induced variation in carrots (Daucus carota L.). J Amer Soc Hort Sci 121: 599-603 
    2. Beyer WF, Fridovich Y (1987) Assaying for superoxide dis-mutase activity: Some large consequences of minor changes in conditions. Anal Biochem 161: 559-566 
    3. Bishop NI (1996) The $\beta,\varepsilon$ -carotenoid, lutein, is specifically required for the formation of the oligomeric forms of the light harvesting complex in the green alga, Scene-desmus obliquus. J Photochem Photobiol B: Biol 36: 279-283 
    4. Charbaji T, Nabulsi I (1999) Effect of low doses of irradiation on in vitro growth of grapevine. Plant Cell Tiss Org Cult 57: 129-132 
    5. Demmig-Adams B, Gilmore AM, Adams III WW (1996) In vivo functions of carotenoids in higher plants. FASEB J 10:403-412 
    6. Edge R, McCarvey DJ, Truscott TG (1997) The carotenoids as anti-oxidants: A review. J Photochem Photobiol B: Biol 41: 189-200 
    7. Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 90: 87-92 
    8. Kim J-S, Lee H-Y, Baek M-H, Kim J-H, Kim SY (2002b) Effects of low dose $\gamma$ radiation on the dormancy, growth and physiological activity of seed potato (Solanum tuberosum L). J Kor Soc Hort Sci 43: 596-602 
    9. Kim S-J, Lee C-H, Hope AB, Chow WS (2001 b) Inhibition of photosystem I and II and enhanced back flow of photosystem I electrons in cucumber leaf discs chilled in the light. Plant Cell Physiol 42: 842-848 
    10. Lee H-Y, Kim J-S, Baek M-H, Park S-C, Park Y-I (2002a) Effects of low dose $\gamma$ -radiation on photosynthesis of red pepper (Capsicum annuum L.) and the reduction of photoinhibition. Kor J Environ Agr 21: 83-89     
    11. Miller MW (1987) Radiation hormesis in plants. Health Physiol 52: 607-616 
    12. Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59: 1011-1012 
    13. Taguchi Y, Tsutsumi N,.Tatara A, Eguchi H, Tano S (1994) Effects of low-dose $\gamma$ -irradiation on the root apical mer-istem of barley. Environ Mut Res Commun 16: 205-209 
    14. Wada H, Koshiba T, Matsui T, Sato M (1998) Involvement of peroxidase in differential sensitivity to $\gamma$-radiation in seedlings of two Nicotiana species. Plant Sci 132: 109-119 
    15. Larson RA (1988) The antioxidants of higher plants. Phy-tochem 27: 969-978 
    16. Mullineaux PM, Creissen GP (1997) Glutathione reduc-tase: Regulation and role in oxidative stress, In JG Scan-dalios, ed, Oxidative Stress and the Molecular Biology of Antioxidaht Defenses. Cold Spring Harbor Labora-tory Press, New York, pp 667-713 
    17. Rivas ADL, Telfer A, Barber J (1993) Two coupled $\beta$ -caro-tene molecules protect P680 from photodamage in iso-lated photosystem II reaction centres. Biochim Biophys Acta 1142: 155-164 
    18. McKersie BD, Chen Y, Beus MD, Bowley SR, Bowler C, Inze D, D' Halluin K, Botterman J (1993) Superoxide dismutase enhances tolerance of freezing stress in trans-genie alfalfa (Medicago sativa L.). Plant Physiol 103: 1155-1163 
    19. Thiede ME, Link SO, FeIlows RJ, Beedlow PA (1995) Effects of gamma radiation on stem diameter growth, carbon gain and biomass partitioning in Helianthus annuus. Environ Exp Bot 35: 33-41 
    20. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72: 248-254 
    21. Thiele A, Krause GH (1994) Xanthophyll cycle and ther-mal energy dissipation in photosystem II: Relationship between zeaxanthin formation, energy-dependent fluo-rescence quenching and photoinhibition. J Plant Physiol 144: 324-332 
    22. Lee H-Y, Kim J-S, Baek M-H, Lee Y-K, Im D-S (2002b) Effects of low dose $\gamma$ -radiation on the growth, activities of enzymes and photosynthetic activities of gourd (Lagenaria siceraria). Kor J Environ Biol 20:197-204 
    23. Slooten L, Capiau K, Camp WV, van Montagu M, Sybesma C, Inze D (1995) Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overex-pressing manganese superoxide dismutase in the chlo-roplasts. Plant Physiol 107: 737-750 
    24. Frank HA, Cogdell RJ (1993) The photochemistry and func-tion of carotenoids in photosynthesis, In A Young, G Britton, eds, Carotenoids in Photosynthesis. Chapman & Hall, London, pp 252-326 
    25. Gupta AS, Heinen JL, Holaday AS, Burke JJ, Allen RD (1993) Increased resistance to oxidative stress in trans-genie plants that overexpress chloroplastic Cu/Zn super-oxide dismutase. Proc Natl Acad Sci USA 90: 1629-1633 
    26. Kim J-S, Lee E-K, Baek M-H, Kim D-H, Lee YB (2000) Influence of low dose $\gamma$ radiation on the physiology of germinative seed of vegetable crops. Kor J Environ Agr 19:58-61 
    27. Niyogi KK (1997) The roles of specific xanthophylls in photoprotection. Proc Natl Acad Sci USA 94: 14162-14167 
    28. Fryer MJ, Andrews JR, Oxborough K, Blowers DA, Baker NR (1998) Relationship between $CO_2$ assimilation, photosynthetic electron transport, and active $O_2$ metabolism in leaves of maize in the field during peri-ods of low temperature. Plant Physiol 116: 571-580 
    29. Conter A, Dupouy D, Delteil C, Planel H (1986) Influence of very low doses of ionizing radiation on Synechococ-cus lividus metabolism during the initial growth phase. Arch Microbiol 144: 286-290 
    30. Lee EK, Kim J-S, Lee Y-K, Lee YB (1998) Effect of low dose $\gamma$ -ray irradiation on the germination and growth in red pepper (Capsicum annuum L.). J Kor Soc Hort Sci 39: 670-675 
    31. Asada K (1999) The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50: 601-639 
    32. Calabrese EJ (2002) Hormesis: Changing view of the dose-response, a personal account of the history and current status. Mutat Res 511:181-189 
    33. Chakravarty B, Sen S (2001) Enhancement of regeneration potential and variability by $\gamma$ -irradiation in cultured cells of Sclla indica. Biol P1ant 44: 189-193 
    34. Kim J-S, Kim J-K, Lee Y-K, Baek M-W, Kim J-G (1998) Effects of low dose gamma radiation on the germination and yield components of Chinese cabbage. Kor J Envi-ron Agr 17: 274-278 
    35. Lee H-Y, Chow WS, Hong YN (1999) Photoinactivation of photosystem II in leaves of Capsicum annuum. Physiol Plant 105: 377-384 
    36. Okamoto H, Tatara A (1995) Effects of low-dose $\gamma$ -irradia-tion on the cell cycle duration of barley roots. Environ Exp Bot 35: 379-388 
    37. Luckey TD (1980) Hormesis with Ionizing Radiation. CRC Press, Boca Raton, pp 32-38 
    38. Koepp R, Kramer M (1981) Photosynthetic activity and dis-tribution of photoassimilated $^{14}C$ in seedlings of Zea mays grown from gamma-irradiated seeds. Photosyn-thetica 15: 484-489 
    39. Lee H-Y, Kim J-S, Baek M-H, Yoo J-C, Kwon S-T (2003) Effects of low dose $\gamma$ irradiation on physiological activi-ties of radish (Raphanus sativus) during early growth and reduction of ultravioIet-B stress. J Kor Soc Hort Sci 44: 314-320 
    40. Zaka R, Vandecasteele CM, Misset MT (2002) Effects of low chronic doses of ionizing radiation on antioxidant enzymes and $G_6PDH$ activities in Stipa capillata (Poaceae). J Exp Bot 53: 1979-1987 
    41. Kim J-H, Lee C-H (2003) Mechanism for photoinactivation of Psll by methyl viologen at two temperatures in the leaves of rice (Oryza sativa L). J Plant Biol 46: 10-16     
    42. Kim J-S, Baek M-H, Lee H-Y, Lee Y-K (2001 a) Effects of low dose gamma irradiation on the germination and physio-logical activity of red red pepper (Capsicum annuum L.) seed. J Kor Assoc Rad Prot 26: 409-415 
    43. Wiendl FM, Wiendl FW, Wiendl JA, Vedovatto A, Arthur V (1995) Increase of onion yield through low dose of gamma irradiation of its seeds. Rad Phys Chem 46: 793-795 
    44. Demmig-Adams B, Adams III WW (1996) The role of xan-thophyll cycle carotenoids in the protection of photo-synthesis. Trends Plant Sci 1: 21-26 
    45. Gilmore AM (1997) Mechanistic aspects of xanthophyl cycle-dependent photoprotection in higher plant chlo-roplasts and leaves. Physiol Plant 99: 197-209 
    46. Gilmore AM, Yamamoto HY (1991) Resolution of lutein and zeaxanthin using a nonencapped, lightly carbon-loaded C-18 high-performance liquid chromatographic column. J Chromatogr 543: 137-145 
    47. Kim J-S, Baek M-H, Lee Y-K, Lee H-Y, Yoo J-C (2002a) Effect of low-dose gamma radiation to enhance germi-nation rate in bottle gourd and pumpkin seeds. Kor J Environ Agr 21: 202-207     
    48. AI-Safadi B, Ayyoubi Z, Jawdat D (2000) The effect of gamma irradiation on potato microtuber production in vitro. Plant Cell Tiss Org Cult 61: 183-187 
    49. Stan S, Croitoru A (1970) Effect of low, moderate and high levels of gamma radiations $(^{60}Co)$ on soybean plants. I. Analysis of growth and yield. Stim Newsl 1: 23-25 
    50. Telfer A, Dhami S, Bishop SM, Phillips D, Barber J (1994) $\beta$ -carotene quenches singlet oxygen formed by iso-lated photosystem II reaction centers. Biochem 33: 14469-14474 
    51. Eidus LK (2000) Hypothesis regarding a membrane-associ-ated mechanism of biological action due to low-dose ionizing radiation. Rad Environ Biophys 39: 189-195 
    52. Kim J-H, Lee C-H (2002) Decrease of photochemical effi-ciency induced by methyl viologen in rice (Oryza sativa L.) leaves is partly due to the down-regulation of PSII. J Photosci 9: 65-70 
    53. Luckey TD (1991) Radiation Hormesis. CRC Press, Boca Raton, pp 9 
  • 이 논문을 인용한 문헌 (5)

    1. 2004. "" 환경생물 = Korean journal of environmental biology, 22(4): 537~542     
    2. 2005. "" Journal of plant biology = 식물학회지, 48(3): 276~283     
    3. 2005. "" Journal of plant biology = 식물학회지, 48(1): 47~56     
    4. 2008. "" Journal of plant biology = 식물학회지, 51(1): 52~57     
    5. 2008. "" 환경생물 = Korean journal of environmental biology, 26(1): 15~21     

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