본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biology = 식물학회지 v.47 no.4, 2004년, pp.356 - 360   피인용횟수: 2
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Effect of Polyploidy Induction on Biomass and Ginsenoside Accumulations in Adventitious Roots of Ginseng

Kim, Yun-Soo    (Korea Ginseng Institute, Chung-Ang University   ); Hahn, Eun-Joo    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University   ); Murthy, Hosakatte Niranjana    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Department of Botany, Karnatak University   ); Paek, Kee-Yoeup    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University  );
  • 초록

    Adventitious roots of Panax ginseng C.A. Meyer (a natural tetraploid) were treated with 50 or 100 mg L-1 colchicine for 12, 24, 36, 48, or 60 h to induce polyploid (octoploid) roots. The largest number of octoploid roots was obtained with a 100 mg L-1 colchicine treatment over 60 h. To verify that ginsenoside was being accumulated in the developing tissues, the tetraploid (control) and octoploid roots were cultured for 40 d in Murashige and Skoog media that lacked NH4NO3 but was supplemented with 2 mg L-1 naphthaleneacetic acid and 50 g L-1 sucrose. Levels of fresh and dry biomass were greater in the octoploid roots. Although total ginsenoside and Rb-group ginsenoside contents were less in the octoploid roots than in the tetraploids, the former had a higher amount of Rg-group ginsenosides (especially Rg1). These results demonstrate the benefit that polyploid adventitious roots provide in enhancing the production of secondary metabolites in ginseng.


  • 주제어

    colchicine .   octoploid .   Panax ginseng C.   A.   Meyer .   tetraploid.  

  • 참고문헌 (14)

    1. Chen XC, Zhu YG, Zhu LA, Huang C, Chen Y, Chen LM, Fang F, Zhou YC, Zhao CH (2003) Ginsenoside Rg1 attenuates dopamine-induced apoptosis in PC12 cells by suppressing oxidative stress. Eur J Pharmacol 473: 1-7 
    2. Svehlikova V, Repcak M (2000) Variation of apigenin quantity in diploid and tetraploid Cbamomtlla rectita (L.) Rauschert. Plant Biol 2: 403-407 
    3. Shin YH, Kim SC, Han JW, Oh JY, Jeong OM, Shin DH, Nah SY (1997) Cinseng protopanaxatriol saponins but not protopanaxadiol saponins inhibit spontaneous motility of intestine. Koryo Insam Hakheochi 21: 35-38 
    4. Bulgakov VP, Lauve LS, Tchernoded GK, Khodakovskaya MV, Zhuravlev YN (2000) Chromosome variation in ginseng cells transformed with the rolC plant oncogene. Russ J Genet 36: 150-156 
    5. Griesbach RJ, Kamo KK (1996) The effect of induced polyploidy on the flavonoids of Petunia Mitchell. Phytochem42: 361-363 
    6. Grushvitskii IV (1961) Zhenshen: Voprosy Biologii (Gin-seng: Problems in Biology), Leningrad 
    7. Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax gin-seng C. A. Meyer. In Vitro Cell Dev Biol Plant 39: 245-249 
    8. De Jesus-Gonzalez L, Weathers PJ (2003) Tetraploid Artemisia annua hairy roots produce more artemisinin than diploids, Plant Cell Rep 21: 809-813 
    9. Palazon J, Cusido RM, Bonfill M, Mallol A, Moyano E, Morales C, Pinol MT (2003) Elicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production. Plant Physiol Biochem 41: 1019-1025 
    10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473-497 
    11. Gao SL, Zhu DN, Cai ZH, Xu DR (1996) Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell Tiss Org Cult 47: 73-77 
    12. Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11: 211-215 
    13. Zheng GZ, Yang CR (1994) Sanchi (Panax notoginseng) Biology and Application. Science Press, Beijing 
    14. Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: A review. Euphytica 87: 81-89 
  • 이 논문을 인용한 문헌 (2)

    1. 2005. "" Journal of plant biology = 식물학회지, 48(2): 258~262     
    2. 2009. "" Journal of ginseng research = 高麗人參學會誌, 33(1): 65~71     

 저자의 다른 논문

  • 김윤수 (7)

    1. 2004 "다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향" 식물생명공학회지 = Korean journal of plant biotechnology 31 (3): 249~253    
    2. 2004 "구기자나무 (Lycium chinense)의 모상근 유도 및 생물반응기 배양" 식물생명공학회지 = Korean journal of plant biotechnology 31 (4): 295~300    
    3. 2005 "생물반응기내의 공기주입량 및 Sparger 형태가 인삼 (Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 함량에 미치는 영향" 식물생명공학회지 = Korean journal of plant biotechnology 32 (2): 111~116    
    4. 2005 "홍경천 (Rhodiola sachalinensis A. Bor) 잎절편으로부터 식물체 재분화에 미치는 Cytokinin과 Putresine의 영향" 식물생명공학회지 = Korean journal of plant biotechnology 32 (3): 195~199    
    5. 2005 "Methyl jasmonate 처리에 의한 인삼 (Panax ginseng C.A. Meyer) 부정근의 이차대사산물 및 항산화활성 증가" 식물생명공학회지 = Korean journal of plant biotechnology 32 (3): 225~231    
  • 한은주 (24)

  • Paek, Kee-Yoeup (94)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기